Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Peptide Inhibitor for Angiotensin-Converting Enzyme from Thermolysin Hydrolysate of Manila Clam Proteins

  • Lee Tae-Gee (Department of Hotel Culinary & Baking, Namdo Provincial College of Jeonnam) ;
  • Yeum Dong-Min (Department of Food Processing & Baking, Yangsan College) ;
  • Kim Young-Sook (Department of Food Processing & Baking, Yangsan College) ;
  • Yeo Saeng-Gyu (Department of Hotel Culinary Arts, Busan College of Information Technology) ;
  • Lee Yong-Woo (Division of Food Science, Dongeui Institute of Technology) ;
  • Kim Jin-Soo (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University) ;
  • Kim In-Soo (Division of Marine Bioscience Institute of Marine Industry, Gyeongsang National University) ;
  • Kim Seon-Bong (Faculty of Food and Biotechnology, Pukyong National University)
  • Published : 2005.06.01
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Abstract

A peptide that inhibits angiotensin-converting enzyme (ACE) was isolated from a hydrolysate of Manila clam (Ruditapes philippinarum) proteins prepared with thermolysin. Amino acid sequence of the peptide was determined to be Leu-Leu-Pro. Chemically synthesized Leu-Leu-Pro had an $IC_{50}\;value\;of\;158\;\mu{M}$. Peptides related to the Manila clam-derived peptide were synthesized to study the structure-activity relationships. The tetrapeptide, Leu-Leu-Pro-Pro, had a very weak effect on the enzyme. However, Leu-Leu-Pro-Asn showed no inhibitory activity.

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References

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