한국가금학회지 (Korean Journal of Poultry Science)

  • 제32권2호
  • /
  • Pages.135-141
  • /
  • 2005
  • /
  • 1225-6625(pISSN)
  • /
  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
권호 표지

한국재래 수탉에서 부화 후 혈청내 LH, Testosterone, Estrogen과 IGF-I 농도의 변화

Changes in the Profiles of Serum LH, Testosterone, Estrogen and IFG-I during Sexual Development in Male Korean Native Chickens

  • 태현진 (전북대학교 생체안전성연구소, 수의과대학 해부학교실) ;
  • 장병귀 (축산연구소 가금과) ;
  • 최철환 (축산연구소 가금과) ;
  • 박영재 (전북대학교 생체안전성연구소, 수의과대학 해부학교실) ;
  • 양홍현 (전북대학교 생체안전성연구소, 수의과대학 해부학교실) ;
  • 김인식 (전북대학교 생체안전성연구소, 수의과대학 해부학교실)
  • Tae H. J. (Bio-Safety Research Institute, Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Jang B. G. (Poultry Division National Livestock Research institute) ;
  • Choi C. H. (Poultry Division National Livestock Research institute) ;
  • Park Y. J. (Bio-Safety Research Institute, Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Yang H. H. (Bio-Safety Research Institute, Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University) ;
  • Kim I. S. (Bio-Safety Research Institute, Department of Veterinary Anatomy, College of Veterinary Medicine, Chonbuk National University)
  • 발행 : 2005.06.01
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초록

한국재래 자에서 부화 후부터 성숙에 이르는 시기까지 황체형성호르몬 자극에 대한 고환내 testosterone 생성과 혈청내 황체형성호르몬, estradiol, IGF-I 및 testosterone 농도의 변화를 알아보기 위하여 부화 후 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 28, 32, 44, 52 및 64주령(n=13마리/일령)의 한국재래 닭을 이용하여 방사면역측정법을 적용하여 이 연구를 수행하였다. 혈청내 estradiol의 농도는4주령과 비교하여 8, 12, 16, 21, 32 및 44주령에서는 차이가 없었으나 52주령과 64주령에서는 큰 폭으로 증가하였다. 혈청내 황체 형성호르몬의 농도는 $1\~12$주령까지는 유의성이 없었고 14주령부터 32주령까지 점진적으로 증가한 다음 유의성 있게 감소하였으며 32주령에 최고치와 2주령에 최저치를 나타내었다. 혈청내 IGF-I의 농도는 $1\~16$주령까지는 유의성 있게 증가하였으나 이후에는 변화가 없이 낮게 유지되었다. 혈청내 testosterone농도는 1, 2, 4 및 8주령에는 유의성이 없고 $10\~32$주령까지 유의성있게 증가하였으며 $24\~32$주령 및 $32\~64$주령에서는 유의성이 없었다. 황체형성호르몬 자극에 대한 고환내 testosterone 생성은 $1\~32$주령까지 유의성 있게 증가하였고 $44\~64$주령까지는 큰 폭으로 감소하였다.

Changes in serum estradiol, insulin-like growth factor-1, leuteinizing and testosterone levels, and leuteinizing hormone-stimulated testosterone production per testis in vitro from hatching to adulthood were studied in Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The changes in the profiles of the levels in the incubation medium of luteinizing hormone-stimulated (100 ng/mL) testosterone secretion per testis in vitro, and the serum LH, testosterone, estradiol, and insulin-like growth factor-I were determined by radioimmunoassay. Serum estradiol levels were not significantly different at week 4 compared to that of 8, 12, 16, 21, 32, :md 44. Significant decreases were observed at weeks 52 and 64. Serum leuteinizing hormone concentrations were not significantly different from 1 week to 12 weeks, increased gradually up to 32 weeks of age, and declined significantly thereafter; the highest value was at 32 weeks, and the lowest value was detected at 2 weeks of age. Serum insulin-like growth factor-I concentrations increased significantly from 1 week to 16 weeks, remained low and unchanged with advancing age. Serum testosterone concentrations were not significantly different at week 1 compared weeks 2, 4, 6, and 8. Significant increases were observed from 10 weeks to 32 weeks of age. Values at weeks 24, 28 and 32 and at weeks 32, 44, 52, and 64 were not significantly different. The highest value was at weeks 28 and the lowest value was detected at weeks 1 week. LH-stimulated testosterone production per testis in vitro increased gradually with age from 1 to 32 weeks and decreased significantly from 44 weeks to 64 weeks of age.

