Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Anticancer Activities of Ginseng Treated with Traditional Rice Wine Steam Process Method

전통적 탁주증자법으로 처리한 홍삼의 일부 항산화 및 항암효과

  • Ye, Eun-Ju (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Kim, Soo-Jung (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Park, Chang-Ho (Dept. of Oriental Medicine Biofood Science, Daegu Haany University) ;
  • Bae, Man-Jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University)
  • 예은주 (대구한의대학교 한방바이오 식품과학과) ;
  • 김수정 (대구한의대학교 한방바이오 식품과학과) ;
  • 박창호 (대구한의대학교 한방바이오 식품과학과) ;
  • 배만종 (대구한의대학교 한방바이오 식품과학과)
  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to develop the new processing method for ginseng. To investigate the efficacy of the new product (the traditional rice wine steamed-red ginseng: RWS-RGS), antioxidant and anticancer effects of RWS-RGS were examined. The DPPH radical scavenging effect of RWS-RGS extracted with ethanol was increased in dose-dependent manner Especially, A3 ($3^{rd}$ traditional rice wine steamed-red ginsengs) exhibited effective DPPH radical scavenging activity. Nitrite scavenging effect of white ginseng (W.G), red ginseng (R.G) and RWS-RGS ($A1\~A9:\;1^{st}$ traditional rice wine steamed-red $ginseng\~9^{th}$ traditional rice wine steamed-red ginseng) were $25.9{\pm}4.4\%,\;12.9{\pm}1.1\%\;and\;26.2{\pm}0.1\~56.1{\pm}0.6\%$ at pH 1.2, respectively. The antitumor effects of W.G, R.G and RWS-RGS (A9) were examined in Hep3B cancer cells. Their growth inhibition against Hep3B cancer cells showed $19.6{\pm}4.5\%,\;54.5{\pm}6.1\%,\;96.3{\pm}2.4\%$ at 5,000 ppm, respectively. These result suggest that the traditional rice wine steamed ginseng will be useful product with antioxidant and antitumor effect.

본 연구는 인삼의 가공방법을 개발하고 제품을 다양화하기 위해 우리나라의 전통주인 탁주로 인삼을 증자한 후 홍삼의 항산화성 및 아질산염 소거능, 항암효과를 비교 연구하였다. $A1\~~A9$시료의 $60\%$ 에탄올 추출물에서의 DPPH 라디칼 소거효과는 1,000 ppm에서 인삼의 증자 횟수와 비례하여 증가하였고, 특히 A3에서 $79.5{\pm}3.3\%$로 라디칼 소거율이 뚜렷하게 증가함을 보였다. 아질산염 소거능은 pH 1.2에서 W.G는 $25.9{\pm}4.4\%$, R.G는 $12.9{\pm}1.1\%$, $A1\~A9$는 $26.2{\pm}0.1\~56.1{\pm}0.5\%$로 증자 횟수가 증가할수록 높은 아질산염 소거능을 나타냈다. 인체유래 간암세포(Hep3B)에서의 항암효과로 실험에서 W.G는 에탄올 추출물 5,000 ppm으로 처리했을 때, $19.6{\pm}4.5\%$로 나타나 증식 억제율이 미미하였으나, R.G는 에탄을 추출물 5,000 ppm으로 처리했을 때 $54.5{\pm}6.1\%$를 나타냈으며, A9에서는 $96.3{\pm}2.4\%$로 높은 항암효과 가 관찰되었다. 이상의 결과로 볼 때 탁주를 이용한 증자홍삼이 일반 홍삼 및 백삼보다 본 실험의 항산화 및 항암 system(in vitro)을 이용하여 실험한 결과 더 높은 항암 및 항산화 효과를 나타내었다.

