Reproductive and Developmental Biology

  • 제29권2호
  • /
  • Pages.83-91
  • /
  • 2005
  • /
  • 1738-2432(pISSN)
  • /
  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Characterization of Placental Proteins in Bovine Somatic Cell Clone Fetuses

  • Woo, Jei-Hyun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration, Genetic Engineering Laboratory, Department, Genetic Engineering, Sungkyunkwan University) ;
  • Ko, Yeoung-Gyu (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Kim, Bong-Ki (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Kim, Jong-Mu (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Lee, Youn-Su (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Kim, Nam-Yun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Im, Gi-Sun (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Yang, Boung-Chul (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Seong, Hwan-Hoo (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Jung, Jin-Kwan (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration) ;
  • Kwun, Moo-Sik (Genetic Engineering Laboratory, Department, Genetic Engineering, Sungkyunkwan University) ;
  • Chung, Hak-Jae (Molecular Endocrinolgy Laboratory, Department of Animal Biotechnology, National Livestock Research Institute Rural Development Administration)
  • 발행 : 2005.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Somatic cell nuclear transfer in cattle has limited efficiency in terms of production of live offspring due to high incidence of fetal failure after embryo transfer to recipients. Such low efficiency of cloning could possibly arise from abnormal and poorly developed placenta. In the present study the placental proteome in late pregnancy established from in vitro fertilization (IVF) and nuclear transfer (NT) was analysed. Proteome alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing placenta from NT embryos to those from IVF counterparts, significant changes in expression level were found in 18 proteins. Of these proteins 12 were not expressed in NT placenta but expressed in IVF counterpart, whereas the expression of the other 6 proteins was limited only in NT placenta. Among these proteins, cytokeratin 8 and vimentin are considered to be involved in regulation of post-implantation development. In particular, cytokeratin 8 and vimentin may be used as makers for placental development during pregnancy because their expression levels changed considerably in NT placental tissue compared with its IVF counterpart. Data from 2-DE suggest that protein expression was disorientated in late pregnancy from NT, but this distortion was eliminated with progression of pregnancy. These findings demonstrate abnormal placental development during late pregnancy from NT and suggest that alterations of specific placental protein expression may be involved in abnormal function of placenta.

키워드

참고문헌

  1. Abigail LF (2003): The insulin-like growth factors and feto-placental growth. Placenta 24:803-812 https://doi.org/10.1016/S0143-4004(03)00080-8
  2. An BS, Choi KC, Lee GS, Keung PC, Jeung EB (2004): Complex regulation of calbindin-D (9k) in the mouse placenta and extra-embryonic membrane during midand late pregnancy. Mol Cell Endocrinol 12:39-52
  3. Boquest AC, Grupen CG, Harrison SJ, McIlfatrick SM, Ashman RJ, d'Apice AJF, Nottle MB (2002): Production of cloned pigs from cultured fetal fibroblast cells. Biol Reprod 66:1283-1287 https://doi.org/10.1095/biolreprod66.5.1283
  4. Bozic M, Petronijevic M, Milenkovic S, Atanackovic J, Lazic J, Vicovac Lj (2004): Galectin-l and galectin-3 in the trophoblast of the gestation torphoblastic disease. Placenta nov 25(10):797-802 https://doi.org/10.1016/j.placenta.2004.03.006
  5. Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 7:72:248-54
