Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antioxidative Activity and Irritation Response of Lespedeza bicolor

  • Lee Yang-Suk (Department of herbal Biotechnology, Daegu Haany University) ;
  • Chang Zhiqiang (Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Kyungpook National University) ;
  • Park Seung-Chun (Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Kyungpook National University) ;
  • Rim Nac-Ryong (Division of Science Education, Chonbuk National University) ;
  • Kim Nam-Woo (Department of herbal Biotechnology, Daegu Haany University)
  • Published : 2005.06.01
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Abstract

In the present study, we evaluated the free radical scavenging and xanthine oxidase inhibitory activities exhibited by extracts obtained from the dried stems (and leaves) of Lespedeza bicolor We also assessed its potential irritation activities with regard to cosmetic use. When the DPPH radical scavenging activities of L. bicolor were assessed at six different concentrations (0, 50, 100, 250, 500 and 1000 ${\mu}g/ml$), the concentration of L. bicolor required to inhibit DPPH radical formation by $50\%$ was found to be $164.90{\mu}g/ml$. The effects of L. bicolor on the inhibition of xanthine oxidase were determined at seven different concentrations. The $50\%$ effective concentration was found to be $282.75{\mu}g/ml$. In the skin irritation test, all animals survived for the duration of the study, and all exhibited normal gains in body weight. The control sites exhibited no response to the control procedures. No edema, erythema, or eschar formation was observed in any of the tested rabbits. In the ocular irritation study, all of the rabbit eyes remained normal. In summary, L. bicolor extracts were considered to be non-irritating to the skin and eye.

Keywords

References

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