Keywords

References

  1. Hu SY. 1976. The genus Panax (Ginseng) in Chinese medicine. Economic Botany 30: 11-28 https://doi.org/10.1007/BF02866780
  2. Kwak HS, Joo CN. 1980. Effect of ginseng saponin fraction on ethanol metabolism in rat liver. Korean J Ginseng Sci 12: 76-81
  3. Takagi K. 1974. Pharmacological studies in ginseng. Proc. 1st Intl. Ginseng Symp. Seoul, Korea. p 119-127
  4. Kim ND, Han BH, Lee EB, Kong JY. 1979. Studies on ginseng on antistress effects. Korean J Pharmacog 10: 61-65
  5. Kim MJ, Jung NP. 1987. The effect of ginseng saponin fractions on mouse immune system. Korean J Ginseng Sci 11: 130-135
  6. Park HW, Kim SC, Jung NP. 1988. The effect of ginseng saponin factions on humoral immunity of mouse. Korean J Ginseng Sci 12: 63-68
  7. Saito H, Lee YM. 1978. Pharmacological properties of panax ginseng root. Proc. 2nd Intl. Ginseng Symp, Seoul, Korea. p 109-114
  8. Park MG. 1996. Korean ginseng. Korea ginseng and tobacco research institute, Daejeon, Korea
  9. Han BH, Park MW, Woo LK, Woo WS, Han YN. 1978. Studies on the antioxidant components of Korean ginseng. Proc. 2nd Intl. Ginseng Symp., Seoul, Korea. p 13-15
  10. Yammamoto M, Hyashi Y, Kumagai A. 1980. Lipid metabolism and several saponin principles. Proc. 3rd Intl. Ginseng Symp., Seoul, Korea. p 115-116
  11. Shin HR. 2000. The cancer-preventive potential of Panax ginseng a review of human and experimental evidence. Cancer Causes and Control 11: 565-576 https://doi.org/10.1023/A:1008980200583
  12. Yun TK. 1996. Experimental and epidemiological evidence of the cancer-preventive effects of Panax ginseng C.A. Meyer. Nutr Rev 54: S71-81 https://doi.org/10.1111/j.1753-4887.1996.tb03822.x
  13. Chen X, Liu H, Lei X, Fu Z, Li Y, Tao L, Han R. 1998. Cancer chemopreventive and therapeutic activities of red ginseng. J Ethnopharmacol 60: 71-78 https://doi.org/10.1016/S0378-8741(97)00133-5
  14. Kim ND, Kang SY, Park JH, Schini-Kerth VB. 1999. Ginsenoside $Rg_3$ mediates endothelium-dependent relation in response to ginsenosides in rat aorta: role of $K^+$ channels. Eur J Pharmacology 367: 41-49 https://doi.org/10.1016/S0014-2999(98)00898-X
  15. Kim ND, Kang SY, Kim MJ, Park JH, Schini-Kerth VB. 1999. The ginsenoside $Rg_3$ evokes endothelium-independent relaxation in rat aortic rings: role of $K^-$ channels. Eur J Pharmacology 367: 51-57 https://doi.org/10.1016/S0014-2999(98)00899-1
  16. Lee SR, Park JH, Choi KJ, Kim ND. 1997. Inhibitory effects of ginsenoside $Rg_3$ on platelet aggregation and its mechanism of action. Korean J Ginseng Sci 21: 132-140
  17. Lee KY, Lee YH, Kim SI, Park JH, Lee SK. 1997. Ginsenoside Rg5 suppresses cyclin E-dependent protein kinase activity via up-regulating p21 cip/WAF and down-regulating cyclin E in sk-Hep-1 cells. Anticancer Research 17: 1067-1072
  18. Kim SE, Lee YH, Park JH, Lee SK. 1999. Ginsenoside Rs4, a new type of ginseng saponin concurrently induces apoptosis and selectvely elevatates protein levels of p53 and p21 WAFI in human hepatoma sk- Hep-l cells. Eur J Cancer 35: 507-511 https://doi.org/10.1016/S0959-8049(98)00415-8
  19. Kim SE, Lee YH, Park JH, Lee SK. 1999. Ginsenoside Rs3, a new dial-type ginseng saponin, selectvely elevatates protein levels of p53 and p21 WAFI leading to introduction of apoptosis sk-Hep-l cells. Anticancer Research 19: 487-492