  6. Brackett BJ, oliphant G (1975): Capacitation of rabbit spermatozoa in vitro. Biol Reprod 12:260-274 https://doi.org/10.1095/biolreprod12.2.260
  7. Baguisi A, Behboodi E, Melican DT, Poolock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW, Echolard Y (1999): Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17:456-461 https://doi.org/10.1038/8632
  8. Boquest AC, Grupen CG, Harrison SJ, Mcllfatrick SM, Ashman RJ, d'Apice AJ, Nottle MB (2002): Production of cloned pigs from cultured fetal fibroblast cells. Biol Reprod 66: 1283-1287 https://doi.org/10.1095/​biolreprod66.5.1283
  9. Cross JC, Werb Z, Fisher SJ (1994): Implantation and the placenta: key pieces of the development puzzle. Science 266:1508-1518 https://doi.org/10.1126/science.7985020
  10. Chesne P, Adenot PG, Viglietta C, Baratte M, Boulanger L, Renard JP (2002): Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat Biotechnol 20: 366-369 https://doi.org/10.1038/nbt0402-366
  11. Duello TM, Byatt JC, Bremel RD (1986): Immunohistochemical localization of placental lactogen in binucleate cells of bovine placentomes. Endocrinology 119: 1351-1355 https://doi.org/10.1210/endo-119-3-1351
  12. Daya D, Sabet L (1991): The use of cytokeratin as a sensitive and reliable marker for trophoblastic tissue. Am J Clin Pathol 95:137-141 https://doi.org/10.1093/ajcp/95.2.137
  13. De Sousa PA, King T, Harkness L, Young LE, Walker SK, Wilmut I (2001): Evaluation of gestational deficiencies in cloned sheep fetuses and placentae. Biol Reprod 65: 23-30 https://doi.org/10.1095/biolreprod65.1.23
  14. Eggan K, Akutsu H, Lorgin J, Jackson-Grusby L, Klemm M, Rideout WM III, Yanagimachi R, Jaenisch R (2001): Hybrid vigor, fetal overgrowth, and viability of mice derived by nuclear cloning and tetraploid embryo complementation. Proc Natl Acad Sci 98:6209-6214 https://doi.org/10.1073/pnas.101118898
  15. Gootwine E (2004): Placental hormones and fetal-placental development. Animal Reproduction Science 82-83:551-556
  16. Frank TL, van der Loop, Giulio Gabbiani, Gaby Kohnen, Frans CS, Ramaekers, Guillaume JJM van Eys (1997): Differentiation of Smooth Muscle Cells in Human Blood Vessels as Defined by Smoothelin, a Novel Marker for the Contractile Phenotype. Arterioscler Thromb Vasc Biol 17(4):665-71 https://doi.org/10.1161/01.ATV.17.4.665
  17. Keefer CL, Keyston R, Lazaris A, Bhatia B, Befin I, Bilodeau AS, Zhou FJ, Kafidi N, Wang B, Baldassarre H, Karatzas CN (2002): Production of cloned goats after nuclear transfer using adult somatic cells. Biol Reprod 66:199-203 https://doi.org/10.1095/biolreprod66.1.199
  18. Gootwine E (2004): Placental hormones and fetal- placental development. Anim Reprod Sci 82-83:551-566
  19. Green JA Xie S, Gan X, Mathialagan N, Beckers J, Roberts RM (2000): Pregnancy-associated bovine and glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biol Reprod 62:1624-1631 https://doi.org/10.1095/biolreprod62.6.1624
  20. Huggett ASG, Nixon DA (1961): Fructose as a component of the foetal blood in several mammalian species. Nature 190:1209
  21. Hastein T, Velle W (1968): Placental aldose reductase activity and foetal blood fructose during bovine pregnancy. J Reprod Fertil 15(1):47-52 https://doi.org/10.1530/jrf.0.0150047
  22. Hastein T, Velle W (1969): Purification and properties of aldose reductases from the placenta and the seminal vesicle of the sheep. 18:178(1):1-10
  23. Hass R, Sohn C (2003): Increased oxidative stress in pre-eclamptic placenta is assocaiated with altered proteasome activity and proteon patterns. Placenta 24: 979-984 https://doi.org/10.1016/S0143-4004(03)00174-7
  24. Heyman Y, Chavatte-Palmer P, DeBourhis D, Camous S, Vignon X, Renard JP (2002): Frequency and occurrence of late-gestationlosses from cattle cloned embryos. Biol Reprod 66:6-13 https://doi.org/10.1095/biolreprod66.1.6
