The Antioxidative and Antimicrobial Activities of the three Species of Leeks(Allium tuberosun R.) Ethanol Extracts

품종별 부추 추출물의 항산화성 및 항균성

  • Ahn, Myung-Soo (Department of Food and Nutrition, Sungshin Women's University) ;
  • Kim, Hyun-Jeung (Department of Food and Nutrition, Sungshin Women's University) ;
  • Seo, Mi-Sook (Department of Food and Nutrition, Sungshin Women's University)
  • 안명수 (성신여자대학교 식품영양학과) ;
  • 김현정 (성신여자대학교 식품영양학과) ;
  • 서미숙 (성신여자대학교 식품영양학과)
  • Published : 2005.04.30

Abstract

The antioxidative and antimicrobial activities were carried on the leeks (Allium tuberosun R.) ethanol extracts in order to find out new food functional components. Three species of leeks used in this study were Chinese leek(long type, LL), general leek(medium type, LM), and medicinal leek (short type, LS). Total amounts of polyphenol compounds in LS was shown as the highest (436.60mg%) value. All of ethanol extracts of these leeks were shown to be had good electron donating ability(EDA) and nitrite scavenging activity. Specialty, the ethanol extract of LS(LSEx) had the highest EDA 30.47% and nitrite scavenging activity 77.24% and the lowest was LMEx. The antioxidative activities of these ethanol extracts on the corn germ oil were measured by peroxide values(POV) and conjugated diene values (CDV) storaging for 30 days at $60{\pm}2^{\circ}C$. The antioxidative activities of these extracts by POV and CDV were determined as following order as LSEx> LLEx) LMEx. The antioxidative activities of all extract were presented as high tendency by increasing adding amounts (0.02%>0.05%>0.1%). When the antioxldative activities were compared with BHT and ${\alpha}-tocopherol$, the degree of the antioxidative activities of these extracts were certified as lower than BHT and higher than ${\alpha}-tocopherol$. And also LSEx, LMEx, and LLEx had antimicrobial effects on the several micro organisms, especially the effect on the Pseudomonas aeruginasa was remarkable. While LMEx had shown inhibit effect on most of micro organism used in this study.

본 연구결과 호부추(LL), 재래종 부추(LM) 및 약부추(LS)의 총 폴리페놀함량은 $286{\sim}437mg%$이었으며 LS> LM> LL순인 것으로 나타났다. 이들 3품종의 부추 에탄올 추출물의 전자공여능 및 아질산염 소거능도 확인되었으며 그 중에서 LSEx 가 가장 높은 수치를 보였다. 또한 유지에 대한 항산화력을 과산화물가(POV)와 공액이중결합산량으로 측정 비교한 결과 3종의 부추 에탄올추출물 모두 0.02% 첨가 종도에서 항산화 효과를 보였으며 그 정도는 ${\alpha}-tocopherol$ 보다 높고 BHT보다는 낮은 경향이었다. 이러한 효과는 첨가 농도가 높을수록 컸으며 품종 별로 보면 LSEx가 가장 높았다. 따라서 약부추와 그 에탄올추출물이 총 폴리페놀함량과 전자공여능 및 아질산 소거능과 마찬가지로 옥배유에 대한 항산화력이 가장 높은 것을 알 수 있었다. 한편 부추 3품종의 에탄올추출물 모두 항균성이 확인되었으며 특히 이들 모두 Pseudomonas에 대하여 높은 저해성을 보였으며 위의 각종 이화학적 성질이 떨어지는 것으로 나타난 재래종 부추의 에탄올추출물은 모든 균주에 고른 항균성을 나타내었다. 이러한 결과에서 3가지 품종의 부추 에탄올추출물들 중 항산화력은 소립종인 약부추가, 항균력은 중립종인 재래종 부추가 가장 높은 효과를 나타내었지만 모든 추출물들에서 항산화력과 항균력을 확인할 수 있어서 이들 3종 부추 모두 항산화와 항균성에 대한 기능성 식품으로 충분한 이용가치가 있을 것으로 사료된다.

