Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparison of Ginseng Saponin, Fatty Acid, Polysaccharide, Phenolic Compound and Organic Acid of Ginseng Hairy Roots and Callus

인삼모상근과 캘루스의 사포닌, 지방산, 산성다당체, 페놀성 물질 및 유기산의 함량비교

  • Lee Jun-Won (Dept. of Oriental Medicinal Materal and Processing, College of Life Science, Kyung Hee University) ;
  • Kim Jin-Ju (Dept. of Oriental Medicinal Materal and Processing, College of Life Science, Kyung Hee University) ;
  • Yang Deok-Chun (Dept. of Oriental Medicinal Materal and Processing, College of Life Science, Kyung Hee University)
  • 이준원 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 김진주 (경희대학교 생명과학대학 한방재료가공학과) ;
  • 양덕춘 (경희대학교 생명과학대학 한방재료가공학과)
  • Published : 2005.06.01
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Abstract

Korean ginseng(Panax ginseng C.A. Meyer) roots have long been known as the best medicinal plant and its pharmaceutical bio-activities have been proven by scientific analyses of their components - ginsenosides, acidic polysaccharides, phenolic compounds, fatty acids and organic acids etc. Ginseng hairy roots and callus have been cultured in vitro for stable supply of ginseng material. In this study, the amount of ginsenosides, fatty acids, acidic polysaccharides, phenolic compounds and organic acids in ginseng hairy roots and callus were compared. Higher amount of ginsenoside was found in ginseng hairy roots than ginseng callus. Higher amount of saturated fatty acid (palmitic acid) was found in callus and higher amount of unsaturated fatty acid (linoleic acid) was found in hairy roots. Acidic polysaccharide and phenolic compounds were contained by the same amount in both hairy roots and callus. Organic acids were found more in hairy roots.

인삼원료삼의 안정적인 공급을 위하여 인삼의 기내배양에 의하여 생산된 캘루스 및 모상근으로부터 인삼사포닌, 지방산, 산상다당체, 페놀성화합물 및 유기산함량을 조사하였다. 인삼사포닌의 경우에는 캘루스에 비해 모상근에서 훨씬 많았으며 모상근 세포주간에는 약 10mg/g로 유사한 경향을 보였다. 포화지방산은 인삼 캘루스에서 높은 반면 모상근의 경우에는 불포화지방산이 오히려 많거나 비슷한 경향을 보였다. 특히 인삼 캘루스에서는 palmitic acid가 많이 존재하였으며, 인삼 모상근의 경우에는 불포화지방산인 linoleic acid가 캘루스의 5배 이상 많은 검출되었다. 산성 다당체와 페놀성화합물은 기내 배양한 인삼 캘루스 조직에서는 거의 동량으로 존재하였으나, 인삼 모상근의 경우에는 세포주에 따라서 많은 차이를 나타내었다. 특히 HR-2같은 경우에는 산성 다당체보다는 페놀화합물이 $2.26\%$로 산성 다당체보다 2배 이상 많이 존재하였는데 반하여, HR-3 인삼 모상근의 경우에는 산성 다당체가 $1.64\%$로 페놀화합물보다 4배 이상 존재하는 것으로 분석되었다. 인삼 모상근세포주와 캘루스의 유기산은 모상근 세포주가 34.64 mg으로 가장 많이 존재하는 것으로 조사되었다.

