Journal of Microbiology and Biotechnology

  • 제15권4호
  • /
  • Pages.787-791
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  • 2005
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Large-Scale Fermentation for the Production of Teicoplanin From a Mutant of Actinoplanes teichomyceticus

  • LEE JAE-CHAN (Korea Research Institute of Bioscience & Biotechnology) ;
  • MIN JUNG-WON (Korea Research Institute of Bioscience & Biotechnology) ;
  • PARK DONG-JIN (Korea Research Institute of Bioscience & Biotechnology) ;
  • SON KWANG HEE (Korea Research Institute of Bioscience & Biotechnology) ;
  • YOON KI-HONG (School of Food Science & Biotechnology, Woosong University) ;
  • PARK HAE-RYONG (Division of Food Science and Biotechnology, Kyungnam University) ;
  • PARK YOUNG-SOO (Central Research Center, Kunwha Pharmaceutical Co.) ;
  • KWON MU-GIL (Central Research Center, Kunwha Pharmaceutical Co.) ;
  • LEE JUNG-MIN (Korea Research Institute of Bioscience & Biotechnology) ;
  • KIM CHANG-JIN (Korea Research Institute of Bioscience & Biotechnology)
  • 발행 : 2005.08.01
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초록

Mutation and its pilot-scale fermentation were conducted for the production of teicoplanin from Actinoplanes teichomyceticus. The fermentation medium was optimized by replacement and Plackett-Burman experimental design. A maximum production of 1,500 mg/l teicoplanin was obtained by pilot-scale fermentation in an optimized medium containing (g/l): 30 g maltodextrin, 5 g glucose, 5 g yeast extract, 5 g soybean meal, 0.5 g $MgSO_4{\cdot}7H_2O$, 0.1 g NaCl, 0.1 g $CaCl_2{\cdot}2H_2O$, and 50 g Diaion HP-20. The production of teicoplanin was improved 3-fold from the parental strain by mutation, media optimization, and fermentation, and laboratory-scale fermentation was successfully demonstrated in a pilot-scale fermenter for the industrial production of teicoplanin.

키워드

참고문헌

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