The Physicochemical Properties of Seed Mash Prepared with Koji

Koji를 이용한 seed mash의 이화학적 특성

  • Lee, Myung-Koo (Department of Applied Biology and Chemistry, KonKuk University) ;
  • Park, Jung-Kil (Department of Food Engineering, Chungju University) ;
  • Lee, Si-Kyung (Department of Applied Biology and Chemistry, KonKuk University)
  • 이명구 (건국대학교 응용생물화학과) ;
  • 박정길 (충주대학교 식품공학과) ;
  • 이시경 (건국대학교 응용생물화학과)
  • Published : 2005.04.30

Abstract

Physicochemical quality characteristics of seed mashes were investigated for development of new creative breadmaking in bakery. Aridity of seed mashes fermented with Koji, lactic acid bacteria, and baker's yeast was slightly lower than those fermented with Koji and yeast. ${\alpha}-Amylase$, saccharifying amylase, and acidic protease activities of seed mash composed of water (560 mL) and Koji (400 g) were 0.26 SKB, 36 SP, and 645 HUT/g, respectively. Reducing sugar content of seed mash made with Koji increased up to 13.04% after 36 hr fermentation, then decreased drastically thereafter, whereas that of seed mashes made with Koji, yeast, and lactic acid bacteria increased up to 6.5% at 6 hr, decreased to 1 to 2.5% at 12 hr, and remained less than 0.7% after 18 hr fermentation. Total organic acid contents were 10.4-12.25mg/mL. Flavor compounds including ethyl acetate, ethyl caprylate, isoamyl acetate, and p-vinyl guaiacol were detected in seed mash fermented with Koji, yeast, and L. brevis. These results show use of seed mash fermented with Koji, S. cerevisiae, and lactic acid bacteria enhances bread flavor.

본 연구는 제빵 산업의 현장에서 제빵적성 향상을 위한 제빵법을 확립하기 위하여 백국균을 이용한 밀가루 코지, 유산균, 효모를 혼합 배양하여 seed mash를 만들어 그 특성을 분석하였다. 증류수 560mL와 코지 400g을 혼합하여 $25^{\circ}C$에서 72시간 배양한 seed mash의 효소 활성을 측정한 결과는 ${\alpha}-amylase$는 0.26 SKB/g, saccarifying amylase 활성이 36SP/g,그리고 산성 protease 활성은 648 HUT/g을 나타냈다. 코지, 유산균, 효모를 배양하여 제조한 seed mash의 pH는 코지와 효모 만을 배양하여 제조된 seed mash보다 다소 낮았다. 배양기간에 따른 환원당의 변화는 코지만을 이용한 seed mash의 경우 36시간까지 배양기간이 증가함에 따라 증가하였으며, 그 이후 감소하였다. 그러나 유산균과 효모를 첨가한 seed mash에서의 환원당은 배양 6시간까지 증가하다 그 이후 급격히 감소하였다. Seed mash에서 생성된 총산의 양은 10.4-12.25mg/mL로 유산균을 첨가시 다소 높았다. 코지, 효모, L. brevis로 배양한 seed mash에서 ethyl acetate, ethyl caprylate, isoamyl acetate, ethyl caprate, p-vinyl guaiacol 등의 향기 성분이 검출되었다.

Keywords

References

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