The Effect of Prunus persica Batch var. davidiana Max. Hot-Water Extract on the Lipid Peroxide and Creatine Phosphokinase Activity in Streptozotocin-Induced Diabetic Rats

당뇨성 흰쥐의 과산화지질 및 Creatine Phosphokinase 활성에 돌복숭아(Prunus persica Batsch var. davidiana Max ) 열수 추출액이 미치는 영향

  • Kim Han-Soo (Department of Biotechnology, Miryang National University)
  • 김한수 (밀양대학교 생명공학과)
  • Published : 2005.09.01

Abstract

The purpose of this study was designed to observe the effects of the Prunus persica Batsch var. davidiana Max. hot-water extract on the improvement of the glucide and lipid metabolism in the serum of streptozotocin (STZ, 55, mg/kg B.W., I.P. injection)-induced diabetic rats(S.D. strain, male) fed the experimental diets for 5 weeks. Electrolyte(Na, K, Cl) concentration in serum were fairly reduced in the group BSP(basal diet+STZ+Prunus persica $5.0g\%$ extract) than in the STZ(I.P.)-induced diabetic rats group(group BSW, basal diet+STZ(I.P.)+water). Although there was no significant difference among the groups. Concentrations of free fatty acid and lipid peroxide in serum were significantly higher in the STZ-induced diabetic group(group BSW) and STZ+Prunus persica $5.0;g\%$ extract group(group BSP) than those in the control group(group BW, basal diet+water). However, the concentrations of free fatty acid and lipid peroxide in serum were remarkably reduced in the group BSP than those in the group BSW, The activity of creatine phophokinase In serum was significantly lower in the group BSP than in the group BSW However, the activity of LCAT in serum was increased in the group BSP(Prunus persica $5.0\;g\%$ hot-water extract administration group) than in the STZ-induced diabetic group(group BSW). The above results shows that Prunus persica Batsch var. davidiana Max. were effective on the improvement of the glucide and lipid metabolism in serum of streptozotocin-induced diabetic rats.

돌복숭아(Prunus persica Batsch var. davidiana Max.)의 생리활성 추출물질($5.0g\%$ extract)이 streptozotocin (STZ, 55mg/kg B.W., I.P. injection)으로 유발된 당뇨성 Sprague Dawley계 수컷 흰쥐의 지질 및 당질대사 이상에 의해 야기되는 각종 질환의 예방과 개선 효과 등에 많은 생리적 효능이 있을 것으로 판단되어 본 실험을 수행하였다. 기본 식이에 물 급여군인 대조군(BW군)을 비롯한 STZ 당뇨 유발 흰쥐에 물 급여군(BSW군)과 당뇨 유발군에 돌복숭아 추출액을 급여한 군(BSP군)을5주간실험 사육하였다. 혈청 중의 전해질 농도(Na, K, Cl)는 당뇨 유발군(BSW군)에 돌복숭아 추출액을 급여(BSP군)하므로서 농도가 다소 나마 감소되는 것으로 나타났지만 유의성 있는 농도의 차이는 없었다. 한편 혈청 유리지방산 및 과산화지질 농도, creatine phosphokinase활성 등에서 돌복숭아추출액을 급여하므로서 유의적인 농도와 활성 감소를 나타내었고, 혈청 lecithin cholesterol acyltransferase(LCAT) 활성은 당뇨 유발군에 돌복숭아 추출액 급여에 의하여 상승되는 것으로 나타났다. 따라서 돌복숭아 중의 생리활성물질이 STZ으로 유발된 당뇨성 흰쥐의 당질 및 지질대사 이상 등에서 오는 각종 질환의 예방 및 치료에 개선 효과가 있을 것으로 생각된다.

