Reproductive and Developmental Biology

  • 제29권2호
  • /
  • Pages.63-68
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  • 2005
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Cloned Placenta of Korean Native Calves Died Suddenly at Two Months after Birth Displays Differential Protein Expression

  • Kim Hong Rye (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Kang Jae Ku (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Lee Hye Ran (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Yoon Jong Taek (Genetic Engineering Institute, Hankyong National University) ;
  • Seong Hwan Hoo (National Livestock Research Institute, RDA) ;
  • Jung Jin Kwan (National Livestock Research Institute, RDA) ;
  • Park Chang Sik (Research Center for Transgenic Cloned Pigs. Chungnam National University) ;
  • Jin Dong Il (Research Center for Transgenic Cloned Pigs. Chungnam National University)
  • 발행 : 2005.06.01
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초록

Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, $H^+-transporting$ ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.

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