Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Hovenia dulcis Thunb Extract on the Hyperglycemic Mice Induced with Streptozotocin

자구자(Hovenia dulcis Thunb) 추출물이 Streptozotocin으로 유발된 고혈당 생쥐에 미치는 영향

  • Published : 2005.06.01
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Abstract

This study has been carried out to understand the effect of Hovenia dulcis on the hyperglycemic mice induced with streptozotocin (STZ). Mice in control group were administered with $0.9\%$ saline (2 mL/kg), and experimental groups were administered Hovenia dulcis extract (H1 group, 0.01 g/kg: H2 group, 0.04 g/kg) after hyperglycemic state was induced. Blood glucose concentrations of tile H1 and H2 groups administered with Hovenia dulcis extract for 6 weeks were significantly (p<0.01), compared to control group. Blood glucose tolerance was more favorable in H1 and H2 groups than control group. The Langerhan's islet of pancreas was destructed by treatment of STZ in the control group, but pancreatic islet of the experimental groups was partially recovered from damage, and a number of insulin-positive cells were observed. A number of insulin-like growth factor- I and II (IGF-I and IGF-II) positive cells occurred in the acinar cells of H1 and H2 groups. These results suggest that administration of Hovenia dulcis extract help mice recover from the damage induced with STZ.

본 연구는 streptozotocin(STZ)을 투여하여 고혈당이 유발된 생쥐에 지구자(Hovenia dulcis) 추출물의 투여가 미치는 영향을 구명하기 위하여 수행하였다. 대조군(Con군)은 STZ로 고혈당을 유발한 후 생리식염수 2 mL/kg를 투여하였으며, 실험군은 고혈당이 유발된 생쥐에 지구자 추출물 0.01 g/kg(H1군)과 0.049/kg(H2군)를 매일 5주간 구강 투여하였다. 체중은 CG군이나 H1군에 비하여 H2군에서 가장 높았다. 혈당량은 대조군에 비하여 H1군과 H2군 모두에서 전반적으로 유의성있게 감소하였다. 당내성 검사 결과 대조군에 비하여 H1군과 H2군 모두에서 우수한 소견을 보여주었다. 인슐린 면역조직화학 결과 대조군 췌장의 췌장섬은 파괴되어 있었으나, H1군과 H2군에서는 인슐린-양성 세포들이 다수 관찰되었다. IGF-I과 II의 면역조직화학 결과 대조군에서는 소수의 샘포세포에서 양성반응을 보였으나, H2군에서는 다수의 샘포세포에서 아주 높은 면역반응성을 보여 주었다. 이상의 결과로 보아 지구자 추출물은 STZ로 유발된 손상으로부터 $\beta$-세포의 회복 또는 재생과 췌장 샘포세포의 IGF발현에 관여하는 것으로 사료되었다.

Keywords

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