키워드

참고문헌

  1. Barenton B, Pelletier J 1983 Seasonal changes in testicular gonadotropin receptors and steroid content in the ram. Endocrinology 112:1441-1446 https://doi.org/10.1210/endo-112-4-1441
  2. Benahmed M, Morera AH, Chauvin MC 1987 Somatomedin C/insulin-like growth factor- I as a possible intratesticular regulation of Leydig cell activity. Mol Cell Endocrine 50:69-77 https://doi.org/10.1016/0303-7207(87)90078-5
  3. Berfield AK, Spicer D, Abrass CK 1997 Insulin-like growth factor I (IGF-I) induces unique effects in the cytoskeleton of cultured rat glomerular mesangial cells. J Histochem Cytochem 45:583-593 https://doi.org/10.1177/002215549704500410
  4. Clay CM, Squires EL, Amann RP, Pickett BW 1987 Influences of season and artificial photoperiod on stallions : testicular size, seminal characteristics and sexual behavior. J Anirn Sci 64:517-525 https://doi.org/10.2527/jas1987.642517x
  5. Clegg EJ 1966 Pubertal growth in the Leydig cells and accessory reproductive organs of the rat. J Anat 100:369-379
  6. Cohen-Parsons H, Van Krey HP, Siegel PB 1983 In vivo aromatization of eHJtestosterone in high and low mating lines of Japanese quail. Horm Behav 17: 316-323 https://doi.org/10.1016/0018-506X(83)90031-4
  7. Connell CJ 1972 The effect of luteinizing hormone on the ultrastructure of the Leydig cell of the chick. Z Zellforsch 128:139-151 https://doi.org/10.1007/BF00306894
  8. Craven RP, Clarke JR 1982 Gonadotrophin levels in male voles(Microtus agrestis) reared in long and short photoperiods. J Reprod Fert 66:709-714 https://doi.org/10.1530/jrf.0.0660709
  9. Daughaday WH, Rotwein P 1989 Insulin-like growth factors I and II . Peptide messenger ribonucleic acid and gene structure serum, and tissue concentrations. Endor Rev 10:68-91 https://doi.org/10.1210/edrv-10-1-68
  10. De Mellow JSM, Handelsman DJ, Baxter RC 1987 Short-term exposure to insulin-like growth factors stimulates testosterone production by testicular interstitial cells Acta Endocr 115: 483-49
  11. Desjardins C 1981 Endocrine signaling and male reproduction. Bioi Reprod 24:1-21 https://doi.org/10.1095/biolreprod24.1.1
  12. Eroschenko VP, Wilson WD, Siope TD 1977 Function and histology of testes from aged Cotumix maintained on di- fferent photoperiods. J Gerontol 32:279-285 https://doi.org/10.1093/geronj/32.3.279
  13. Foidart A, DeClerck A, Harada N, Balthazart J 1994 Aromataseimmunoreactive cells in the quail brain-effects of testosterone dad sex dimorphism. Physiol Behav 55: 453- 464 https://doi.org/10.1016/0031-9384(94)90100-7
  14. Handelsman DJ, Spaliviero JA, Scott CD, Baxter RC 1985 Identification of insulin-like growth factor-I and its receptors in the rat testis. Acta Endocr 109:543-549
  15. Lam F, Farner DS 1976 The ultrastructure of cells of leydig in the white crowned sparrow (Zonotrichia leucophrys gambelii) in re1atio to plasma levels of luteinizing hormone and androgen. Cell Tiss Res 169:93-109
  16. Lee CY, Henricks DM 1990 Comparisions of various acidic treatments of bovine serum on insulin-like growth factor-1 immunoreactive and binding activity. J Endocrinol 127:139-148 https://doi.org/10.1677/joe.0.1270139