  25. Hasler JF, Henderson WB, Hurtgens PJ, Jin ZQ McCauley AD, Mower SA, Neely B, Shuey LS, Stokes JE, Trimmer SA (1995): Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology 43:141-152 https://doi.org/10.1016/0093-691X(94)00020-U
  26. Hill JR, Roussel AJ, Cibelli JB (1999): Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies). Theriogenology 51:1451-1465 https://doi.org/10.1016/S0093-691X(99)00089-8
  27. Hill JR. Burghardt RC Jones K, Long CR Looney CR, Shin T, Spencer TE, Thompson JA, Winger QA, Westhusin ME (2000): Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses. Biol Reprod 63:1787-1794 https://doi.org/10.1095/biolreprod63.6.1787
  28. Im GS, Yang BS, Yang BC, Chang WK, Yi YJ, Park CS, (2001): Effect of cell cycle stage on the development of embryos produced by cumulus cell nuclear transfer in Hanwoo (Korea cattle). Asian-Ausu J Anim Sci 14: 759-764 https://doi.org/10.5713/ajas.2001.759
  29. Kazuyoshi hashizume, Hiroko ishiwata, Keiichiro kizaki, Osamu yamada, Tom takahashi, Kei imai, Osman V. patel, Satoshi akagi, manabu shimizu, Seiya takahashi, Susumu katsuma, Satoshi shiojima, Akira hirasawa, Gozo tsujimoto, Junichi todoroki, and Yoshiaki izaike (2002): Implantation and placental development in somatic cell clone recipient cows. Cloning and Stem Cells 4(3)
  30. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y (1998): Eight calves cloned from somatic cells of a single adult. Science 282:2095-2098 https://doi.org/10.1126/science.282.5396.2095
  31. Kruip TAM, Den Daas JHG (1997): In vitro produced and cloned embryos: effects on pregnancy, parturition and offspring. Theriogenology 47:43-52 https://doi.org/10.1016/S0093-691X(96)00338-X
  32. Kang YK, Koo DB, Park JS (2001): Aberrant methylation of donor genome in cloned bovine embryos. Nature Genetic 28:173-177 https://doi.org/10.1038/88903
  33. Lim SO, Park SJ, Kim W, Park SG, Kim HI (2002): Proteome analysis of hepatocellular carcinoma. Biochem Biophys Res Commun 291:1031-1037 https://doi.org/10.1006/bbrc.2002.6547
  34. Milosavljevic M, Duello T, Schuler L (1989): In-situ localization of two prolactin-related messenger ribonucleic acids to binucleate cells of bovine placentomes. Endocrinology 125:883-889 https://doi.org/10.1210/endo-125-2-883
  35. Moore T (2001): Genetic conflict, genomic imprinting and establishment of the epigenotype in relation to growth. Reproduction 122:185-193 https://doi.org/10.1530/rep.0.1220185
  36. Nelson PS, Han D, Rochon Y, Corthals GL, Lin B, Monson A, Nguyen V, Franza BR, Plymate SR, Aebersold R, Hood L (2000): Comprehensive analyses of prostate gene expression: convergence of expressed sequence tag databases, transcript profiling and proteomics. Electrophoresis 21:2235-2242 https://doi.org/10.1002/1522-2683(20000601)21:11<2235::AID-ELPS2235>3.0.CO;2-A
  37. Niemann H, Wrenzycki C (2000): Alterations of expression of developmentally important genes in preimplantation bovine embryos by in vitro culture conditions: implications for subsequent development. Theriogenology 53:21-34 https://doi.org/10.1016/S0093-691X(99)00237-X
  38. Osman V. Patel, Osamu Yamada, Keiichiro kizaki, Toru Takahashi, Kei lmai, Seiya Takahashi, Yoshiaki lzaike, Linda A. Schuler, Tshiaki Takezawa and Kazuyoshi Hashizume (2004): Expression of trophoblast cell-specific pregnancy-related genes in somatic cell-cloned bovine preganacies. Biol Reprod 70:1114-1120 https://doi.org/10.1095/biolreprod.103.020198
  39. Ohgane J, Wakayama T, Kogo Y (2001): DNA methylation variation in cloned mice. Genesis 30:45-50 https://doi.org/10.1002/gene.1031
  40. Oakley BR, Kirsch DR, Morris NR (1980): A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 1;105(2):361-3 https://doi.org/10.1016/0003-2697(80)90470-4
  41. Otoi T, Tachikawa S, Kondo S, Kono F, Goto M, Ogano Y, Suzuki T (1993): Effect of trypsin treatment of in vitro fertilized bovine embryos on their subsequent survival and development. J Vet Med Sci 55(2):237-9 https://doi.org/10.1292/jvms.55.237
