한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)

  • 제38권3호
  • /
  • Pages.148-152
  • /
  • 2005
  • /
  • 0374-8111(pISSN)
  • /
  • 2287-8815(eISSN)
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)
권호 표지
DOI QR코드

DOI QR Code

별불가사리(Asterina pectinifera)로부터 새로운 평활근 이완활성 펩타이드의 정제

Purification and Characterization of a Novel Neuvopeptide with a Smooth Muscle-Relaxing Activity from the Starfish, Asterina pectinifera

  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 안상현 (부경대학교 수산과학대학 생물공학과) ;
  • 손희영 (부경대학교 수산과학대학 생물공학과) ;
  • 박희연 (국립수산과학원 생명공학연구단) ;
  • 윤호동 (국립수산과학원 생명공학연구단) ;
  • 장영채 (대구가톨릭대학교 의과대학) ;
  • 홍용기 (부경대학교 수산과학대학 생물공학과) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • GO Hye-Jin (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • KIM In Hae (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • AN Sang Hyun (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • SOHN Hee-Young (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Hee Yeon (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • YOON Ho Dong (Biotechnology Research, National Fisheries Research & Development Institute) ;
  • CHANG Young-Chae (Department of Pathology, School of Medicine, Catholic University of Daegu) ;
  • HONG Yong-Ki (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, Faculty of Food Science and Biotechnology, Pukyong National University)
  • 발행 : 2005.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A novel neuropeptide with a relaxing activity on the dorsal retractor muscle (DRM) was isolated from the whole body extract of the starfish, Asterina pectinifera. The peptide was purified by gel-filtration ion-exchange and $C_{18}$ reversed-phase HPLC. The complete amino acid sequence of this peptide, which was determined by automated Edman degradation and MALDI-TOF mass, was Phe-Gly-Lys-Gly-Gly-Ala-Tyr-Asp-Pro-Leu-Ser-Ala-Gly-Phe-Thr-Asp. A comparison of the amino acid sequence with those of other known neuropeptides revealed that the asteripectin was a novel neuropeptide with smooth muscle-relaxing activity on the starfish DRM. This peptide showed threshold response to relaxing activity on the DRM at $10^{-10}M$ and the maximal relaxing effect was $120{\pn}7.0\%$ at $10^{-5}M$. The relaxing activity of this peptide on the starfish DRM increased in a dose-dependent manner.

키워드

참고문헌

  1. Diaz-Miranda, L., R.E. Blanco and J.E. Garcia-Arraras. 1995. Localization of the heptapeptide GFSKLYF-AKLYFamide (Echinodermate): A light and electron microscopic study. J. Comp. Neurol., 352, 626-640 https://doi.org/10.1002/cne.903520410
  2. Elphick, M.R. and R. Melarange. 2001. Neural control of muscle relaxation in echinoderms. J. Exp. Biol., 204, 875-885
  3. Elphick, M.R., D.A. Price, T.D. Lee and M.C. Thorndyke. 1991. The SALMFamides: A new family of neuropeptides in the starfish Asterias rubens. J. Exp. Biol., 198, 2519-2525
  4. Iwakoshi, E., M. Ohtani, T. Takahashi, Y. Muneoka, T. Ikeda, T. Fujita, H. Minakata and K. Nomoto. 1995. Comparative aspects of structure and action of bioactive peptides isolated from the sea cucumber Stich opus japonicus. In: Peptide Chemistry, M. Ohno ed. Osaka: Protein Research Foundation, 1994, pp. 261-264
  5. Moore, S.J. and M.C. Thorndyke. 1993. Immunocytochemical mapping of the novel echinoderm neuropeptide SALMFamide 1 (S1) in the starfish, Asterias rubens. Cell Tissue Res., 274, 602-618
  6. Motokawa, T. 1984. Connective tissue catch in echinoderms. Biol. Rev., 59, 255-270 https://doi.org/10.1111/j.1469-185X.1984.tb00409.x
  7. Newman, S.J., M.R. Elphick and M.C. Thorndyke. 1995a. Tissue distribution of the SALMFamide neuropeptides S1 and S2 in the starfish Asterias rubens using novel monoclonal and polyclonal antibodies. I. Nervous and locomotory system. Pro. R. Soc. Lond. B, 261, 139-145
  8. Newman, S.J., M.R. Elphick and M.C. Thorndyke. 1995b. Tissue distribution of the SALMFamide neuropeptides S1 and S2 in the starfish Asterias rubens using novel monoclonal and polyclonal antibodies. II. Digestive system. Proc. R. Soc. Lond. B, 216, 187-192
  9. Park, N.G., J.K. Seo, H.J. Ku, S. Lee, G. Sugihara, K.H. Kim, J.S. Park and S.W. Kang. 1997. Conformation and biological activity of mastoparan B and its analogs I. Bull. Kor. Chem. Soc., 18, 50-56.
  10. Twarog, B.M. 1967. The regulation of catch in molluscan muscle. J. Gen. Physiol., 50, 157-169 https://doi.org/10.1085/jgp.50.6.157

피인용 문헌

  1. 별불가사리 추출물의 면역세포 활성화 효과 vol.36, pp.3, 2007, https://doi.org/10.3746/jkfn.2007.36.3.269
  2. Marine natural products vol.24, pp.1, 2007, https://doi.org/10.1039/b603047p