Macromolecular Research

The Polymer Society of Korea (한국고분자학회)
권호 표지

Chondrogenic Differentiation of Bone Marrow Stromal Cells in Transforming Growth $Factor-{\beta}_{1}$ Loaded Alginate Bead

  • Park, Ki-Suk (Department of Polymer Nano Science and Technology, Chonbuk National University) ;
  • Jin Chae-Moon (Department of Polymer Nano Science and Technology, Chonbuk National University) ;
  • Kim, Soon-Hee (Department of Polymer Nano Science and Technology, Chonbuk National University) ;
  • Rhee John M. (Department of Polymer Nano Science and Technology, Chonbuk National University) ;
  • Khang Gil-Son (Department of Polymer Nano Science and Technology, Chonbuk National University) ;
  • Han, Chang-Whan (Department of Orthopedic Surgery, College of Medicine, The Catholic University) ;
  • Yang, Yoon-Sun (Medipost Co., West Bldg., 3rd floor, Doosan Technical Institute) ;
  • Kim, Moon-Suk (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology) ;
  • Lee, Hai-Bang (Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology)
  • Published : 2005.08.31
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Abstract

We developed alginate beads loaded with transforming growth $factor-{\beta}_{1}(TGF-{\beta}_{1})$ to examine the possible application of the scaffold and cytokine carrier in tissue engineering. In this study, bone marrow stromal cells (BMSCs) and $TGF{\beta}_{1}$ were uniformly encapsulated in the alginate beads and then cultured in vitro. The cell morphology and shape of the alginate beads were observed using inverted microscope, scanning electron microscope (SEM), histological staining and RT-PCR to confirm chondrogenic differentiation. The amount of the $TGF{\beta}_{1}$ released from the $TGF-{\beta}_{1}$ loaded alginate beads was analyzed for 28 days in vitro in a phosphate buffered saline (pH 7.4) at $37^{\circ}C$. We observed the release profile of $TGF-{\beta}_{1}$ from $TGF-{\beta}_{1}$ loaded alginate beads with a sustained release pattern for 35 days. Microscopic observation showed the open cell pore structure and abundant cells with a round morphology in the alginate beads. In addition, histology and RT-PCR results revealed the evidence of chondrogenic differentiation in the beads. In conclusion, these results confirmed that $TGF-{\beta}_{1}$ loaded alginate beads provide excellent conditions for chondrogenic differentiation.