  17. Lipsett MB 1980 Physiology and pathology of the Leydig cell. New Engl J Med 303: 682-688 https://doi.org/10.1056/NEJM198009183031207
  18. Mashaly MM, Kratzer KR, Keene OD 1983 Effect of photoperiod on body weight and reproductive performance of ringneck pheasants. Poult Sci 62:2109-2113 https://doi.org/10.3382/ps.0622109
  19. Muncher Y, Sod-Moriah VA, Weill S, Rosenstrauch A, Friedlander, M 1995 Intratesticular retention of sperm and premature decline in fertility in the domestic rooster, Gallus domesticus. J Exp Zool 273:76-81 https://doi.org/10.1002/jez.1402730110
  20. Ottinger MA, Brinkley HJ 1978 Testosterone and sex related behaviour and morphology: Relationships during maturation in the adult Japanese quail. Horm Behav 11: 175-182 https://doi.org/10.1016/0018-506X(78)90046-6
  21. Rohss M, Silverin B 1983 Seasonal variation in the ultrastructure of Leydig cells and plasma levels of luteinizing hormone and steroid hormone in juvenile and adult male great tits Parus major. Omis Scandinavia 14:202-212 https://doi.org/10.2307/3676154
  22. Rosenstrauch A, Degen AA, Bedrad E, Friedlander M 1994a Improvement of fertility in cornish roosters by the use of clomiphene citrate. Pages 1025-1028 in Larbier, M. (ed.) 7th European Poultry Conference
  23. Rosenstrauch A, Degen AA, Friedlander M 1994b Spermatozoa retention by sertoli cells during the decline in fertiliy in aging roosters. BioI Reprod 50:129-136 https://doi.org/10.1095/biolreprod50.1.129
  24. Rozenboim I, Dgany O, Robinzon B, Amon E, Snapir N 1989 The effect of tamoxifen on the reproductive traits in white leghorn cockerels. Pharmacol Biochem Behav 32: 377-381 https://doi.org/10.1016/0091-3057(89)90166-4
  25. Sharpe PJ, Gow CB 1993 Neuroendocrine control of reproduction in the cockerel. Poult Sci 62:1671-1675
  26. Sinha-Hikim AP, Amador AG, Bartke A, Russell LD 1989 Structure/function relationship in active and inactive hamster Leydig cells : A correlative morphometric and endocrine study. Endocrinology 125:1844-1856 https://doi.org/10.1210/endo-125-4-1844
  27. Suttie JM, White RG, Breier BH 1991 Photoperiod associated changes in Insulin-like growth factor-I in reindeer. Endocrinology 129:679-682 https://doi.org/10.1210/endo-129-2-679
  28. Tahka KM 1986 Current aspects of Leydig cell function and its regulation. J Reprod Fert 78:367-380 https://doi.org/10.1530/jrf.0.0780367
  29. Tahka KM, Rajaniemi H. 1985 Photoperiodic modulation of testicular LH receptors in the bank vole( Clethrionomys glareolus). J Reprod Fert 75:513-519 https://doi.org/10.1530/jrf.0.0750513
  30. Tsui HW, Tam WH, Lofts B, Phillips JG 1974 The annual testicular cycle and androgen production in vitro in the masked civet cat, Paguma L. Larvata. J Reprod 36:283-293 https://doi.org/10.1530/jrf.0.0360283
  31. Wilson JD, George FW, Griffin JE 1981 The hormonal control of sexual development. Science 211: 1278-1284 https://doi.org/10.1126/science.7010602