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Helicobacter pylori에 의한 위세포독성 및 interleukin-8 생성에 미치는 무의 억제효과

Inhibitory Effect of Radish on Gastric Cell Toxicity and Interleukin-8 Production Induced by Helicobacter pylori

  • 손윤희 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 서정일 (동국대학교 의과대학 내과학교실) ;
  • 박인경 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소) ;
  • 황철원 (한동대학교 생명공학부) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 남경수 (동국대학교 의과대학 약리학교실 및 난치병한양방치료연구소)
  • Shon Yun Hee (Department of Pharmacology, College of Medicine and Interactable Disease Research Center Dongguk University) ;
  • Suh Jeong Ill (Department of Internal Medicine, College of Medicine Dongguk University) ;
  • Park In Kyung (Department of Pharmacology, College of Medicine and Interactable Disease Research Center Dongguk University) ;
  • Hwang Cher Won (Department of Enviromental Microbiology, Handong University) ;
  • Kim Cheorl Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine Dongguk University) ;
  • Nam Kyung Soo (Department of Pharmacology, College of Medicine and Interactable Disease Research Center Dongguk University)
  • 발행 : 2005.08.01

초록

무(한국 품종 및 일본 품종)를 사용하여 H. pylori에 의한 위세포독성 및 IL-8생성에 미치는 영향을 살펴보았다. 그 결과 무는 H. pylori에 의한 위세포독성을 농도의존적으로$(2\~10 mg/ml)$ 억제하였으며 또한 위암세포인 KATO III에서 분비되는 IL-8의 생성을 억제시킴$(5\~10mg/ml)$을 알았다. 이러한 사실은 무가 H. pylori감염에 의한 위점막세포의 손상을 방지할 수 있음을 나타낸다.

The efforts of Korean and Japanese radishes on the viability and interleukin (JL)-8 production by Helicobacter pylori were investigated in human gastric epithelial cell. Cell viability was significantly decreased when they were incubated with H. pylori toxin (p <0.05, p<0.01 and p<0.005). Co-incubation with Korean or Japanese radish increased H. pylori toxin-inhibited cell growth in a concentration-dependent manner. The production of IL-8 was greatly increased in H. pylori-infected gastric epithelial cell in concentration- and time-dependent manners. The increased production of IL-8 was significantly inhibited by Korean (p<0.05 and p<0.01) or Japanese (p<0.05) radishes $(5\~10mg/ml)$. These results indicate that Korean and Japanese radishes have protective effects on H. pylori-inhibited cell growth and H. pylori-induced gastric mucosal cell inflammation by suppressing the production of inflammatory cytokine (IL-8) from gastric epithelial cell.

