Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antiplatelet Activity of Thujopsis dolabrata var. hondai-Derived Component Against Platelet Aggregation

  • SON DONG JU (College of Natural Sciences, Soonchunhyang University) ;
  • PARK YOUNG HYUN (College of Natural Sciences, Soonchunhyang University) ;
  • KIM YOUNG MI (Research Center for Industrial Development of Biofood Materials and Faculty of Biotechnology, College of Agriculture, Chonbuk National University) ;
  • CHUNG NAM HYUN (College of Life and Environmental Sciences, Korea University) ;
  • LEE HOI SEON (Research Center for Industrial Development of Biofood Materials and Faculty of Biotechnology, College of Agriculture, Chonbuk National University)
  • Published : 2005.04.01
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Abstract

The steam distillate obtained from Thujopsis dolabrata var. hondai sawdust was fractionated by centrifugal thin-film evaporation, and the fractions were then investigated for antiplatelet activity using washed rabbit platelets. The biologically active constituent of T. dolabrata var. hondai sawdust was isolated by silica gel column and HPLC chromatographies and characterized as carvacrol by various spectral analyses. Carvacrol inhibited platelet aggregation induced by collagen, arachidonic acid, and platelet activating factor with IC$_{50}$ values of 12.6, 2.5, and 385.3 $\mu$M, respectively. However, carvacrol had no effect on thrombin, calcium ionophore A23l87, or phorbol l2-myristate l3-acetate induced platelet aggregation. Carvacrol was a much more potent inhibitor, as antiplatelet agents, compared with aspirin. These results suggest that carvacrol isolated from T. dolabrata var. hondai sawdust may be useful as a lead compound for inhibiting arachidonic acid-induced platelet aggregation.

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References

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