International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Cloning and Characterization of Ribosome-associated Membrane Protein 4 (RAMP4) gene in silkworm Bombyx mori

  • Yao Qin (Institute of Life Sciences, JiangSu University) ;
  • Hu Zhigang (Institute of Life Sciences, JiangSu University) ;
  • Xu Jiaping (Institute of Life Sciences, JiangSu University) ;
  • Chen Keping (Institute of Life Sciences, JiangSu University)
  • Published : 2005.06.01
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Abstract

Ribosome-associated membrane protein 4 (RAMP4) is a membrane protein that exposes its N-terminal hydrophilic portion on the cytoplasmic side and spans the membrane close to the C-terminal end. RAMP4 has previously been reported to belong to the set of proteins that remains associated with membrane-bound ribosomes, and controls the glycosylation of major histocompatbility complex class II-associated invariant chain. RAMP4 also may be relative to the stabilization of membrane proteins in response to stress, with other components of translocon, and molecular chaperons in ER. Application of 5'-RACE technique with specially designed primer, we cloned a 715 bp cDNA fragment which contains a 195 bp ORF, termed RAMP4. The deduced protein has 64 amino acid residues and contains a putative transmembrane-spanning domain at the COOH terminus.

Keywords

References

  1. Bendtsen, J. D., N. Henrik, V. H. Gunnar and S. Brunak (2004) Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340, 783-795 https://doi.org/10.1016/j.jmb.2004.05.028
  2. Florea, L., G. Hartzell, Z. Zhang, G. M. Rubin and W. Miller (1998) A computer program for aligning a cDNA sequence with a genomic DNA sequence. Genome Res. 8, 967-974
  3. Gorlich, D. and T. A. Rapoport (1993) Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. Cell 75, 615-630 https://doi.org/10.1016/0092-8674(93)90483-7
  4. Hartmann, E., T. A. Rapoport and H. F. Lodish (1989) Predicting the orientation of eukaryotic membrane-spanning proteins. Proc. Natl. Acad. Sci. USA 86, 5786-5790 https://doi.org/10.1073/pnas.86.15.5786
  5. Kutay, U., E. Hartmann and T. A. Rapoport (1993) A class of membrane proteins with a C-terminal anchor. Trends Cell Biol. 3, 72-75 https://doi.org/10.1016/0962-8924(93)90066-A
  6. Lu, H. S. (1998) Molecular Biology of Insect Viruses. pp 1-15, Agricultural Scientech Press, Beijing
  7. Milanesi, L., M. Muselli and P. Arrigo (1996) Hamming Clustering method for signals prediction in 5'and 3'regions of eukaryotic genes. Comput. Applic. Biosci. 12, 399-404
  8. Rahman, M. M. and K. P. Gopinathan (2004) Systemic and in vitro infection process of Bombyx Mori nucleopolyhedrovirus. Virus Res. 101, 109-118 https://doi.org/10.1016/j.virusres.2003.12.027
  9. Reese, M. G. (2001) Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genome. Comput. Chem. 26, 51-56 https://doi.org/10.1016/S0097-8485(01)00099-7
  10. Reese, M. G., F. H. Eeckman, D. Kulp and D. Haussler (1997) Improved splice site detection in Genie. J. Comp. Biol. 4, 311-23 https://doi.org/10.1089/cmb.1997.4.311
  11. Schroder, K., B. Martoglio, M. Hofmann, C. Holscher, E. Hartmann, S. Prehn, T. A. Rapoport and B. Dobberstein (1999) Control of glycosylation of MHC class II-associated invariant chain by translocon-associated RAMP4. EMBO J. 18, 4804-4815 https://doi.org/10.1093/emboj/18.17.4804
  12. Wada, I., M. Kai, S. Imai, F. Sakane and H. Kanoh (1997) Promotion of transferrin folding by cyclic interaction with calnexin and calreticulin. EMBO J. 16, 5420-5432 https://doi.org/10.1093/emboj/16.17.5420
  13. Wang, L. and B. Dobberstein (1999) Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum. FEBS Lett. 457, 316-322 https://doi.org/10.1016/S0014-5793(99)01075-3
  14. Yamaguchi, A., O. Hori, D. M. Stem, E. Hartmann, S. Ogawa, and M. Tohyama (1999) Stress-associated Endoplasmic Reticulum Protein 1 (SERP1)/Ribosome-associated Membrane Protein 4 (RAMP4) Stabilizes Membrane Proteins during Stress and Facilitates Subsequent Glycosylation. J. Cell Biol. 147, 1195-1204 https://doi.org/10.1083/jcb.147.6.1195