Preliminary X-ray Studies of a New Crystal form of 28 kDa Clonorchis sinensis Glutathione S-Transferase

  • Cho, Youn-Hye (School of Life Sciences, Chungbuk National University) ;
  • Kim, Young-Kwan (School of Life Sciences, Chungbuk National University) ;
  • Kim, Seung-Joon (Center for Cellular Switch Protein Structure and Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Hong, Seong-Jong (Department of Parasitology, College of Medicine, Chung-Ang University) ;
  • Chung, Yong-Je (School of Life Sciences, Chungbuk National University)
  • Published : 2005.12.31

Abstract

A new crystal of helminth glutathione S-transferase, 28 kDa isozyme from Clonorchis sinensis has been grown from a 20% PEG MME 550 solution containing 50 mM $CaCl_{2}$ in 0.1 M bis-Tris buffer (pH 6.5) in $2{\sim}3$ days. The crystals diffract to $3.0{\AA}$ resolution and belong to the orthorhombic space group $P2_{1}2_{1}2_{1}$ with cell parameters $a=62.58{\AA},\;b=69.92{\AA},\;and\;c=339.67{\AA}$.

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References

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