대한치과보철학회지 (The Journal of Korean Academy of Prosthodontics)

  • 제42권3호
  • /
  • Pages.301-306
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  • 2004
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  • 0301-2875(pISSN)
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  • 2005-3789(eISSN)
대한치과보철학회 (The Korean Academy of Prosthodonitics)
권호 표지

ADSORPTION OF HUMAN RECOMBINANT FIBRONECTIN TO TITANIUM IN VITRO

  • Kim H.J. (Department of Prosthodontics, College of Dentistry, Seoul National University) ;
  • Chang I.T. (Department of Prosthodontics, College of Dentistry, Seoul National University) ;
  • Koak J.Y. (Department of Prosthodontics, College of Dentistry, Seoul National University) ;
  • Heo S.J. (Department of Prosthodontics, College of Dentistry, Seoul National University) ;
  • Yim S.A. (Department of Prosthodontic dentistry, Samsung Medical Center, School of Medicine, Sungkyunkwan University) ;
  • Jang J.H. (Intellectual Biointerface Engineering Center, Seoul National University College of Dentistry)
  • 발행 : 2004.06.01
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초록

Statement of problem. Fibronectin mediates its biological effects by binding to integrins on cell membranes through a consensus site including the Arg-Gly-Asp (RGD) sequence within tenth type III module. Purpose. The purpose of our study was to investigate the adsorption affinity of human recombinant fibronectin peptide (hFNIII 9-10) to titanium and to investigate the effect of the surrounding ionic composition on the adsorption process. Material and methods. As for evaluating the affinity of hFNIII 9-10 to Ti, titanium disks were incubated in 40, 80 and $120{\mu}g/ml$ hFNIII 9-10 solution at $37^{\circ}C$ overnight, repectively. As for evaluating the effect of surrounding ionic concentration, hFNIII 9-10 was dissolved in distilled water, phosphate buffered saline and RPMI 1640. Optical density (O.D.) was measured in ELISA reader. Results. The results were as follows; 1. The adsorption of hFNIII 9-10 showed significantly highest mean optical density (O.D.) value in $80{\mu}g/ml$. 2. The difference of ionic composition in DW, PBS and RPMI did not influence the adsorption amount of hFNIII 9-10.

키워드

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