Development of Mathematical Model for the Hydrolysis Fish Oil

물고기 기름의 가수분해에 대한 수학적 모형개발

  • Kim Won-Ho (Department of Biological and Chemical Engineering, College of Engineering, Inha University) ;
  • Lee Yong-Hoon (Department of Biological and Chemical Engineering, College of Engineering, Inha University) ;
  • Park Ji-Suk (Department of Biological and Chemical Engineering, College of Engineering, Inha University) ;
  • Hur Byung-Ki (Department of Biological and Chemical Engineering, College of Engineering, Inha University)
  • 김원호 (인하대학교 공과대학 생명화학공학부) ;
  • 이용훈 (인하대학교 공과대학 생명화학공학부) ;
  • 박지숙 (인하대학교 공과대학 생명화학공학부) ;
  • 허병기 (인하대학교 공과대학 생명화학공학부)
  • Published : 2005.04.01

Abstract

The functional relationship between the number of mole of an i-fatty acid (Si) included in fish oil and the hydrolysis time(t) was expressed as a mathematical model, $S_i=-{\alpha_i}1n(t)+\beta_i$. The average errors of calculated values on the basis of the measured values were distributed in the range of less than $5\%$ for all the 15 fatty aids composing of fish oil. The equation of hydrolysis rate of each fatty acid was deduced as $v_i={\gamma_i}exp(\frac{S_i}{\alpha_i})$ from the above-mentioned $S_i=-{\alpha_i}ln(t)+{\beta_i}$. Therefore the hydrolysis yields of fatty acids were analyzed using the equation of $S_i\;Vs.\;t.$. The 15 fatty acids were categorized into 4groups from the view point of hydrolysis yield. The hydrolysis yields of the first group, including C14:0, C16:0, C16:1, C18:0, C18:1 (n-7) and 1l8:1 (n-9), were higher than $70\%$ at 48 hr of hydrolysis. Those of the second group, C20:1, C22:1, C18:3, C20:4 and C20:5, were distributed from $40\%,\;to\;60\%$, and third group were around $30\%$. The final group containing only C22:6 was very hard to be hydrolyzed and the yield was less than $20\%$ at the same time.

물고기 기름을 구성하고 있는 15종류 지방산에 대한 mol수와 가수분해 시간 사이의 함수관계를 대수함수식, $S_i=-{\alpha_i}1n(t)+{\beta_i}$로 나타내었다. 동일 가수분해 시간에서 각 지방산에 대한 대수함수식의 $S_i$ 값과 실측치 사이의 오차가 15 종류 지방산 모두에 대하여 $5\%$ 이내에 분포되었다. 각 지방산의 mol수 $S_i$와 가수분해 시간 사이의 대수함수식으로부터 각 지방산에 대한 가수분해 반응속도를 지방산 mol 수의 지수함수 관계식, $v_i={\gamma_i}exp(\frac{S_i}{\alpha_i})$로 유도하였다. 또한 유도된 $S_i$와 t 사이의 함수관계식으로부터 물고기 기름을 구성하는 15 종류 지방산 각각에 대한 가수분해율을 분석하였다. 가수분해 시간 48시간에서 가수분해율이 $70\%$ 이상인 지방산은 C14:0, C16:0, C18:0, C16:1, C18:1(n-7) 및 Cl8:1(n-9)이였으며, 가수분해율이 $40\%$ 내지 $60\%$ 사이에 분포되어 있는 지방산은 C20:1, C22:1, C18:3, C18:4, C20:4 및 C20:5이였으며, 가수분해율이 $30\%$ 내외인 지방산은 C2l:5와 C22:5이였으며, $20\%$ 이하인 지방산은 C22:6이였다.

Keywords

References

  1. Yukihisa Tanaka, Jiro Hinaro, and Tadushi Funda (1993), Concentration of Docosahexaenoic Acid in Glyceride by Hydrolysis of Fish Oil with Candida cylindracea Lipase, J. Am. Oil Chem. Soc. 36, 5797-5794
  2. Shimada, Y., K. Marugama, S. Okazaki, M. Nakamura, A. Sugihara, and Y. Tomina (1994), Enrichment of Polyunsaturated Fatty Acids with Geotrichum candidum Lipase, J. Am. Oil Chem.Soc. 71, 951-954 https://doi.org/10.1007/BF02542260
  3. Jin, Y. S. and B. K. Hur (1998), Process Development of Concentration of n-3 PuFAs from Fish Oil by means of Lipase, Korean J. Biotechnol. Bioeng. 13, 90-95
  4. Hur, B. K., D. J. Woo and C. B. Kim (1999), Hydrolysis Mechanisms of Fish Oil by Lipolase-100T, J. Mierobiol. Biotechnol., 9(5), 624-630
  5. Okumura, S., M. Zwai, and Y. Tsujisaka (1980), Purification and properties of Partial Glyceride Hydrolase of Penicillium cyclopim M1, J. Biochel. 87, 205-211 https://doi.org/10.1093/oxfordjournals.jbchem.a132726
  6. Boswinkel, G., J. T. P. Derken, K. van't Riet, and F. P. Cuperusa (1996), Kinetics of Acyl Migration in Monoglycerides and Dependence on Acyl Chain Length, J. Am. Oil Chem. Soc. 73(6), 707-711 https://doi.org/10.1007/BF02517944
  7. Yadwad, V. B., O. P. Ward, and L. C. Noronha (1991), Application of Lipase to Concentrate the Docosahexaenoic Acid(DHA) Fration of Fish Oil, Biotechnol. Bioeng. 38, 956-959 https://doi.org/10.1002/bit.260380818
  8. Li, Z. Y. and O. P. Ward (1993), Enzyme Catalyzed Production of Vegetable Oils Containing Omega-3 Polyunsaturated Fatty Acid, Biotechnol. Lett. 15, 185-188 https://doi.org/10.1007/BF00133021
  9. Mukherjee, K. D., I. Kiewitt, and M. J. Hills (1993), Substrate Specificies of Lipases in View of Kinetic Resolution of Unsaturated Fatty Acids, Appl. Mierobiol. Biotechnol. 40, 489-493
  10. Bailey, J. E. and David, F. O. (1986), Biochemical Engineering Fundamentals, 2nd ed. McGow-Hill, 100
  11. 서정윤 (1998) 제4장 복수기질반응, 75-99, 믿음사
  12. 한국공업규격 시료유지의 탈산방법(1985). KSM 2731
  13. Lepage, G. and C. C. Roy (1984), Improved Recovery of Fatty Acid through Direct Transesterification without Prior Extraction or Purification, J. Lipid Res. 25, 1391-1396