International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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cDNA Cloning and Developmental Expression of Hemolin in Bombyx mandarina

  • Kang Min Uk (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim Kyung-A (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee Jin Sung (Research Institute of Life Science & Biotech, CoreBio) ;
  • Kim Nam Soon (Human Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang Seok-Woo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Nho Si-kab (College of Agriculture and Life Sciences, Kyungpook National University)
  • Published : 2005.06.01
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Abstract

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

Keywords

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