Lactobacillus spp., Bifidobacterium spp. 및 Bacillus coagulans의 과산화수소 저항성과 과산화수소 생성 능력

Resistant Activity to Hydrogen Peroxide of Lactobacillus spp., Bifidobacterium spp., Bacillus coagulans and Hydrogen Peroxide Generation Capability of Lactobacillus spp.

과산화수소 형태의 반응성 산소에 대한 Lactobacillus Spp., Bifidobacterium spp. 및 Bacillus coagulans의 저항성과 세포 내에서 생성능력을 측정하기 위하여 본 연구가 수행되었다. Lactobacillus spp. 중에서 높은 과산화수소 저항성을 나타낸 균주는 L. acidophilus CU4111와 L. casei Cu4114인 것으로 나타났고 가장 낮은 저항성을 보인 균주는 L. brevis Cu4206이었다. Bifidobacterium longum CU4131은 높은 수준의 저항성을 나타내며 Bacillus coagulans를 포함하는 포자형성유산균주들은 전반적으로 과산화수소에 대한 저항성 이 높은 것으로 나타났다. 세포질 추출액 중의 과산화수소 함유 농도는 Bifidobacterium bifidum CU 4134가 가장 높고 Lactobacilli spp.의 세포질 추출액 중의 과산화수소 함유 농도는 L. casei CU 4114가 가장 높은 것으로 나타났다.

Studies on the resistance of Lactobacillus ssp., Bifidobacterium spp. and Bacillus coagulans to hydrogen peroxide were conducted by determination of the viable cells after the test cells in 2mM hydrogen peroxide solution for a predetermined time; L. acidophilus CU4111 and L. casei CU4114 were most resistant to the hydrogen peroxide among the fifteen test lactobacilli strains, whereas L. brevis Cu4206 was the strain which was the most susceptible to hydrogen peroxide. Bifidobacterium longum Cu4131 was one of the resistant strains. A prominant tendency found out that Bacillus coagulans possessed a strong resistance to hydrogen peroxide. The results of level of hydrogen peroxide determination in the cell extracts showed all the test strains contained hydrogen peroxide in the cytoplasm, the amount varied depending on the strain and species of lactic acid bacteria. Bifidobacterium bifidum CU 4134 and L. casei CU 4114 were potent hydrogen peroxide producer strain.