  42. Plejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH (2000): Cloned pigs producted by nuclear transfer from adult somatic cells. Nature 407:86-90 https://doi.org/10.1038/35024082
  43. Souza PC de, Katz SG (2001): Coexpression of cytokeratin and vimentin in mice trophoblastic giant cells. Tissue & Cell 33(1):40-45 https://doi.org/10.1054/tice.2000.0148
  44. Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L, Buck S, Murphy K, Lyons L, Westhusin ME (2002): A cat cloned by nuclear transplantation. Nature 415-859
  45. Viuff D, Rickords L, Offenberg H, Hyttel P, Avery B, Greve T, Olsaker I, Williams JL, Callesen H, Thomsen PD (1999): A high proportion of bovine blastocysts produced in vitro are mixoploid. Biol Reprod 60:1273-1278 https://doi.org/10.1095/biolreprod60.6.1273
  46. Viuff D, Greve T, AveryB, Hyttel P, Brockhoff PB, Thomsen PD (2000): Chromosome aberrations in vitro produced bovine embryos at days 2-5 post-insemination. Biol Reprod 63:1143-1148 https://doi.org/10.1095/biolreprod63.4.1143
  47. Viuff D, Hendriksen PJM, Vos PL, Dieleman SJ, Bibby BM, Greve T, Hyttel P, Thomsen PD (2001): Chromosomal abnormalities and developmental kinetics in in vivo-developed cattle embryos at days 2 to 5 after ovulation. Biol Reprod 65:204-208 https://doi.org/10.1095/biolreprod65.1.204
  48. Viovac L, Aplin JD (1996): Epithelial-mesenchymal transition during trohpoblast differentiation. Acta Anat. 156:202-216 https://doi.org/10.1159/000147847
  49. Van MH, Russell F, Karl C, Alma B, Bradford WB, Susan JF (2001): Functional proteomics: examining the effects of hypoxia on the cytotrophoblast protein repertoire. Biochemistry 40:4044-4086 https://doi.org/10.1021/bi002236l
  50. Van Wagtendonk-de Leeuw AM, Aerts BJG, Den Daas JHG (1998): Abnormal offspring following in vitro production of bovine pre-implantation embryos; a field study. Theriogenology 49:883-894 https://doi.org/10.1016/S0093-691X(98)00038-7
  51. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813 https://doi.org/10.1038/385810a0
  52. Wilmut I(1998): Cloining for medicine. Sci Am 279:58-63
  53. Wells D, Misica P, Day A, Peterson A, Tervit H (1998): Clining sheep from cultured embryonic cells. Reprod Fertil Dev 10:615-626 https://doi.org/10.1071/RD98046
  54. Wells DN, Misica PM, Tervit HR (1999): Production of cloned calves following nuclear transfer with cultured adult mural granuloas cells. Biol Reprod 60:996-1005 https://doi.org/10.1095/biolreprod60.4.996
  55. Wells D, Misica P, Day A, Peterson A, Tervit H (1999): Cloning sheep from cultured embryonic cells. Reprod Fertil Dev 10:615-626 https://doi.org/10.1071/RD98046
  56. Wall RJ, Kerr DE, Bondioli KR (1997): Transgenic dairy cattle: genetic engineering on a large scale. J Dairy Sci 80:2213-2224 https://doi.org/10.3168/jds.S0022-0302(97)76170-8
  57. Wooding FBP (1982): The role of the binucleate cell in ruminant placental structure. J Reprod Fertil Suppl 31:31-39
  58. Wooding FBP (1992): Current topic: the synepitheliochorial placenta of ruminants: binucleate cell fusion and hormone production. Placenta 13:101-113 https://doi.org/10.1016/0143-4004(92)90025-O
  59. Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374 https://doi.org/10.1038/28615
  60. Wakayama T, Yanagimachi R (1999): Cloning of male mice from adult tailtip cells. Nat Genet 22:127-128 https://doi.org/10.1038/9632
  61. Young LE, Sinclair KD, Wilmut I (1998): Large offspring syndrome in cattle and sheep. Rev Reprod 3:155-163 https://doi.org/10.1530/ror.0.0030155
  62. Zou X, Chen Y, Wang Y, Luo J, Zhang Q, Zhang X, Yang Y, Ju H, Shen Y, Lao W, Xu S, DuM (2001): Production of cloned goats from enucleated ooeytes injected with cumulus cell nuclei or fused with cumulus cells. Cloning 3:31-37 https://doi.org/10.1089/152045501300189312