Journal of Acupuncture Research
- Volume 22 Issue 6
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- Pages.1-15
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
The Effects of Peucedani Radix Herbal Acupuncture at St36 on Ovalbumin-induced Asthma in C57BL Mouse
전호약침(前胡藥鍼)이 Ovalbumin-induced Asthma Mouse Model에 미치는 영향
- Roh, Hong-Pyo (Department of Cardiovascular & Neurologic Disease, College of Oriental Medicine, Dae-Jeon University) ;
- Seol, In-Chan (Department of Cardiovascular & Neurologic Disease, College of Oriental Medicine, Dae-Jeon University) ;
- Kim, Yoon-Sik (Department of Cardiovascular & Neurologic Disease, College of Oriental Medicine, Dae-Jeon University)
- Published : 2005.12.20
Abstract
Objectives : The aim of this study was to investigate the effect of Peucedani Radix herbal acupuncture(PR-HA) at St36(joksamni) on ovalbumin-induced asthma in mice. Methods : C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week) C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (3times a week). One of the two experimental groups was just treated with needle-prick on St36 and the other group was treated with 1% concentrations of PR-HAS at St36, for the later 8 weeks (3times /week). Results : 1. The weight and total cells of lung of the mice group treated with PR-HA decreased significantly compared with those of control group. 2. Total Leukocytes and Eosinophils in BALF of the mice group treated with PR-HA decreased significantly compared with those of control group. 3. Eosinophils in BALF of the mice group treated with PR-HA in Photomicrographs decreased significantly compared with those of control group. 4. According to Histological analysis of lung sections, it decreased significantly adhension of collagen in PR-HA than those of control group 5. The concentration of IgE, IL-4, IL-5, in BALF and IL-4, IL-5, Il-13 in serum of the mice group treated with PR-HA decreased significantly compared with that of control group. 6. The number of Gr-1+/CD11b+, CD11b+, CD3-/CCR3+, CD4+, CD3e+/CD69+ , CD23+B220+ cells in the lungs of the mice group treated with PR-HA decreased significantly compared with those of control group. 7. The cytokine's manifestation of mRNA of the mice group treated with PR-HA with RT-PCR decreased significantly compared with that of control group. Conclusion : We conclude that PR-HA is effective on OVA-induced asthma of C57BL/6 mouse.