Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Characterization of CDMHK, a Growth Inhibitory Molecule Against Cancer Cell Lines, from Myxobacterium sp. HK1 Isolated from Korean Soil

  • LEE HAN-KI (Division of Bioscience and Bioinformatics, Myongji University) ;
  • LEE IN-HYE (Division of Bioscience and Bioinformatics, Myongji University) ;
  • YIM JEE-SUN (Division of Bioscience and Bioinformatics, Myongji University) ;
  • KIM YONG-HO (Division of Bioscience and Bioinformatics, Myongji University) ;
  • LEE SANG-HEE (Division of Bioscience and Bioinformatics, Myongji University) ;
  • LEE KISAY (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • KOO YOON-MO (Department of Biological Engineering, Inha University) ;
  • KIM SANG-JIN (Microbiology Laboratory, Korea Ocean Research and Development Institute) ;
  • JEONG BYEONG-CHUL (Division of Bioscience and Bioinformatics, Myongji University)
  • Published : 2005.08.01
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Abstract

Myxobacterium sp. HK1, isolated from Korean soil, degrades cellulose, differentiates to fruiting body, and its 16s rDNA has $95\%$ similarity to Polyangium sp. An anticancer molecule, CDMHK, was identified from culture broth of Myxobacterium sp. HK1, and purified by Diaion HP20, Silica gel, Sephadex LH-20 chromatography, and preparative HPLC using an YMC OSD-A C18 column. The molecular structure and formula were determined to be $C_{l2}H_{l9}N_3O_2$ (M.W 237) by MS spectrometry, 300 MHz $^{1}H\;and\;^{13}C$ NMR. The CDMHK was not active against Escherichia coli, Staphylococcus aureus, and Candida albicans. However, this molecule inhibited the growth of various cancer cell lines. The $ED_{50}$ values of CDMHK were determined to be 0.147, 0.086, 0.18, 0.166, and 0.142 $\mu$g/ml against A549, SK-OV-3, SK-MEL-2, VF498, and HCTl5 cancer cell lines, respectively. In addition, the CDMHK was able to induce apoptosis of the CCRF-CEM cancer cell line, evidenced by DNA fragmentation assay and DAPI staining.

Keywords

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