키워드

참고문헌

  1. Baggiolini, M, A. Walz and S. L. Kunkel. 1989. Neutrophilactivating peptide-1/interleukin-8: a novel cytokine that activates neutrophils. J. Clin. Invest. 84, 1045-1049 https://doi.org/10.1172/JCI114265
  2. Blaser, M. J. 1992. Hypothesis on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102, 720-727
  3. Blaser, M. J., G. I. Perez-Perez, H. Kleanthous, T. L. Cover, R. M. Peek, P. H. Chyou, G. N. Stemmermann and A. Nomura. 1995. Infection with Helicobacter pylori strains possessing Cag A associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 55, 2111-2115
  4. Covacci, A., S. Censini, M. Bugnoli, R. Petracca, D. Burroni, G. Macchia, A. Massone, E. Papini, Z. Xiang and N. Figura. 1993. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA 90, 5791-5795
  5. Cover, T. L. and Blaser, M J. 1992. Purification and characterization of the vacuolation toxin from Helicobacter pylori. J. Biol. Chem. 267, 10570-10575
  6. Crabtree, J. E., S. M. Farmery, I. J. Lindley, N. Figura, P. Peichl and D. S. Tompkins. 1994. CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines. J. Clin. Pathol. 47, 945-950 https://doi.org/10.1136/jcp.47.10.945
  7. Crabtree, J. E., P. Peichl, J. I. Wyatt, U. Stachl and I. J. Lindly. 1993. Gastric interleukin-8 and IgA 1L-8 autoantibodies in Helicobacter pylori infection. Scand. J. Immunol. 37, 65-70 https://doi.org/10.1111/j.1365-3083.1993.tb01666.x
  8. Crabtree, J. E., T. M. Shallcross, R. V. Heatley and J. I. Wyatt. 1991. Mucosal tumour necrosis factor alpha and interleukin-6 in patients with Helicobacter pylori associated gastritis. Gut 32, 1473-1477 https://doi.org/10.1136/gut.32.12.1473
  9. Crabtree, J. E., J. I. Wyatt, L. K. Trejdosiewicz, P. Peichl, P. H. Nichols, N. Ramsay, J. N. Primrose and I. J. Lindly. 1994. Interleukin-8 expression in Helicobacter pylori infected, normal, and neoplastic gastroduodenal mucosa. J. Clin. Pathol. 47, 61-66 https://doi.org/10.1136/jcp.47.1.61
  10. Eckmann, L., M. F. Kagnoff and J. Fierer. 1993. Epithelial cells secrete the chemokine interleukin-8 in response to bacterial entry. Infect. Immun. 61, 4569-4574
  11. Gionchetti, P., D. Vaira, M. Campieri, J. Holton, M. Menegatti, A. Belluzzi, E. Bertinelli, M. Ferretti, C. Brignolac and M. Miglioli. 1994. Enhanced mucosal interleukin-6 and -8 in Helicobacter pylori-positive dyspeptic patients. Am. J. Gastroenterol. 89, 883-887
  12. Huang, J., P. W. O'toole, P. Doig, and T. J. Trust. 1995. Simulaltion of Interleukin-8 Production in Epithelial Cell Lines by Helieobacter pylori. Infect, Immun. 63, 1732-1738
  13. Lee, A., J. Fox and S. Hazell. 1993. Pathogenicity of Helicobacter pylori: a perspective. Infect. Immun. 61, 1601-1610
  14. Nakajima, N., H. Kuwayama, N. Tanaka, M. Nakajima and G. L. Eastwood. 1990. Campylobacter pylori filtrate inhibits human epithelial growth factor-stimulated gastric epithelial proliferation in vitro. Gastroenterology 98, A94
  15. Noach, J. A., N. B. Bosma, J. Jansen, F. J. Hoek, S. J. H. van Deventer and G. N. J. Tytgat. 1994. Mucosal tumor necrosis factor-${\alpha}$, interleukin-1${\beta}$, and interleukin-8 production in patients with Helicobacter pylori infection. Seand. J. Gastroenterol. 29, 425-429 https://doi.org/10.3109/00365529409096833
  16. Nomura, A, G. N. Stemmermann, P. H. Chyou, I. Kato, G. I. Perez-Perez and M. J. Blaser. 1991. Helieobacter pylori infection and gastric carcinoma in a population of Japanese Americans in Hawaii. N. Engl. J. Med. 325, 1132-1136 https://doi.org/10.1056/NEJM199110173251604
  17. Oppenheim, J. J., C. O. C. Zachariae, N. Mukaiada and K. Matsushima. 1991. Properties of the novel proinflammatory supergene intercrine cytokine family. Annu. Rev. Immunol. 9, 617-648 https://doi.org/10.1146/annurev.iy.09.040191.003153
  18. Scheynius, A and L. Engstrand. 1991. Gastric epithelial cells in Helicobacter pylori-associated gastritis expressed HLA-DR but not ICAM-1. Seand. J. Immunol. 33, 237-241 https://doi.org/10.1111/j.1365-3083.1991.tb03755.x
  19. Shon, Y. H., J. I. Surh, Y. J. Chung, I. K. Park, H. C. Kim, C. W. Hwang, C. H. Kim and K. S. Nam. 2004. Effect of radish on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Kor. J. Pharmacogn. 35, 250-254
  20. Wagner, S., W. Beil, U. E. H. Mai, M. Lohmuller and M. Manns. 1994. Cocultivation of human gastric epithelial cells and H. pylori: an in vitro model for the initial step in H. pylori infection. Gastroenterology 106, A791
  21. Wyle, F. A., A. Tarnawski, D. Schulman and W. Dabros. 1990. Evidence for gastric mucosal cell invasion by Campylobacter pylori: an ultrastructural study. J. Clin. Gastroenterol. 12, S92-98 https://doi.org/10.1097/00004836-199001001-00016

피인용 문헌

  1. Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab vol.23, pp.2, 2013, https://doi.org/10.5352/JLS.2013.23.2.213