생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

토복령의 항산화 활성

Antioxidant Activity of Smilacis Chinae Radix

  • 이은희 (상지대학교 생명자원과학대학 바이오산업공학과) ;
  • 노미애 (상지대학교 생명자원과학대학 바이오산업공학과) ;
  • 차배천 (상지대학교 생명자원과학대학 바이오산업공학과)
  • Lee, Eun-Hee (Department of Bio-Industry and Technology, Sangji University) ;
  • Noh, Mi-Ae (Department of Bio-Industry and Technology, Sangji University) ;
  • Cha, Bae-Cheon (Department of Bio-Industry and Technology, Sangji University)
  • 발행 : 2005.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Smilacis Chinae Radix is root of Smilax china L.. It has been known as a korean folk medicine for anti-inflammatory, anti-microbial diuresis, detoxification and relieving enteropathy as diarrhea. Reactive oxygen species(ROS) are continuously produced at a high rate as a by-product obtained in the aerobic metabolism. A major portion of living organisms has defense system as superoxide dismutase or catalase against damage produced by ROS. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. In this study, we have investigated the antioxidant activities of Smilacis Chinae Radix in order to screen the antioxidant substances from natural products. As a result, EtOAc extract of Smilacis Chinae Radix exhibited potent antioxidant effect on various antioxidant experiment. The major components of antioxidant activity were isolated from EtOAc extract of Smilacis Chinae Radix. Their structure of compounds were identified as quercetin and (-)-epicatechin by spectroscopic evidence, respectively.

