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The Pharmaceutical Society of Korea (대한약학회)
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A Potent Anti-Complementary Acylated Sterol Glucoside from Orostachys japonicus

  • Yoon, Na-Young (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Min, Byung-Sun (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hyeong-Kyu (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jong-Cheol (Department of Oriental Medicine Resource, Suncheon National University) ;
  • Choi, Jae-Sue (Faculty of Food Science and Biotechnology, Pukyong National University)
  • Published : 2005.08.01
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Abstract

In order to isolate substances that inhibit the hemolytic activity of human serum against eryth-rocytes, we have evaluated whole plants of the Orostachys japonicus species with regard to its anti-complement activity, and have identified its active principles following activity-guided isolation. A methanol extract of the O. japonicus, as well as its n-hexane soluble fraction, exhibited significant anti-complement activity on the complement system, which was expressed as total hemolytic activity. A bioassay-guided chromatographic separation of the constituents resulted in the isolation of three known compounds 1-3 from the active n-hexane fraction. The structure of these compounds were analyzed, and they were identified as hydroxyhopanone (1), $\beta-sitosteryl-3-O-\beta-D-glucopyranosyl-6'-O-palmitate$ (2), and $\beta-sitosteryl-3-O-\beta-D-glucopyranoside$ (3), respectively. Of these compounds, compound 2 exhibited potent anti-complement activity $(IC_{50}=1.0\pm0.1{\mu}M)$ on the classical pathway of the complement, as compared to tiliroside $(IC_{50}=76.5\pm1.1{\mu}M)$, which was used as a positive control. However, compounds 1 and 3 exhibited no activity in this system.

Keywords

References

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