Journal of Microbiology and Biotechnology

  • 제14권6호
  • /
  • Pages.1333-1337
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  • 2004
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

The Effect of Overexpression of Rat Clusterin in L929 Fibroblasts

  • PARK , JUNG-HYUN (Experimental Immunology Branch, National Cancer Institute) ;
  • JU, SUNG-KYU (School of Bioscience and Biotechnology, Chungnam National University) ;
  • PARK, JEE-SUN (School of Bioscience and Biotechnology, Chungnam National University) ;
  • PARK, YOO-KYOUNG (Department of Food and Nutrition, Hannam University) ;
  • KANG, MYUNG-HEE (Department of Food and Nutrition, Hannam University) ;
  • YOU, KWAN-HEE (School of Bioscience and Biotechnology, Chungnam National University)
  • 발행 : 2004.12.01
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초록

Oxidants such as hydrogen peroxide are powerful inducers of cell damage, ageing, and apoptosis. Since clusterin, a 75-80 kDa mammalian glycoprotein, is frequently found to be inducible in apoptotic cells and tissues, this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidant­induced stress and apoptosis. To clarify this issue, we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides, clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand, neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed, which leaves the question open about its anti-apoptotic mechanism. In conclusion, the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.

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참고문헌

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