Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cyclosophoraose as a Novel Chiral Stationary Phase for Enantioseparation

  • JUNG, YUN-JUNG (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University) ;
  • LEE, SANG-HOO (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University) ;
  • PAIK, SEUNG-R. (School of Chemical Engineering, Seoul National University) ;
  • JUNG, SEUN-HO (Department of Microbial Engineering and Bio/Molecular Informatics Center, Konkuk University)
  • Published : 2004.12.01
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Abstract

Cyclosophoraoses (Cys), cyclic ${\beta}-(1{\rightarrow}2)-D-glucans$ produced by Rhizobium meliloti 2011, were used as a novel chiral stationary phase for the enantiomeric separation. A novel Cys stationary phase, chemically immobilized onto porous silica via aminopropyltrimethoxysilane as a molecular linker, showed good separation for each racemate of bupivacain (separation factor, $\alpha$=1.3), propranolol ($\alpha$=1.3), and fenoprofen ($\alpha$=2.9), respectively, under the mobile phase of water: methanol (80:20, v/v) at a constant flow rate of 0.9 ml/min at pH7.

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References

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