한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제33권2호
  • /
  • Pages.90-95
  • /
  • 2005
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

반코마이신 내성 Staphylococcus aureus 억제 물질 생산 Streptomyces sp.의 분리 및 동정

Isolation and Identification of Streptomyces sp. Producing Anti-vancomycin Resistant Staphylococcus aureus Substance

  • 오세택 (건국대학교 생명공학과) ;
  • 이준재 (건국대학교 생명공학과) ;
  • 이지연 (건국대학교 생명공학과) ;
  • 김진규 (건국대학교 생명공학과) ;
  • 양시용 (건국대학교 생명공학과) ;
  • 김양수 (울산의과대학교 서울아산병원 감염내과) ;
  • 송민동 (건국대학교 생명공학과)
  • Oh Se-Teak (Department of Biotechnology, Konkuk University) ;
  • Lee Jun-Jae (Department of Biotechnology, Konkuk University) ;
  • Lee Ji-Youn (Department of Biotechnology, Konkuk University) ;
  • Kim Jin-Kyu (Department of Biotechnology, Konkuk University) ;
  • Yang Si-Yong (Department of Biotechnology, Konkuk University) ;
  • Kim Yang-Soo (Division of Infectious Diseases, Seoul Asan Medical Center, University of Ulsan College of Medicine) ;
  • Song Min-Dong (Department of Biotechnology, Konkuk University)
  • 발행 : 2005.06.01
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초록

An Actinomycetes producing an anti-VRSA (vancomycin-resistant Staphylococcus aureus) substance was isolated from soil. The cultural, morphological, physiological and phylogenetic analyses of an isolated strain were investigated for identification. Cultural characteristics based on ISP (International Streptomyces Project) were as follows: white aerial mycelium, yellow reverse side, and good growth on various medium. Also, the isolate did not produce the soluble pigment. Morphological characteristics were showed cylindrical spore chain and smooth spore surface by SEM (Scanning Electron Microscope). Physiological characteristics were showed LL-type by DAP isomer analysis and detected glycine, glutamic acid and alanine. A phylogenetic analysis of the 16S rDNA provided a clue that the isolated strain was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyces echinatus. The isolate was identified to be a genus of Streptomyces sp.. The optimal culture conditions for the maximum production of anti-VRSA substance by Streptomyces sp. were attained in a culture medium composed of $2.0\%$ (w/v) glucose, and $0.4\%$ (w/v) yeast extract. The anti-VRSA substance was highly produced after 5 days of culture. Optimal pH and temperature conditions for the production of anti-VRSA substance were pH 7.0 and $28^{\circ}C$, respectively.

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