Keywords

References

  1. Laybutt, DR, Kaneto, H, Hasenkamp, W, Grey, S, Jonas, JC, Sgroi, DC, Groff, A, Ferran, C, Bonner, WS, Shama, A and Weir, GC. Increased expression of antioxidant and antiapoptotic genes in islets that may contribute to ${\beta}$-cell survival during chronic hyperglycemia. Diabetes 51:413-423. 2002 https://doi.org/10.2337/diabetes.51.2.413
  2. Wolff, SP. Diabetes mellitus and free radicals, transition metals and oxidative stress in the aetiology of diabetes mellitus and complications. Br. Med. Bull. 49:642-652. 1993 https://doi.org/10.1093/oxfordjournals.bmb.a072637
  3. Macrae, R, Robinson, RK and Sadler, MJ. Encyclopedia of food science, food technology and nutrition, Vol. II pp.1329. Academic Press, New York. 1993
  4. Yoon, JW, Kim, CJ, Park, CY and McArthur, RG. Effect of environmental factors on development of insulin - dependent diabetes mellitus. Clin. Invest. Med. 10:459-466. 1987
  5. Zachania, M. Effect of brown rice and soybean dietary fiber on the control of glucose and lipid metabolism in diabetic rats. Am. J. Clin. Nutr. 38:388-393. 1983
  6. Cho, YJ and Bang, MA. Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Kor. J. Nutr. 37:5-14. 2004
  7. Kim, HS and Chung, SY. Effects of feeding mixed oils of butter, sardine and safflower on the lipid components in serum and activities of hepatic functional enzyme in rats. J. Kor. Soc. Food Nutr. 21: 608-616. 1992
  8. Kim, HS, Kim, SH, Kim, GJ, Choi, WJ and Chung, SY. Effects of the feeding mixed oils with various level of n-3 and n-6 polyunsaturated fatty acid on the lipid components of liver, brain, testis and kidney in dietary hyperlipidemic rats. J. Kor. Soc. Food Nutr. 22:685-691. 1993
  9. Lee, TH. Diagnosis and classification of diabetes mellitus. Food Indus. and Nutr. 4:61-65. 1999
  10. The expert committee on the diagnosis and classification of diabetes mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 20(suppl. 7):1183-1197. 1997 https://doi.org/10.2337/diacare.20.7.1183
  11. Son, HS. Exercise physiology and diabetes : The riskbenefit profile. Kor. J. Med. 68:603-610. 2005
  12. Harold, J and Holler, RD. Diabetes medical nutrition therapy, A professional guide to management and nutrition education resources. J Am. Diet. Assoc. 97:99113. 1997
  13. American Diabetes Association. Nutrition recommendations and principles for people with diabetes mellitus (position statement). Diabetes Care 21 (suppl.1): s32- s35. 1998 https://doi.org/10.2337/diacare.21.1.32
  14. 신길구. 신씨본초학, pp.562-564. 수문사. 1973
  15. 안경환. 도해고금한방총람, pp.205-206. 서원당. 1980
  16. 한국생양약학교수협의회 편저. 본초학, pp.526-528. 대한약사회. 1994
  17. Kim, HS. Effects of the Prunus persica Batsch var. davidiana Max. extract on the blood glucose and serum lipid components in streptozotocin induced diabetic rats. Kor. J. Food & Nutr. 17:337-345. 2004
  18. Youk, KC and Youn, SH. Effect of rapit weight reduction on physical fitness and electrolyte. Kor. J. Physi. Edu. 42:719-727. 2003
  19. Hene, RJ. Effect of high-dose aldosterone infusion on renal electrolyte excretion in patients with renal insufficiency. Am. J. Neph. 7:33-38. 1987 https://doi.org/10.1159/000167426
  20. Kim, KH. A translation: The clinical application of the results of the test, pp.178-183. Ko Moon Sa, Seoul, Korea. 1980
  21. Yi, KN and Rhee, CS. Clinical pathology file, pp. 128-131. Euihak Munwhasa Co, Seoul, Korea. 1996
  22. Prakash, R, Parmley, WW and Horvat, M. Serum cortisol, plasma free fatty acids and urinary catecholamines as indicators of complications in acute myocardial infarction. Circulation 45:736-746. 1972 https://doi.org/10.1161/01.CIR.45.4.736
  23. Lim, SJ and Kim, YR. Effects of Benincasa hispida seeds intake on blood glucose and lipid levels in streptozotocin induced diabetic rats. Kor. J. Nutr. 37: 259-265. 2004
  24. Choi, JW, Sohn, KH and Kim, SH. Effects of nicotinamide on the serum lipid composition in streptozotocin induced diabetic rats. J. Kor. Soc. Food Nutr. 20:306-311. 1991
  25. Reiter, RJ. The role of the neurohormone melatonin as a buffer against macromolecular oxidative damage. Neurochem. International. 27:453-460. 1995
  26. Kelly, FJ. Use of antioxidants in the prevention and treatment of disease. J. Int. Fed. Cin. Chem. 10:21-23. 1998
  27. Seo, JS, Lee, KS and Jang, JH. Effect of dietary supplementation of ${\beta}$-carotene on lipid peroxide level and antioxidative vitamins of diabetic rats. J. Kor. Soc. Food Sci. Nutr. 33:72-77. 2004 https://doi.org/10.3746/jkfn.2004.33.1.072
  28. Lee, JW, Kim, JE, Park, IW, Lim, SG, Song, KE, Cho, HK, Shin, GT, Kim, HS and Kim, KM. A study on the appropriate normal range of serum creatinine level for Koreans. Kor. J. Nephrol. 23:721-728. 2004
  29. Kim, JI, Cho, SJ, Lee, YI, Bae, DS, Lee, SJ and Kim, JS. The serum NPN, BUN and creatinine values in the patient with congestive heart failure. Kor. J. Inter. Med. 27:145-149. 1984
  30. Whanger, PD, Weswig, PH, Schmitz, JA and Oldfield, JE. Effects of selenium and vitamin E deficiencies on reproduction, growth, blood components and tissue lesions in sheep fed purified diets. J. Nutr. 107:12881297. 1977
  31. Yi, KN and Rhee, CS. Clinical pathology file, pp. 245-250. Euihak Munwhasa Co, Seoul, Korea. 1996
  32. Do, SH, Kwack, KJ, KO, H and Han, BM. A study on the peri operative changes of creatine kinase and lactic dehydrogenase. J. Kor. Soc. Anesth. 24:56-59. 1991 https://doi.org/10.4097/kjae.1991.24.1.56
  33. Lee, JJ, Han, IK, Choi, YJ, Kang, JS and Chang, YS. Effects of dietary lipid sources and levels on lecithin cholesterol acyltransferase activity and cholesterol metabolism in rats. Kor. J. Nutr. 26:131-144. 1993
  34. Glomset, JA. Physiological role of lecithin cholesterol acyltransferase. Am. J. Clin. Nutr. 23:1129-1134. 1970