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The Pharmaceutical Society of Korea (대한약학회)
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Mutant Presenilin 2 Increases Acetylcholinesterase Activity in Neuronal Cells

  • Nguyen Hong Nga (College of Pharmacy, Chungbuk National University) ;
  • Hwang Dae Youn (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Kim Young Kyu (National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Yoon Do Young (Lab of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Jae Hwa (Lab of Cell Biology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee Moon Soon (National Institute of Environmental Research) ;
  • Lee Myung Koo (College of Pharmacy, Chungbuk National University) ;
  • Yun Yeo Pyo (College of Pharmacy, Chungbuk National University) ;
  • Oh Ki Wan (College of Pharmacy, Chungbuk National University) ;
  • Hong Jin Tae (College of Pharmacy, Chungbuk National University)
  • Published : 2005.09.01
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Abstract

A presenilin 2 mutation is believed to be involved in the development of Alzheimer's disease. In addition, transgenic mice with a presenilin 2 mutation have been reported to have learning and memory impairments. In this study, exposing PC12 cells expressing mutant presenilin 2 to $50{\mu}M\;A{\beta}_{25-35},\;30mM$ L-glutamate and $50{\mu}M\;H_2O_2$ caused a significant increase in acetylcholine esterase activity. An in vivo study revealed high levels of this enzyme activity in the mutant presenilin 2 transgenic brains compared with the wild type presenilin 2 transgenic and non-transgenic samples. These results suggest that a mutant presenilin 2-induced neurodegeneration in Alzheimer's disease might be involved in the increase in acetylcholinesterase activity. These findings might help in the development of an appropriate therapeutic intervention targeting mutant presenilin 2-induced Alzheimer's disease.

Keywords

References

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