Keywords

References

  1. 현대식물분류학 이유성;이상태
  2. 신고식물분류학 이창복
  3. J. Kor. Soc. Hort. Sci. v.44 no.6 Physiological Responses of Korean Native Chinese Chive (Allium tuberosum) Having Differents Chilling Tolerance under Chilling Treatment Kang, S.G.;Choi, Y.J.;Cho, J.G.;Chung, H.D.;Suh, S.G.
  4. Korean J of Food Preservation v.9 no.1 Effects of the Addition of Leek and Dropwort Powder on the Quality of Noodles Kim, C.B.;Lee, M.Y.;Yoon, J.T.;Cho, R.K.
  5. 월간원예 no.168 농업정보신문
  6. Korean J. Food Sci. v.7 Components and Antioxidative Activities of Buchu(Chinese chives) harvested at Different Times Moon, G.S.;Ryu, B.M.;Lee, M.J.
  7. Korean J. Food Sci. Technol. v.33 no.3 The various biofunctional effects(anticarcinogenic, antioxidative and lypolytic activity) of Pohang bchu Hwang, C.W.;Shin, H.K.;Do, M.S.;Kim, Y.J.;Park, Y.H.;Choi, Y.S.;Joo, W.H.
  8. Am. J. clin. Nutr. v.66 Efeects of garlic thioallyl derivatives on growth, glutathione concentration and polyamine formation of human prostate carcinoma cells in culture Pinto, J.T.;Qiao, C.;Xing, J.;Rivlin, R.S.;Protomastro, M.L.;Weissler, M.I.;Tao, Y.;Thaler, H.;Heston, W.D.
  9. Korean J. Soc. Food Sci v.10 no.1 Changes of antioxidative properties according to the heat-treatment of ginger extracts Lee, J.Y.;Ahn, M.S.
  10. Korean J. Sac. Food Sci v.14 Chlorophyll, mineral contents and SOD-like activities of leeks harvested at different times Kwak, Y.J.;Chun, H.J.;Kim, J.S.
  11. Korean J. Food Sci. Technol. v.33 no.3 The various biofunctional effects(anticarcinogenic, antioxidative and lypolytic activity) of Pohang bchu Hwang, C.W.;Shin, H.K.;Do, M.S.;Kim, Y.J.;Park, Y.H.;Choi, Y.S.;Joo, W.H.
  12. J. Korean Soc. Food Sci, Nutr. v.32 Effect of long term buchu(Chines chives) diet on antioxidative system of IRC mice Lee, M.J.;Ryu, B.M.;Lee, Y.S.;Moon, G.S.
  13. Ntraceut. Food v.7 Protective effect of Diet buchu(Chines chives) against oxidative damage from aging and ultraviolet irradiation in ICR mice skin Lee, M.J.;Ryu, B.M.;Kim, M.H.;Lee, Y.S.;Moon, G.S. https://doi.org/10.3746/jfn.2002.7.3.238
  14. Free Rad. Biol. Med. v.31 Influence of vitamin E and C supplementation on lipoprotein oxidation in patients with Alzheimer's disease Anatol, K.;Ulrike, M.;Sonke, A.;Amaar, U.;Charotrharlotte, L.;Tom, M.T.;Ulrike, B. https://doi.org/10.1016/S0891-5849(01)00737-7
  15. Free Rad. Biol. Med. v.31 Antioxidant mechani-c of isoflavones in lipid system: paradoxical effects of peroxyl radical scavenging Rakesh, P.P.;Brenda, J.B.;Jack, H.C.;Neil, H.;Marion, K.;Balaraman, K.;Dale, A.P.;Stephen, B.;Victor, D.U. https://doi.org/10.1016/S0891-5849(01)00737-7
  16. FEBS Lett. v.418 Comparative mechanisms and rates of free radical scavenging by carotenoid antioxidants Mortensen, A.;Skibsted, L.H.;Sampson, J.;Rice-Evans, C.;Everett, S.A. https://doi.org/10.1016/S0014-5793(97)01355-0
  17. J.Agric. Food Chem. v.47 free radical scavenging activity of grape seed extract and antioxidants by electron spin resonance spectrometry in an H202/NaOH/DMSO system Yamaguchi, F.;Yoshimura, Y.;Nakazawa, H.;Ariga, T.
  18. J. Fd hyg. Safety v.15 no.3 Separation and Identification of Antimicrobial Compounds from Korean Leek Hong, J.H.;Lee, M.H.;Kang, M.C.;Hur, S.H.
  19. Korean J. Food Sci. Technol. v.28 no.3 Antimicrobial Substances in Leek(Allium tuberosum) Kim, S.J.;Park, K.H.
  20. Official methods of analysis A.O.A.C.
  21. Korean J. Food Culture v.19 no.2 A study on the antioxidative and antimicrobial activities of the Chopi solvents extracts Ahn, M.S.;Won, J.S.;Kim, H.J.;Han, M.N.
  22. Analytical Biochemistry v.293 Oxygen Radical Absorbance Capacaity of the Phenolic Compounds in Plant Extracts Fractionated by High-Perpormance Liquid Chromatograph Caldwell, Charles R. https://doi.org/10.1006/abio.2001.5134
  23. Korean J. pharmacogn v.29 antioxidative compounds in aerial parts of potentilla fragariodes Choi, Y.H.;Kim, M.J.;Lee, H.S.;Yun, B.S.;Hu, C.;Kwak, S.S.
  24. Lebensm-Wiss-u-Technol v.28 Use of free radical method to evaluate antioxidant Williams, B.W.;Cuvelier, M.E.;Berset, C. https://doi.org/10.1016/S0023-6438(95)80008-5
  25. Agric. Biol. Chem. v.51 Inhibition of nitrosamine formation by nondialyzable melanoidins Kato, H.;Lee, I.E.;Chuyen, N.V.;Kim, S.B.;Hayase, F. https://doi.org/10.1271/bbb1961.51.1333
  26. Official and tentative methods A.O.C.S.
  27. Effects of reaction temperature, time and persence of orgarnic acids or their salts on the antioxidants activity of caramelization mixture Ahn, M.S.
  28. J. Living Culture Research v.15 A study on the antioxidative and antimicrobial activities of the Applenmint solvents extracts. Sungshin women's university Ahn, M.S.;Kim, H.J.
  29. Food Techonol v.1 Methods for testing the efficacy of food antimicrobials Davison, P.M.;Parish, M.E.
  30. Food Technol v.40 Antimicrobial agents Judie, D.D.
  31. Korean J. Food Preser. v.9 Antioxidative and free radical scavenging activity of water extract from dandelion Kang, M.J.;Shin, S.R.;Kim, K.S.