Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Apoptosis Induced by BARODON® in Human Gastric Cancer Cells

BARODON® 에 의한 Human Gastric Adenocarcinoma AGS 세포고사

  • Jo Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Choi Soo-Il (BARODON-S.F. CORP.) ;
  • Kim Soo-Rim (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn Nam-Shic (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Yang Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park Yong-Ho (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Lee Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • 조은혜 (서울대학교 수의과대학 공중보건학교실) ;
  • 최수일 ;
  • 김수림 (서울대학교 수의과대학 공중보건학교실) ;
  • 조성대 (서울대학교 수의과대학 공중보건학교실) ;
  • 안남식 (서울대학교 수의과대학 공중보건학교실) ;
  • 정지원 (서울대학교 수의과대학 공중보건학교실) ;
  • 양세란 (서울대학교 수의과대학 공중보건학교실) ;
  • 박준석 (서울대학교 수의과대학 공중보건학교실) ;
  • 황재웅 (서울대학교 수의과대학 공중보건학교실) ;
  • 박용호 (서울대학교 수의과대학 세균학교실) ;
  • 이영순 (서울대학교 수의과대학 공중보건학교실) ;
  • 강경선 (서울대학교 수의과대학 공중보건학교실)
  • Published : 2005.06.01
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Abstract

[ $BARODON^{(R)}$ ] is a multi-purpose, high functional alkali solution made by mixing and liquid-ionizing silicon, calcium, sodium, borax, organic carbon chemicals and silver. In this study, we have investigated the apoptotic potential and mechanistic insights of $BARODON^{(R)}$ in human gastric cancer cell line (AGS cells). In MTT assay, $BARODON^{(R)}$ reduced cell viability in AGS cells. Morphological features of apoptosis with marked cytoplasmic vacuolation and appearance of apoptotic peaks in flow cytometry were observed in AGS cells with$BARODON^{(R)}$ treatment. In addition, $BARODON^{(R)}$ induced apoptosis of stomach cancer cell is related to bax up-regulation, caspase 7 protease activation and subsequent cleavage of poly (ADP-ribose) polymerase (PARP). These results suggest that BARODON can induce the apoptosis of AGS cells through modulation of bcl-2 family and the activation of intrinsic caspase cascades, indicating that it is potentially useful as a anti-cancer agent.

Keywords

References

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