키워드

참고문헌

  1. Kim, T. J. (1993) Alpine flowers of korea, 542. Kyo Hak Sa, Seoul
  2. Park, J. H., Kim, J. M. and Do, W. I. (2002) Pharmacognostical study on the To Bog Ryung. Kor. J. Pharmacogn. 33: 169-172
  3. Son, K. H., Seo, J. H., Lee, J. M., Kwon, S. J., Chang, S. Y. and Lee, K. S. (2001) Isolation and quantitative determination of dioscin from Smilacis Chinae Radix. Kor. J Pharmacogn. 32: 153-156
  4. Song, J. H., Kwon, H. D., Lee, W. K. and Park, I. H. (1997) Antimicrobial activity of crude extracts frorn Smilax China Root. Kor. J. Biotechnol. Bioeng. 12: 560-564
  5. Kim, S. W., Chung, K. C., Son, K. H. and Kang, S. S. (1989) Steroidal saponins from the Rhizomes of Smilax china. Kor. J. Pharmacogn. 20: 76-82
  6. Lee, S. O., Kim, M. J., Kim, D. K. and Choi, H. J. (2005) Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. Korean J. Soc. Food Sci. Nutr. 34: 139cI47
  7. Papa, S. and Skulachev, V P. (1997) Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell Biochem. 174: 305-319 https://doi.org/10.1023/A:1006873518427
  8. Fukuzawa, K. and Takaishi, Y. (1990) Antioxidants. J. Act. Oxyg. Free Rad. 1: 55-70
  9. Halliwell, B. (1991) Drug antioxidant effects. Drugs, 42: 569-605 https://doi.org/10.2165/00003495-199142040-00003
  10. Cerutti, P. A. (1994) Oxy-radicals and cancer. Lancet. 344: 862-863 https://doi.org/10.1016/S0140-6736(94)92832-0
  11. Halliwell, B. and Gutteridge, J. M. (1984) Lipid peroxidation, oxygen radical, cell damage and anti-oxidant therapy. Lancet. 23: 1396-1397
  12. Corl, M. M. (1974) Antioxidant activity of tocopherol and ascorbylpalmitate and their mode of action. JAOCS. 51: 321-325 https://doi.org/10.1007/BF02633006
  13. Coleman, M. D., Fernandes, S. and Khanderia, L. A. (2003) A preliminary evaluation of a novel method to monitor a triple antioxidant combination (vitamin E, C and $\alpha$-lipoic acid) in diabetic volunteers using in vitro methaemoglobin formation. Environ. Toxicol. Pharmacol. 14: 69-75 https://doi.org/10.1016/S1382-6689(03)00027-9
  14. Monsen, E. R. (2000) Dietary reference intakes for the antioxidant nutrients : vitamin C, vitamin E, selenium, and carotenoids, J. Am. Diet. Assoc. 100: 637-640 https://doi.org/10.1016/S0002-8223(00)00189-9
  15. Ito, N., Fukushima, S., Hagiwara, A., Shibata, M. and Ogiso, T. (1983) Carcinogenicity of butylated hydroxyanisole on F 344 rats. J. Nail. Cancer Inst. 70: 343-352
  16. Branen, A. L. (1975) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. JAOCS. 52: 59-63 https://doi.org/10.1007/BF02901825
  17. Yu, w., Zhao, Y and Shu, B. (2004) The radical scavenging activities of radix puerariae isoflavonoids. Food Chem. 86: 525-529 https://doi.org/10.1016/j.foodchem.2003.09.005
  18. Choi, S. I., Lee, Y. M. and Heo, T. R. (2003) Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of traditional herbal medicine extracts. Korean. J. Biotechnol. Bioeng. 18: 282-288
  19. Fukumoto, L. R. and Mazza, G (2000) Assessing antioxidant and peroxidant activities of phenolic compounds. J. Agric. Food Chem. 48: 3597-3604 https://doi.org/10.1021/jf000220w
  20. Lim, D. K., Choi, U. and Shin, D. H. (1996) Antioxidative activity of ethanol extract from Korean medicinal plants Korean. J. Food Sci. Technol. 28: 83-89
  21. Uchiyama, M., Suzuki, Y. and Fukuzawa, K. (1968) Biochemical studies of physiological function of tocopheronolactone. 1. Yakugaku Zasshi, 88: 678-683
  22. Yoshikawa, M., Harada, E., Miki, A., Tsukamoto, K., Liang, S. Q., Yamahara, J. and Murakami, N. (1994) Antioxidant constituents from the fruit hulls of Mangosteen (Garcinia mangos/ana L.) originating in vietnam. Yakugaku Zasshi, 114: 129-133
  23. Inatani, R., Nakatani, N. and Fuwa, H. (1983) Antioxidative effect of the constituents of rosemary (Rosemaries officinalis L.) and their derivatives. Agric. Biol. Chem. 47: 521-528 https://doi.org/10.1271/bbb1961.47.521
  24. Chen, C. Wand Ho, C. T. (1995) Antioxidant properties of polyphenols extracted from green and black teas. J. Food Lipids, 2: 35-46 https://doi.org/10.1111/j.1745-4522.1995.tb00028.x
  25. Lim, D. K., Choi, U. and Shin, D. H. (1996) Antioxidative of some solvent extract from Caesalpinia sappan L. Korean J. Food Sci. Technol. 28: 77-82
  26. Choe, S. Y. and Yang, K. H. (1982) Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BRA). Korean J. Food Sci. Technol. 14: 283-288
  27. Yoon, I., Wee, J. H., Moon, J. H., Ahn, T. H. and Park, K. H. (2003) Isolation and identification of quercetin with antioxidative activity from the fruits of Rubus coreanum Miquel. Korean J. Food Sci. Technoi. 35: 499-502
  28. Peng, Z. F., Strack, D., Baumert, A., Subramaniam, R., Goh, N. K, Chia, T. F., Tan, S. N. and Chia, L. S. (2003) Antioxidant flavonoids from leaves of Polygonum hydropiper L. Phytochemistry, 62: 219-228 https://doi.org/10.1016/S0031-9422(02)00504-6
  29. Witting, J., Herderich, M., Graefe, E. U. and Veit, M. (2001) Identification of quercetin glucuronides in human plasma by high-performance liquid chromatography-tandem mass spectrometry. J. Chromatography B. 753: 237-243 https://doi.org/10.1016/S0378-4347(00)00549-1
  30. Natsume, M., Osakabe, N., Oyama,M., Sasaki, M., Baba, S., Nakamura, Y., Osawa, T. and Terao, J. (2003) Structure of (- )-epicatechin glucuronide identified from plasma and urine after oral ingestion of (- )-epicatechin : differences between human and rat. Free Radical Biol. Med. 34: 840-849 https://doi.org/10.1016/S0891-5849(02)01434-X
  31. Berregi, I., Santos, J. I., Campo, G and Miranda, J. I. (2003) Quantitative determination of (-)-epicatechin in cider apple juices by $^1$H-NMR. Talamo, 61: 139-145
  32. Cren-Olive, C., Wieruszeski, J. M., Maes, E. and Rolando, C. (2002) Catechin and epicatechin deprotonation followed by $^{13}$C_NMR. Tetrahedron Lett. 43: 4545-4549 https://doi.org/10.1016/S0040-4039(02)00745-1