Journal of Nutrition and Health

  • 제38권5호
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  • Pages.352-363
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  • 2005
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

고지방고콜레스테롤식이를 섭취한 흰쥐에서 식물성식품의 혼합물 첨가로 인한 체내 항산화능 증가 효과

Antioxidative Effect of Plant Food Mixtures in Rat Fed on High Fat-High Cholesterol Diet

  • 곽충실 (서울대학교 의학연구원 체력과학노화연구소) ;
  • 김미연 (이화여자대학교 식품영양학과) ;
  • 이미숙 (한남대학교 식품영양학과)
  • Kwak, Chung-Shil (Aging and Physical Culture Research Institute, Medical college of Seoul National University) ;
  • Kim, Mee-Yeon (Department of Food and Nutrition, Ewha Woman's University) ;
  • Lee, Mee-Sook (Department of Food and Nutrition, Hannam University)
  • 발행 : 2005.06.01
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초록

The previous extensive in vitro studies on the antioxidative activities of a number of Korean grains, vegetables, seaweeds and mushrooms, and the various combinations of these food source exhibited a wide range of antioxidative activities, and four food mixtures composed of 5 kinds of foods (5A, 5B, 5C and 5D) were designed from 16 selective foods showing. high antioxidant effect, in vitro, to find the good combinations for the meal planning. Mixture 5B or 5C contained very high levels of total flavonoid and polyphenol, and ethanol extract from 5A, 5B or 5C showed very strong inhibitory effects against in vitro $Fe^{2+}-induced$ lipid peroxidation and ethanol extract from 5B or 5C showed remarkable DPPH radical scavenging effect and lipid peroxide-protein conjugation inhibition effect. And in vivo study was also carried out with two mixtures (5B, 5C). Powders (P5B, P5C) or ethanol extracts (E5B, E5C) of these mixtures were supplemented to Sprague-Dawley rats fed on high fat $(15\%)-high$ cholesterol $(1\%)$ semipurified diet for 5 weeks. The total antioxidant power in serum was significantly higher in P5B, P5C, E5B and E5C groups than in high fat control group, and $ascorbate-Fe^{2+}-induced$ TBARS was significantly lowered by E5B supplementation in rat liver. In liver tissue, Cu, Zn-SOD activity was significantly higher in P5B and E5B groups than in high fat control group, while catalase or GSH-peroxidase (GPx) activity was not changed by any supplementations. In kidney, Cu, Zn­SOD activity was significantly higher in P5B group than in high fat control group, while GPx activity was not changed by any supplementations. Taken together, mixture 5B and 5C showed very strong antioxidative effects both in vitro and in vivo. Therefore, the ingredient Korean foods of 5B and 5C could be recommended to take a lot together for prevention from age-related chronic diseases.

키워드

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