• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.2
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• pp.101-106
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• 2005

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.75-75
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• 2003

1. To compare a full cDNA sequence of hemolin, a bacteria-induced protein of lepidopteran insects, from 4 geographically different strains of Bombyx mori. 2. To determine developmental profiles of hemolin gene expression in Bombp mori. (omitted)

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2001.11a
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• pp.109-109
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• 2001

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.208.2-208
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• 2001

No Abstract, See Full Text

• Proceedings of the Korean Society of Applied Entomolohy Conference
• /
• 2002.11a
• /
• pp.110-110
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• 2002

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.104-104
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• 2005

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2004.04a
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• pp.38-38
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• 2004

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.19-24
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• 1996

To investigate the genes which is related to immune reaction of Bombyx mori, differential screening was carried out using naive and induced B. mori mRNA probe. To begin with, we constructed the cDNA library with mRNA isolated from fifth instar larvae injected with E. coli(4 X 106 cells/larva) using Uni ZAP XR vector kit. Thirty-two inducible cDNAs showing higher intensity on the induced mRNA probing membranes were selected. Partial nucleotide sequences of 29 clones were determined and their expessed sequence tags (ESTs) were produced. Nineteen ESTs in 29 ESTs were matched in GenBank database and the rest of them were found to be unknown. These unmatched ESTs were presumed to be novel genes. The nineteen ESTs contained variable genes related to biological process in Bombyx mori and four classes immune genes. Four clones, BmInc 6, 8, 18 and 27 were similar to two antibacterial peptide genes, hemolin gene and transferrin gene, respectively.