Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Differential Display Detection of Acid-inducible Genes from Porphyra yezoensis Thalli

해조류 방사무늬김 (Porphra yezoensis) 엽체로부터 산 유도 유전자의 분리

  • JIN Long-Guo (Department of Biotechnology, Pukyong National University) ;
  • KANG Se-Eun (Department of Biotechnology, Pukyong National University) ;
  • CHOI Jae-Suk (Department of Biotechnology, Pukyong National University) ;
  • PARK Sun-Mee (Department of Biotechnology, Pukyong National University) ;
  • PARK Jung-Youn (Biotechnology Division, National Fisheries R&D Institute) ;
  • JIN Duck-Hee (Faculty of Marine Biotechnology, Kangnung National University) ;
  • HONG Yong-Ki (Department of Biotechnology, Pukyong National University)
  • ;
  • 강세은 (부경대학교 생물공학과) ;
  • 최재석 (부경대학교 생물공학과) ;
  • 박선미 (부경대학교 생물공학과) ;
  • 박중연 (국립수산과학원 생물공학과) ;
  • 진덕희 (강릉대학교 해양생물공학부) ;
  • 홍용기 (부경대학교 생물공학과)
  • Published : 2004.08.01
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Abstract

Genetic responses of the edible seaweed Porphyra yezoensis tissue to acid shock have been compared using differential display technique. The tissue was challenged in seawater containing $0.05{\%}$ hydrogen chloride (pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs. Total RNA extracted by the LiCl-guanidium method was reverse transcribed and amplified by PCR with arbitrary primers. The amplified fragment responded by the acid shock was selectively isolated from agarose gel and sequenced with DNA auto sequencer. Sequence (1056 bp) of the cDNA contained at least two genes for ASP7K (MW 7418) and ASP5K (MW 5512) proteins.

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References

  1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology, Green Publishing Associates and Wiley-Interscience, New York, pp. 414-416
  2. Chung, Y.K., J.I. Choi and G.J. Kim. 1992. Studies on the control of diseases and diatoms on laver using mixed solutions of organic acids and trace metals. Bull. Natl. Fish. Res. Dev. Agency, 96, 215-226
  3. Chung, Y.K., S.K. Cho and G.J. Kim. 1989. Studies on the control of the Licmophora abbreviata on laver leaves. Bull. Natl. Fish. Res. Dev. Agency, 73, 180- 195. (in Korean)
  4. Critchley, A.T. and M. Ohno. 1998. Seaweed Resources of the World. Japan International Cooperation Agency. Japan. pp. 431
  5. Garbarino, J.E., D.R. Rockhold and W.R. Belknap. 1992. Expression of stress-responsive ubiquitin genes in potato tubers. Plant Mol. Biol., 20, 235-244 https://doi.org/10.1007/BF00014491
  6. Gong, Y.G., S.C. Cheong, D.Y. Lee, S.W. Kim, J.W. Chang, Y.G. Jeong, J.I. Choi, H.G. Kim and C.K. Lee. 1992. Studies on the control of fouling organism on the laver Porphyra. Bull. Natl. Fish. Res. Dev. Agency, 96, 178-191. (in Korean)
  7. Hong, Y.K., C.H. Sohn, M. Polne-Fuller and A. Gibor. 1995. Differential display of tissue-specific messenger RNAs in Porphyra perforata (Rhodophyta) thallus. J. Phycol., 31, 640-643 https://doi.org/10.1111/j.1529-8817.1995.tb02560.x
  8. Kawasaki, E.S. 1990. Amplification of RNA. In: PCR Protocols: A Guide to Methods and Applications, Innis, M.A., D.H. Gelfand, J.J. Sninsky and T.J. White, eds. Academic Press, San Diego, pp. 21-27
  9. Liang, P. and A.B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the poly- merase chain reaction. Science, 257, 967-971 https://doi.org/10.1126/science.1354393
  10. Nam, B.H., H.J. Jin, S.K. Kim and Y.K. Hong. 1998. Quantitative viability of seaweed tissues assessed with 2,3,5-triphenyltetrazolium chloride. J. Appl. Phycol., 10, 31-36 https://doi.org/10.1023/A:1008059314639
  11. Park, J.W., Y.C. Cho, B.H. Nam, H.J. Jin, C.H. Sohn and Y.K. Hong. 1998. RAPD identification of genetic variation in the seaweed Hizikia fusiformis. J. Mar. Biotechnol., 6, 62-64
  12. Park, Y.J., S.K. Kim, G.J. Kim and S.K. Cho. 1990. Studies on the control of the green algae, Enteromorpha linza from the cultivating purple-laver net. Bull. Natl. Fish. Res. Dev. Agency, 44, 77-85. (in Korean)
  13. Payvar, F. and R.T. Schimke. 1979. Methylmercury hy- droxide enhancement of translation and transcription of ovalbumin and conalbumin mRNAs. J. Biol. Chem., 254, 7636-7642
  14. Provasoli, L. 1968. Media and prospects for cultivation of marine algae. In: Cultures and Collections of Algae, Watanabe, A. and A. Hattori, eds. Jap. Soc. Plant Physiol., pp. 63-75
  15. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York, pp. 18.88
  16. Yu, K. and K.P. Pauls. 1992. Optimization of the PCR program for RAPD analysis. Nucl. Acids Res., 20, 2606 https://doi.org/10.1093/nar/20.10.2606