  20. Kim CS, Choi KJ, Yang JW, Kim SB. 2000. Effect of preheating condition of raw ginseng on the yeild and physical property of Korean red ginseng extract. Korean J Medicinal Crop Sci 8: 146-150
  21. Park SY, Jung I, Kang TL, Park MK. 2001. Difference between steaming and decocting ginseng. J Ginseng Res 25: 37-40
  22. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH. 2000. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod 63: 1702-1704 https://doi.org/10.1021/np990152b
  23. Ro JS, Hwang SY, Hwang BH. 2001. Quantitative determination of 5-hydroxymethyl-2-furaldehyde in the Rehmanniae Radix Preparata samples at various processing stages. Kor J Pharmacogn 32: 116-120
  24. Lee KS, Ze KR, Hong SY, 1990. Studies on the extraction quantity of the specific components of crude drug preparation based on prescription. National Institute of Health 27: 326-331
  25. Park NK, Kim SL, Hur HS, Park CH. 2002. Development of R. Radix preparata with new variety 'Jiwhang 1'. Kor J Intl Agri 14: 34-39
  26. Lee JH, Koh JA, Hwang EY. 2002. Quantitative determination of 5-hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to various processings. Kor J Herbology 17: 145-149
  27. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26: 1198
  28. Choi JH, Oh SK. 1983. Studies on the anti-agiging action of Korean ginseng. Korean J Food Nutr 12: 323-335
  29. Kato H, Lee lE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formmation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  30. Kim DS, Ahn BW, Teum DM, Lee DB, Kim SB, Park YH. 1987. Degration of carcinogenic nitrisamine formation factor by natural food components: Nitrite-scavenging effects of vegetable extracts. Bull Korean Fish Soc 20: 463-468
  31. Kim DC, Chang SM, Choi J. 1995. Variation of effective constituents contents, physical properties and color intensities of extracts from white ginseng roots of different cultivating years. Agric Chem Biotechnol 38: 67-71
  32. 中林敏朗, 木村進, 加藤博通 1967. 食品の變色とその化學. 光琳書院, 東京. p 223-289
  33. Do JH, Kim KH, Jang JG, Yang JW, Lee KS. 1989. Changes in color intensity and components during browning reaction of white ginseng water extract. Korean J Food Sci Technol 21: 480-485
  34. Shank RC. 1975. Toxicology of N-nitro compounds. Toxicol Appl Pharmacol 31: 361-368 https://doi.org/10.1016/0041-008X(75)90257-4
  35. Kim SB, Lee DH, Yeum DJ, Park JW, Park YH. 1988. Nitrite scavengin effect of mailard reaction products derived from glucose-amono acids. Korean J Food Sci Technol 20: 453-458
  36. Choi DY. 1999. Effect of growth inhibition in Hep3B cell and HeLa cell by treatment of Euonymus alatus (Thunb.) Sieb extracts. Dongguk J Institute of Oriental Medicine 7: 155-162

Cited by

  1. A Study on the Saponin Contents and Antioxidant Activity of the Ginseng and Extruded Ginseng by Using Different Solvents for Extraction vol.24, pp.4, 2011, https://doi.org/10.9799/ksfan.2011.24.4.528
  2. Analysis of Microbial Diversity in Makgeolli Fermentation Using PCR-DGGE vol.22, pp.2, 2012, https://doi.org/10.5352/JLS.2012.22.2.232
  3. 한약재 포제 가공의 현대적 연구 현황 vol.39, pp.4, 2005, https://doi.org/10.4333/kps.2009.39.4.275