Morphological and Genetic Diversity of Korean Native and Introduced Safflower Germplasm

  • Shim Kang-Bo (Upland & Industrial Crop Division, National Yongnam Agricultural Research Institute, RDA) ;
  • Bae Seok-Bok (Upland & Industrial Crop Division, National Yongnam Agricultural Research Institute, RDA) ;
  • Lim Si-Kyu (Upland & Industrial Crop Division, National Yongnam Agricultural Research Institute, RDA) ;
  • Suh Duck-Yong (Upland & Industrial Crop Division, National Yongnam Agricultural Research Institute, RDA)
  • 발행 : 2004.09.01

초록

Morphological and genetic diversity of thirty nine safflower germplasm were collected and evaluated by Principal Component Analysis (PCA) and Random Amplified Polymorphic DNA (RAPD) method. Stem length and seeding to flowering days of the safflower germplasm showed $26\~117cm\;and\;76\~179$ days of variation respectively. USA originated germplasm showed higher oil content as $39\%$, but that of Japanese showed lower as $26\%$. PCA made three different cluster groups according to some agronomic characteristics of safflower. Korea originated germplasm showed similar cluster group with that of collected from USA in the PCA of stem length. But in the seeding to flowering days, it showed similar cluster pattern with that of collected from Japan rather than USA. In the experiment of RAPD analysis, total five primers showed polymorphism at the several chromosomal loci. Korea, China Japan and South Central Asia originated germplasm were differently classified with USA and South West Asia originated germplasm with lower similarity coefficient value (0.47). Most of Korea originated germplasm were grouped with South Central Asia originated germplasm with higher similarity coefficient value (0.74) conferring similar genetic background between both of them. China and Japan originated germplasm were dendrogramed with Korea originated germplasm at the 0.65 and 0.50 similarity coefficient values respectively. Some common results were expected from both of PCA and RAPD analysis, but lower genetic heritability caused by relative higher portion of environmental variance and environment by genotype interaction at the expression of those of agronomic characteristics made constraint to find any reliable results.

키워드

참고문헌

  1. An, D K and C S Yuk 1975 Present medecnial plants In : Safflower Kommon Publishers 358-359
  2. Cooper, M. and I H Delacy 1994 Relationships among analytical methods used to study genotype variation and genotype by environment interaction in plant breeding multi-environment experiments The Appl Genet. 88 561-572 https://doi.org/10.1007/BF01240919
  3. Jonshon, R. C ,W J Johnston, C. T. Golob, M. C. Nelson, and R J. Soreng. 2002 Characterization of the USDA poa pratensis collection using RAPD markers and agronomic descriptors Genetic Resources and Crop Evolution49 : 349-361
  4. Kee, C. H. 1993 The pharmacology of chinese herbs CRC, press 249
  5. Kim, J. H., D. T. Kwak, D Y Choi, and K D. Moon. 1999 Comparison of the chemical compositions of korean and chinese safflower seed Korean J. Food Sci Technology 31(4) . 912-918
  6. Lee, C. B 1980 Picture Book of Korean Plants In . Safflower. Baekyang Publishers. 779-800
  7. Nei, W. and W H Li. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Nat'l Acad Sci 76: 5269-5273 https://doi.org/10.1073/pnas.76.10.5269
  8. Park, S. W. 1984 Studies on cultural practice and useful composition of korean local safflower Ph.D. thesis Univ. of Konkuk, Seoul, Korea
  9. Williams, J G K, A R Kubelik, K. J Livak , J A. Rafalski, and S V. Tingey 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic acids Res 18: 6531-6532 https://doi.org/10.1093/nar/18.22.6531
  10. Yan, W. and L A Hunt 1998. Genotype by environment intraction and crop yield Plant Breed. Rev. 16 135-178