Keywords

References

  1. Ando, T., O. Tanaka and S. Shibata. 1971. Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Syoyakugaku Zasshi. 25 : 28-32
  2. Furuya, T. 1981. Plant tissue culture of Korean ginseng. In Recent Studies on ginseng. pp. 67-72
  3. Furuya, T., T. Yoshikawa, T. Ishii and K. Kaji. 1983a. Effects of auxins on growth and saponin production in callus cultures of Panax ginseng. Planta Medica 47 : 183-18 https://doi.org/10.1055/s-2007-969981
  4. Furuya, T., T. Yoshikawa, Y. Orihara and H. Oda. 1983b. Saponin Production in Callus Suspension Cultures of Panax ginseng. Planta Med. 48 : 83-87 https://doi.org/10.1055/s-2007-969892
  5. Hamill, J.D., A.J. Parr, R.J. Robins and M.J.C. Rhodes. 1986. Secondary product formation by cultures of Beta vulqaris and Nicotiana rustica transformed with Aqrobacterium rhizogenes. Plant Cell Reports. 5 : 111-114 https://doi.org/10.1007/BF00269247
  6. Han, B.H, M.H. Park, Y.N. Han and S.C. Shin. 1984. Studies on the antioxidant components of Korean ginseng-(IV)-antifatigue activity components. Yakhakhoeji, 28 : 232-235
  7. Huh, B.H, I.R. Lee and B.H. Han. 1990. Lignans from Korean red ginseng. Arch Pharm Res 13 : 278-81 https://doi.org/10.1007/BF02856536
  8. Hwang, W.I. and S.K. Oh. 1984. A study on the anticancer activities of lipid soluble ginseng extract and ginseng saponin derivatives against some cancer cells. Korean J. Ginseng Sci. 8 : 153-159
  9. Hwang, W.I., K.H. Park and J.M. Paik. 1987. A cytotoxic activity of Panax ginseng extract against some cancer cells in vivo and in vitro. Korean J. Ginseng Sci. 11 : 173-179
  10. Kwon, B.M., S.H. Ro, J.Y. Nam, H.J. Jung, I.R. Lee and Y.K. Kim. 1997. Poly acetylene analogs, isolated from hairy roots of Panax ginseng, inhibit Acyl-CoA : cholesterol acetyltransferase. Planta Med 63 : 552-553
  11. Matsunaga, H., M. Katano, H. Yamamoto, H. Fujito and M. Mori. 1990. Cytotoxic activity of poly acetylene compounds in Panax ginseng C.A. Meyer. Chem Pharm Bull 38 : 3480-3482 https://doi.org/10.1248/cpb.38.3480
  12. Osamu, T. 1977. Recent studies on the chemistry of ginseng saponins. Korea J. Ginseng Sci. 2(1) : 9-15
  13. Park, H.J., S.Y. Oh., K.H. Choi., S.J. Meang, E.S. Yoon. and D.C. Yang. 2000. Effects of jasmonic acid and methyl jasmonate on the production of ginsenosides in the hairy roots of Korean Ginseng (Panax ginseng CA. Meyer). J. Ginseng Res. 24 : 74-78
  14. Shin, S.C., H.Y. Koh and B.H. Han. 1983. Polyacetylenes from Panax ginseng roots. Phytochemistry 22(8) : 1817-1818 https://doi.org/10.1016/S0031-9422(00)80278-2
  15. Yang, D.C, H.K. Kwon, H.J. Park, B.H. Min, N.H. Song and K.T. Choi. 2000. Selection of cell lines for high yields of antioxidants from callus of ginseng superior lines. J Ginseng Res 24 : 157-161
  16. Yang, D.C. and K.J. Yang. 2000. Patterns and contents of ginsenoside in normal root parts and hairy root lines of Panax ginseng C.A. Meyer. Korean J. Plant Tissue Culture 27 : 485-489
  17. Yang, D.C., N.H. Song, K.J. Yang and C.H. Bae. 2001. Analysis and culture conditions for biosynthesis of polyacetylene from callus of ginseng superior lines. Korean J. Plant Tissue Culture 28(3) : 123-128
  18. Yoo, B.S., H.J. Lee, S.R. Ko, D.C. Yang and S.Y. Byun. 2000. Studies on the extraction of polyacetylee from Korean ginseng using supercritical carbon dioxide. Korean J. Biotechnol Bioen. 15 : 80-83
  19. Yoo, B.S. and S.Y. Byun. 2001. Characterization of batch culture and effect of the various elicitors on ginsenoside production in suspension cultures of Panax ginseng C. A. Meyer. Korean K. Biotechnol. Bioeng. 16 : 620-625
  20. Yoshikawa, T. and T. Furuya. 1987. Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep. 6 : 449-453
  21. Yun, Y.S, S.K. Jo, H.S. Moon, YJ. Kim, Y.R. Dh and T.K. Yun. 1985. Effect of red ginseng on natural killer cell activity in mice with lung adenoma induced by urethan and benzo(a)pyrene. Korean Biochem J 18 : 31-37
  22. 김만욱. 1989. 인삼 연구보고서 효능분야 157-196. 한국인삼연초연구원
  23. 김만욱. 1990. 인삼 연구보고서 효능분야 148-189. 한국인삼연초연구원
  24. 남기열. 1996. 최신고려인삼(성분과 효능편). 천일인쇄사, 서울. pp. 247-252