한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제13권1호
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  • Pages.48-51
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  • 2005
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

깽깽이풀의 핵형분석과 McFISH를 이용한 rDNA의 물리지도 작성

Karyotype Analysis and Physical Mapping of rDNAs Using McFISH in Jeffersonia dubia Benth

  • 김수영 (충남대학교 생명과학부) ;
  • 최혜운 (충남대학교 생명과학부) ;
  • 구달회 (충남대학교 생명과학부) ;
  • 김찬수 (산림과학원 난대산림연구소) ;
  • 방재욱 (충남대학교 생명과학부)
  • Kim, Soo-Young (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Choi, Hae-Woon (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Koo, Dal-Hoe (School of Bioscience & Biotechnology, Chungnam Natl. Univ.) ;
  • Kim, Chan-Soo (Warm-Temperate Forest Research Center, Korea Forest Research Institute) ;
  • Bang, Jae-Wook (School of Bioscience & Biotechnology, Chungnam Natl. Univ.)
  • 발행 : 2005.02.28
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초록

보호식물이며, 약용식물인 깽깽이풀 (Jeffersonia dubia)을 대상으로 핵형 분석과 McFISH 기법을 이용한 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 체세포 염색체 수는 2n=2x=12였으며, 2쌍의 중부 염색체 (염색체 1, 3), 2쌍의 차중부 염색체 (염색체 2, 4) 그리고 2쌍의 차단부 염색체 (염색체 5, 6)로 구분되었고, 염색체의 평균 길이는 $1.95{\sim}3.50{\mu}M$이었다. McFISH기법을 이용하여 45S와 5S rDNA의 염색체상의 위치를 확인한 바, 3쌍의 45S rDNA signal은 4번, 5번 그리고 6번 염색체의 단완 말단 부위에서 관찰되었고, 한 쌍의 5S rDNA signal은 2번 염색체의 동원체 부위에서 관찰되었다.

Karyotype analysis and chromosomal locailization of 45S and 5S rDNAs using McFISH (multi-color fluorescence in situ hybridization) were carried out in Jeffersonia dubia Benth., which is one of medicinal plants belonging to Berberidaceae. The somatic metaphase chromosome number was 2n=2x=12 and the size of chromosomes ranged $1.95{\sim}3.50\;{\mu}m$. The chromosome complement consisted of two pairs of metacentrics (chromosomes 1 and 3), two pairs of submetacentrics (chromosomes 2 and 4) and two pairs of subtelocentrics (chromosomes 5 and 6). In McFISH, one pair of 45S rDNA site was detected on the centromeric region of chromosome 2 and three pairs of 5S rDNA sites were detected on the short arm of chromosomes 4, 5 and 6, respectively.

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참고문헌

  1. The Medicinal Plants of Korea Bae, K.H.
  2. Caryologia v.8 Chromosome morphology and terminology Battalia, E. https://doi.org/10.1080/00087114.1955.10797556
  3. Korean J. Medicinal Crop Sci. v.12 Physical Mapping of rDNAs using McFISH in Anemarrhena asphodeloides Bunge Kim, S.Y.;Choi, H.W.;Bang, J.W.
  4. Korean J. Genetics v.20 Cytogenetic analysis of Brassica campestris var. pekinensis using C-banding and FISH Kim, S.Y.;Lim, Y.P.;Bang, J.W.
  5. Korean J. Medicinal Crop Sci. v.10 Karyotype analysis of Anemarrhena asphodeloides Bunge Kim, S.Y.;Koo, D.H.;Bang, J.W.
  6. Korean J. Medicinal Crop Sci. v.12 Cytogenetic analysis of four Hosta species native to Korea Kim, H.H.;Park, Y.W.;Yoon, P.S.;Choi, H.W.;Bang, J.W.
  7. Korean J. Medicinal Crop Sci. v.12 Karyotype analysis of eight Korean native species in the genus Iris Kim, H.H.;Park, Y.W.;Yoon, P.S.;Choi, H.W.;Bang, J.W.
  8. Korean J. Medicinal Crop Sci. v.5 Karyotype analysis and physical mapping of rDNAs in Bupleurum longeradiatum Koo, D.H.;Seong, N.S.;Soong, J.S.;Bang, K.H.;Bang, J.W.
  9. Korean J. Plant Biotech. v.30 Cytogenetic analyses of Angelica plants using Feulgen staining and multicolor fluorescence in situ hybridization Koo, D.H.;Kim, S.Y.;Bang, K.H.;Seong, N.S.;Bang, J.W. https://doi.org/10.5010/JPB.2003.30.2.123
  10. FISH mapping of rDNAs and repeated DNA sequences on pachytene chromosomes of cucumber (Cucumis sativus L.). Plant & Animal genome XI Koo, D.H.;Hur, Y.K.;Bang, J.W.
  11. Mem. Ehime Univ. Sect. II (Science), Ser. B Karyotype studies in Berberidaceae. 1 Kurita, M.
  12. Svensk Bot. Tidskrift v.22 Einige beobachtungen uber die zytologie der Berberidaceen Langlet, O.
  13. Illustrated Flora of Korea Lee, C.B.
  14. Korean J. Medicinal Crop Sci. v.12 Karyotype analysis of five species of genus Pulsatilla Lee, W.K.;Choi, H.W.;Bang, J.W.
  15. lnst. Better Living v.8 Chromosome number of flowering plants in Korea (4) Korean Res. Lee, Y.N.
  16. Genome v.36 Physical mapping of four sites of rDNA sequences and one site of the ${\alpha}$-amylase-2 gene in barley (Hordeum vulgare) Leitch, I.J.;Heslop-Harrison, J.S. https://doi.org/10.1139/g93-071
  17. Hereditas v.52 Nomenclature for centromeric position in chromosomes Levan, A.;Frekga, K.;Sandberg, A. https://doi.org/10.1111/j.1601-5223.1964.tb01953.x
  18. Plant J. v.1 Localization of tandemly repeated DNA sequences in Arabidopsis thaliana Maluszynska, J.;Heslop-Harrison, J.S. https://doi.org/10.1111/j.1365-313X.1991.00159.x
  19. Genome v.36 Simultaneous discrimination of the three in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes Mukai, Y.;Nakahara, Y. https://doi.org/10.1139/g93-067
  20. Plant J. v.12 Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones Murata, M.;Heslop-Harrison, J.S.;Motoyoshi, F. https://doi.org/10.1046/j.1365-313X.1997.12010031.x
  21. Genetica v.62 A practical method of chromosome classification on the basis of centromere position Naranjo, C.A.;Poggio, L.;Brandham, P.E. https://doi.org/10.1007/BF00123310
  22. Theor. Appl. Genet. v.91 Cytological studies of African cultivated rice, Oryza glaberrima Ohmido, N.;Fukui, K.
  23. Applied Entomology & Zoology v.32 Larvicidal and antifeeding activities of Oriental medicinal plant extracts against four species od forest insect pests Park, S.J.;Lee, S.G.;Shin, S.C.;Lee, B.Y.;Ahn, Y.J. https://doi.org/10.1303/aez.32.601