Discovery of Performance Traits-Linked Microsatellite Markers in Channel Catfish (Ictalurus punctatus)

  • 발행 : 2005.05.25

초록

Genomics research has two ultimate applied goals: to Isolate and clone genes of economic importance for bio-technology and gene-assisted selection (GAS), and to locate and use markers for marker-assisted selection (MAS) in selective breeding programs. To this end, we have identified linked markers for feed conversion efficiency growth rate, and disease resistance to enteric septicemia of catfish (ESC). Three microsatellite markers Ip266, Ip384, and Ip607 were identified to be linked to feed conversion efficiency. Similarly one marker each was identified to be linked to growth rate (Ip607) and disease resistance to ESC (Ip477). Ip607 marker linked to both growth rate and feed conversion efficiency, indicating that the QTL for both growth rate and feed conversion efficiency may either be the same or located in the same chromosomal region in the catfish genome. On phenotypic evaluation, certain traits such as growth rate can be accurately evaluated by body weight evaluation while other traits such as disease resistance can be quite complex. The linked DNA markers will be highly useful for MAS programs and for directing further efforts of genomic mapping for important quantitative traits.

키워드

참고문헌

  1. Hughes, C. R. and D. C. Queller, 1993. Detection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism. Mol. Ecol., 2, 131-137 https://doi.org/10.1111/j.1365-294X.1993.tb00102.x
  2. Liu, Z. J., P. Li, B. J. Argue and R. A. Dunham, 1998a. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I.furcatus) and their F1, F2, and backcross hybrids. Anim. Genet., 29, 58-62 https://doi.org/10.1046/j.1365-2052.1998.00284.x
  3. Liu, Z., A. Nichols, P. Li and R. A. Dunham, 1998b.Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus ,punctatus), blue catfish (I jUrcatus), and their F1, F2, and backcross hybrids. Mol. Gen. Genet., 258, 260-268
  4. UU, Z. J., Tan G, H. Kucuktas, P. Li, A. Karsi, D. R. Yant and R. A. Dunham, 1999. High levels of conservation at microsatellite loci among ictalurid catfishes. J. Hered., 90, 307-312 https://doi.org/10.1093/jhered/90.2.307
  5. Ozaki, A., T. Sakamoto, S. Khoo, K. Nakamura, M. R. Coimbra, T. Akutsu and N. Okamoto, 2001. Quantitative trait loci (QTLs) associated with resistance/susceptibility to infectious pancreatic necrosis virus (lPNV) in rainbow trout (Oncorhynchus mykiss). Mol. Genet. Genomics, 265, 23-31 https://doi.org/10.1007/s004380000392
  6. Tan, G, A. Karsi, P. Li, S. Kim, X. Zheng, H. Kucuktas, B. J. Argue, R. A. Dunham and Z. J. Liu, 1999. Polymorphic microsatellite markers in Ictalurus punctatus and related catfish species. Mol. Ecol., 8, 1758-1760 https://doi.org/10.1046/j.1365-294x.1999.00723-3.x
  7. Young, W. P., P. A. Wheeler, V. H. Coryell, P. Keirn and G H. Thorgaard, 1998. A detailed linkage map of rainbow trout produced using doubled haploids. Genetics, 148, 839-850