Keywords

References

  1. G. Khang, S. J. Lee, M. S. Kim, and H. B. Lee, in Webster's Biomedical Engineering Handbook, S. Webster, Ed., John & Wiley Press, NY, in press, 2005
  2. G. Khang, J. H. Lee, and H. B. Lee, in CRC Biomedical Engineering Handbook, 2nd Ed., J. Bronzio, Ed., CRC Press, Boca Raton, FL, 2000, Section IV, Chap. 39, pp 1-23
  3. G. Khang and H. B. Lee, in Methods of Tissue Engineering, A. Atala and R. Lanza, Eds., Academic Press, 2001, Section II, Chap. 20, pp 771-780
  4. G. Khang and H. B. Lee, Biomedical Polymers, Korean Chemical Society Press, Munundang, Seoul, Korea, 2001
  5. E. K. Jeon, H. J. Whang, G. Khang, I. Lee, J. M. Rhee, and H. B. Lee, Polymer(Korea), 25, 893 (2001)
  6. E. K. Jeon, J. Y. Shim, H. J. Whang, G. Khang, I. Jo, I. Lee, J. M. Rhee, and H. B. Lee, Biomater. Res., 5, 23 (2001)
  7. J. W. Jang, B. Lee, C. W. Han, I. Lee, H. B. Lee, and G. Khang, Polymer(Korea), 27, 226 (2003)
  8. G. Khang, E. K. Jeon, J. M. Rhee, I. Lee, S. J. Lee, and H. B. Lee, Macromol. Res., 11, 334 (2003) https://doi.org/10.1007/BF03218373
  9. H. B. Lee, G. Khang, and S. J. Lee, in Tissue Engineering and Regenerative Medicine, J. J. Yoo and I. Lee, Eds., Gunja Pub., Seoul, 2002, Chap. 41, pp 751-770
  10. G. Khang, S. J. Lee, J. M. Rhee, and H. B. Lee, in Tissue Engineering and Regenerative Medicine, J. J. Yoo and I. Lee, Eds., Gunja Pub., Seoul, 2002, Chap. 17, pp 297-322
  11. S. J. Lee, I. Lee, Y. M. Lee, H. B. Lee, and G. Khang, J. Biomater. Sci. Polym. Ed., 15, 1003 (2004) https://doi.org/10.1163/1568562041526487
  12. J. W. Jang, B. Lee, C. W. Han, M. S. Kim, S. H. Cho, H. B. Lee, and G. Khang, Polymer(Korea), 28, 382 (2004)
  13. H. J. Hwang, G. Khang, J. H. Sung, I. Lee, and H. B. Lee, Biomater. Res., 6, 45 (2002)
  14. G. Khang, P. K. Shin, I. Y. Kim, B. Lee, S. J. Lee, Y. M. Lee, H. B. Lee, and I. Lee, Macromol. Res., 10, 158 (2002) https://doi.org/10.1007/BF03218266
  15. M. F. Pittenger, A. M. Mackay, S. C. Beck, and S. Craig, Science, 284, 143 (1999) https://doi.org/10.1126/science.284.5411.143
  16. S. P. Baldwin and W. M. Saltzman, Adv. Drug Del. Rev., 33, 71 (1998) https://doi.org/10.1016/S0169-409X(98)00021-0
  17. Y. Tabata, PSTT, 3, 80 (2000)
  18. R. Langer, J. Control. Rel., 62, 7 (1999) https://doi.org/10.1016/S0168-3659(99)00057-7
  19. T. A. Holland and A. G. Mikos, J. Control. Rel., 86, 1 (2003) https://doi.org/10.1016/S0168-3659(02)00373-5
  20. S. Kondo, S. H. Cha, W. F. Xie, and L. J. Sandell, J. Ortho. Res., 19, 712 (2001) https://doi.org/10.1016/S0736-0266(00)00068-1
  21. H. Mizuta, A. Sanyal, T. Fukumoto, J. S. Fitzsimmons, N. Matsui, M. E. Bolander, M. J. Oursler, and S. W. O'Driscoll, J. Ortho. Res., 20, 565 (2002)
  22. J. W. Lee, W. N. Qi, and S. P. Scully, J. Ortho. Res., 20, 66 (2002) https://doi.org/10.1016/S0736-0266(01)00073-0
  23. A. Vila, A. Sánchez, M. Tobío, P. Calvo, and M. J. Alonso, J. Control. Rel., 78, 15 (2002) https://doi.org/10.1016/S0168-3659(01)00486-2
  24. W. R. Gombotz and S. F. Wee, Adv. Drug Del. Rev., 31, 267 (1998) https://doi.org/10.1016/S0169-409X(97)00124-5
  25. R. Huang, Y. Du, and J. Yang, Carbo. Polymer, 52, 19 (2003)
  26. J. H. Kim, J. M. Oh, G. Khang, J. K. Jeong, J. S. Lee, S. Y. Jeung, and H. B. Lee, J. Kor. Pharm. Sci., 32, 277 (2002)
  27. M. Weber, A. Steinert, A. Jork, A. Dimmler, F. Thurmer, N. Schutze, C. Hendrich, and U. Zimmermann, Biomaterials, 23, 2003 (2002) https://doi.org/10.1016/S0142-9612(01)00329-5
  28. K. S. Park, E. J. Kim, M. S. Kim, S. H. Cho, J. M. Rhee, H. B. Lee, and G. Khang, Biomater. Res., 8, 14 (2004)
  29. M. Mierisch, B. Cohen, C. Jordan, and G. Robertson, J. Arthro. Rel., 18, 892 (2002) https://doi.org/10.1016/S0749-8063(02)70093-5
  30. R. J. Mumper, A. S. Huffman, P. A. Puolakkainen, L. S. Bouchard, and W. R. Gombotz, J. Control. Rel., 30, 241 (1994) https://doi.org/10.1016/0168-3659(94)90030-2
  31. G. W. Vandenberg, C. Drolet, S. L. Scott, and J. de la Noue, J. Control. Rel., 77, 297 (2001) https://doi.org/10.1016/S0168-3659(01)00444-8
  32. K. Fu, A. M. Klibanov, and R. Langer, Nature Biotech., 18, 24 (2000) https://doi.org/10.1038/71875
  33. A. Kikuchi, M. Kawabuchi, M. Sugihara, Y. Sakurai, and T. Okano, J. Control. Rel., 47, 21 (1997) https://doi.org/10.1016/S0168-3659(96)01612-4