Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Characteristics of HIV-Tat Protein Transduction Domain

  • Yoon Jong-Sub (Department of Microbiology, The Catholic University of Korea) ;
  • Jung Yong-Tae (Department of Microbiology, College of Advanced Science, Dankook University) ;
  • Hong Seong-Karp (Catholic Hemopoietic Stem Cell Transplantation Center, The Catholic University of Korea) ;
  • Kim Sun-Hwa (Department of Microbiology, The Catholic University of Korean) ;
  • Shin Min-Chul (Department of Microbiology, The Catholic University of Korean) ;
  • Lee Dong-Gun (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Shin Wan-Shik (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Min Woo-Sung (Department of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Paik Soon-Young (Department of Microbiology, The Catholic University of Korea)
  • Published : 2004.12.01
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Abstract

The human immunodeficiency virus type 1 (HIV-I) Tat protein transduction domain (PTD), which con­tains rich arginine and lysine residues, is responsible for the highly efficient transduction of protein through the plasma membrane. In addition, it can be secreted from infected cells and has the ability to enter neighboring cells. When the PTD of Tat is fused to proteins and exogenously added to cells, the fusion protein can cross plasma membranes. Recent reports indicate that the endogenously expressed Tat fusion protein can demonstrate biodistribution of several proteins. However, intercellular transport and protein transduction have not been observed in some studies. Therefore, this study exam­ined the intercellular transport and protein transduction of the Tat protein. The results showed no evi­dence of intercellular transport (biodistribution) in a cell culture. Instead, the Tat fusion peptides were found to have a significant effect on the transduction and intercellular localization properties. This sug­gests that the HIV-1 PTD passes through the plasma membrane in one direction.

Keywords

References

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