Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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The Schizosaccharomyces pombe Gene Encoding ${gamma}-Glutamyl$ Transpeptidase I Is Regulated by Non-fermentable Carbon Sources and Nitrogen Starvation

  • Kim, Hong-Gyun (Division of Life Sciences, Kangwon National University) ;
  • Park, Hey-Jung (Division of Life Sciences, Kangwon National University) ;
  • Kang, Hyun-Jung (Division of Life Sciences, Kangwon National University) ;
  • Lim, Hye-Won (Division of Life Sciences, Kangwon National University) ;
  • Kim, Kyung-Hoon (Division of Life Sciences, Kangwon National University) ;
  • Park, Eun-Hee (College of Pharmacy, Sookmyung Women's University) ;
  • Ahn, Ki-Sup (Department of Health and Environment, Baekseok College) ;
  • Lim, Chang-Jin (Division of Life Sciences, Kangwon National University)
  • Published : 2005.02.28
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Abstract

In our previous study, the first structural gene (GGTI) encoding ${\gamma}-glutamyl$ transpeptidase was cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its transcription, using the GGTI-lacZ fusion gene, containing the 1,085 bp upstream region from the translational initiation point, was found to be enhanced by sodium nitroprusside and L-buthionine-(S,R)-sulfoximine (BSO). In the present work, regulation of the GGTI gene was further elucidated. Non-fermentable carbon sources, such as acetate and ethanol, markedly enhanced the synthesis of ${beta}-galactosidase$ from the GGTI-lacZ fusion gene. However, its induction by non-fermentable carbon sources appeared to be independent of the presence of the Pap1 protein. Nitrogen starvation also gave rise to induction of GGTI gene expression in a Pap1-independent manner. The three additional fusion plasmids, carrying 754, 421 and 156 bp regions, were constructed. The sequence responsible for the induction by non-fermentable carbon sources and nitrogen starvation was identified to exist within a -421 bp region of the GGTI gene. Taken together, the S. pombe GGTI gene is regulated by non-fermentable carbon sources and nitrogen starvation.

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References

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