차 카테킨 EGCG (Epigallocatechin Gallate)의 구강세균에 대한 살균효과

Effect of Tea Catechin, EGCG (Epigallocatechin Gallate) on Killing of Oral Bacteria

  • 유미옥 (순천향대학교 자연과학대학 생명과학부) ;
  • 천재우 (순천향대학교 자연과학대학 생명과학부) ;
  • 오계헌 (순천향대학교 자연과학대학 생명과학부)
  • Yu Mi-Ok (Department of Life Science, Soonchun­hyang University) ;
  • Chun Jae-Woo (Department of Life Science, Soonchun­hyang University) ;
  • Oh Kye-Heon (Department of Life Science, Soonchun­hyang University)
  • 발행 : 2004.12.01

초록

본 연구는 차 카테킨인 EGCG (epigallocatechin gallate)에 의한 구강세균의 살균효과를 조사하기 위하여 수행되었다. 초기 세포밀도 $10^{7}$ cell/ml의 대상 세균에 대한 항세균 활성은 EGCG 1ml당 5mg에서 조사하였다. 선택 또는 복합배지상에서 집락생성단위(colony-forming unit, CFU)에 근거하여 EGCG의 항세균 활성은 농도에 비례하는 것을 확인하였다. Streptococcus mutans와 Streptococcus sobrinus는 5mg/ml의 EGCG에서 8시간 이내에 완전히 살균되었다. Lactobacillus acidophilus와 Lactobacillus plantarum도 동일한 조건에서 각각 2시간과 4시간 이내에 살균되었다. 2.5 mg/ml의 EGCG로 처리한 사람의 타액에서 유래하는 총 구강세균의 생존율을 BHI 고체배지상에서 조사하였으며, 총 구강세균은 10시간 이내에 완전히 살균되었다. MS 고체배지상에서 S. mitis and S. salivarius의 집락은 감소되었으며 배양 12시간 이내에서는 집락은 관찰되지 않았다. 그 결과, EGCG는 입냄새와 치석의 원인이 되는 구강세균을 살균시키고 충치를 예방하는 자연적이며 효과적인 물질임이 입증되었다.

The purpose of this work was to investigate the effect oftea catechin, epigallocatechin gallate (EGCG) on killing of oral bacteria. The antibacterial activity of 2.5 mg/ml and 5.0 mg/ml EGCG was investigated for target bacteria of which initial cell number was approximately adjusted to $10^{7}ml$. The antibacterial activity of EGCG was proportional to the concentration according to colony-forming unit(CFU) of target bacteria enumerating on selective and complex media. Streptococcus mutans and Streptococcus sobrinus at 5mg/ml EGCG were completely killed within 8 hrs. Lactobacillus plantarum and Lactobacillus acidophilus were also killed within 2 hrs and 4 hrs under the same conditions, respectively. Oral bacteria at 2.5 mg/ml EGCG were completely killed within 10 hr. Colony numvers of S. mitis and S. salivarius treated with 2.5 mg/ml EGCG were decreased on MS solid media and no colony was observed on the media within 12 hrs. In consequence, EGCG would be a natural and effective compound that kill oral bacteria being caused of bad breath, plaque and gingivitis, and for preventing and treating dental caries.

키워드

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