KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Integration of immunohistochemical reactions into Electrochemical and Optical Analyses of Biochips

면역 조직화학 반응이 통합된 바이오칩의 전기화학 및 광학적 분석

  • Choi Hyoung Gil (Department of Biotechnology, Ajou University) ;
  • Hong Eun Kyoung (Department of Anatomy, Chonnam National University Medical School) ;
  • Lee Seung-Won (Department of Anatomy, Chonnam National University Medical School) ;
  • Yoon Hyun C. (Department of Biotechnology, Ajou University)
  • 최형길 (아주대학교 생명공학과) ;
  • 홍은경 (전남대학교 의과대학 해부학교실) ;
  • 이승원 (전남대학교 의과대학 해부학교실) ;
  • 윤현철 (아주대학교 생명공학과)
  • Published : 2005.04.01
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Abstract

We have addressed two important issues of immunosensing biochips, including the construction of antibody functionalized suface for efficient affinity reactions and the development of a signal registration strategy that converts biospecific reactions into highly quantifiable electrochemical and/or optical signals. The developed immunoassay reaction is an integrated version of enzyme-mediated immunoprecipitaion reaction, which is widely used in immunohistochemistry, and electrochemical signaling reaction. For the evaluation of analytical performance of fabricated immunosensing biochips, signaling for mouse IgG in antiserum was conducted. Applications of the developed strategy have been found for the evaluation of histology chemicals and for the signal amplification for array-type biochip analysis.

효율적인 바이오칩을 개발을 위해 칩 표면에 생체 물질들의 상호반응이 효과적으로 일어날 수 있는 센싱 표면의 조성과 항원-항체 반응과 같은 생체인식 반응을 정량적 신호로 전환하는 방법에 대해 연구하였다. 전기화학식 센서의 표면을 개선하기 위해 폴리아미도아민 덴드리머를 가교 물질로 도입하였다. 생체 분자들의 인식작용을 정량적인 신호로 전환하기 위해 전형적인 면역조직화학분석에서 사용된 반응들을 바이오센서에 적용한 방법론을 사용하였다 효소에 의해 촉매되는 신호화 방법은 면역반응들에 대하여 광학식 센서와 전기화학식 센서에서 공히 수행되었으며, 매우 정량적인 신호로 측정되었다. 측정된 신호들로부터 단백질 농도에 비례하는 검량곡선을 획득할 수 있었으며 다양한 면역 샘플에 대한 적용 가능성을 제시하였다.

Keywords

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