Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Effects of Dichloromethane fraction of Phlomodis Radix(DFPR) on differentiation of Mouse Calvarial Cell

속단의 dichloromethane 분획물이 마우스 두개골 세포의 분화에 미치는 영향

  • Kim, Dong-Jin (Department of periodontology, College of Dentistry, Yonsei University) ;
  • Yun, Jeong-Ho (Department of periodontology, College of Dentistry, Yonsei University) ;
  • Jung, Ui-Won (Department of periodontology, College of Dentistry, Yonsei University) ;
  • Yoo, Yun-Jung (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Kim, Yun-Chul (Department of Pharmacy, Pharmacy College, Wonkwang University) ;
  • You, Hyung-Keun (Department of periodontology, School of Dentistry, Wonkwang University) ;
  • Kim, Chong-Kwan (Department of periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical science) ;
  • Choi, Sung-Ho (Department of periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical science)
  • 김동진 (연세대학교 치과대학 치주과학교실) ;
  • 윤정호 (연세대학교 치과대학 치주과학교실) ;
  • 정의원 (연세대학교 치과대학 치주과학교실) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실) ;
  • 김윤철 (원광대학교 약학대학) ;
  • 유형근 (원광대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학 교실, BK21 의과학 사업단) ;
  • 최성호 (연세대학교 치과대학 치주과학 교실, BK21 의과학 사업단)
  • Published : 2004.12.31
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Abstract

The purpose of this study was to evaluate the effects of DFPR on differentiation of mouse calvarial cell in vitro, to examine the possibility for periodontal regeneration. $10{\mu}g/ml$ of DFPR was used as experimental concentration. osteogenic medium only was assigned as control, Experimental 1 was supplemented with 10nM dexamethasone, Experimental 2 with $10{\mu}g/ml$ DFPR and Experimental 3 with l0nM dexamethasone + $10{\mu}g/ml$ DFPR. cellular activity was evaluated by MTT method at 8, 12, 16 days, expression of mRNA of ALP, osteopontin, osteocalcin, collagen type-l was detected by RT-PCR method at 4, 8, 12, 16 days of culture. extent of mineralization was observed by Von Kossa staining at 16 day of culture. The results are as follows 1)Any acceleration of differentiation was not observed at expression of differentiation marker, 2) Decrease in expression of extracelluar matrix and in bone nodule formation was observed The results suggested that DFPR have negative effect on the rate of differentiation on rat calvarial cell, decrease extracelluar matrix formation ,decrease bone nodule formation. Ongoing studies are necessary in order to determine effect of DFPR on periodontal regeneration.

Keywords

References

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