Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Effects of Calcium Phosphate Glass on Mouse Calvarial Cell

Calcium Phosphate Glass가 마우스 두개골 세포에 미치는 영향

  • Kim, Min-Kyoung (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Chang-Sung (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Lee, Doug-Youn (Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University) ;
  • Lee, Yong-Keun (Department and Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University) ;
  • Cho, Kyoo-Sung (Department of Periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical Science) ;
  • Chai, Jung-Kiu (Department of Periodontology, College of Dentistry, Yonsei University) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical Science) ;
  • Choi, Seong-Ho (Department of Periodontology, College of Dentistry, Yonsei University, Brain Korea 21 Project for Medical Science)
  • 김민경 (연세대학교 치과대학 치주과학교실, 치주조직 재생연구소) ;
  • 김창성 (연세대학교 치과대학 치주과학교실, 치주조직 재생연구소) ;
  • 이덕연 (연세대학교 치과대학 치과생체재료학교실) ;
  • 이용근 (연세대학교 치과대학 치과생체재료학교실) ;
  • 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소, BK21 의과학 사업단) ;
  • 채중규 (연세대학교 치과대학 치주과학교실, 치주조직 재생연구소) ;
  • 김종관 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소, BK21 의과학 사업단) ;
  • 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소, BK21 의과학 사업단)
  • Published : 2004.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

The goal of periodontal treatment is not only to arrest the progression of the disease but also to promote the functional, esthetic regeneration of the periodontium. Flap operation, bone graft, guided tissue regeneration, growth factors and bone morphogenetic protein have been used for this purpose. Among these techniques of regeneration, alloplastic graft, especially calcium phosphate is getting more attention recently. The purpose of this study was to evaluate the effects of calcium phosphate glass on mouse calvarial cell in vitro. The toxicity of calcium phosphate glass was measured using MTT assay, the synthesis of collagen was measured using collagen assay, and ALP activity was measured. The experimental groups were cultured with calcium phosphate glass(both AQ-, and HT-CPG) in concentration of 0.01, 0.02, 0.1, 0.2g/ml. The results are as follows 1. In concentrations not exceeding 0.02g/ml, both the groups(AQ-CPG, HT-CPG) didn't show any toxicity on mouse calvarial cell(p<0.05). 2. In both the experimental groups are the concentration of 0.02g/ml, collagen expressions were significantly up-regulated (p<0.05). 3. In both the experimental groups are the concentration of 0.02g/ml, ALP activity was not significantly up-regulated, but ALP activity in both experimental groups were greater than control group(p<0.05). The results suggested that the use of calcium phosphate glass may promotes periodontal regeneration. Ongoing studies are necessary in order to determine their regeneration effects.

Keywords

References

  1. Caton JG, Quinones CR : Etiology of periodontal disease. Curr Opin Dent 1: 17-28, 1991
  2. Froum SJ, Gomez C: Periodontal regeneration. Curr Opin Periodontol 64: 744-753, 1993
  3. Brunsvold MA, Mellonig JT: Bone grafts and periodontal regeneration. Periodontol 2000 1:80-91, 1983
  4. Dragoo MR, Sullivan HC: A clinical and histological evaluation of autogenous iliac bone grafts in humans II. External root resorption. J Periodontol 44: 614-625, 1973
  5. Quintero G, Mellonig JT: A six months clinical evaluation of decalcified freeze-dried bone allo-graft in periodontal osseous defects. J Periodontol 53: 726-730, 1982
  6. Cecilia Y, Peter S, Annika S:A comparative study of anorganic xenogenic bone and autogenous bone implants for bone regeneration in rabbits. Int J Oral Maxillof Implant 14: 72-76, 1999
  7. Froum SJ, Kushner L, Scopp Stahl SS: Human clinical and histologic response to durapatite implants in intraosseus lesions. J Periodontol 57: 7-13, 1986
  8. Ogose A, Hotta T, Hatano H, Kawashima H, Tokunaga K, Endo N, Umezu H: Histologic examination of beta-tricalcium phosphate graft in human femur. J Biomed Mater Res 63(5): 601-604, 2002
  9. Yukna RA: Clinical evaluation of HTR polymer and freeze-dried bone allograft. A case report. J Clin Periodontol 23: 615-620, 1996
  10. Zamet JS, Dabar UR, Griffiths GS, Bulman JS, Bragger U, Burgin W, Newman HN: Particulate bioglass as a grafting material in treatment of periodontal intrabony defects. J Periodontol 24: 410-418, 1997
  11. Louise F, Borghetti A: Clinical evaluation of natural coral implantation in osseous periodontal defects. Results after one year. J Periodontol 10: 69-76,1991
  12. Higashi S, Ohsumi T, Ozumi K, Kuroki K, Inokuchi Y, Terashita M: Evaluation cytotoxicity of calcium phosphate cement consisting of ${\beta}$tricalcium phosphate and dicalcium phosphate dihydrate. Dent Mater J 17: 186-194, 1998
  13. Yoshimine Y, Sumi M, Isobe R, Anan H, Maeda K: In vitro interaction between tetracalcium phosphate-based cement and calvarial osteogenic cells. Biomaterials 17: 2241-2245, 1996
  14. Bowers GM, Vargo JW, Levy B, Emerson JR, Bergquist JJ: Histologic observations following the placement of tricalcium phosphate implants in human intrabony defects. J Periodontol 57(5): 286-287, 1986
  15. Hashimoto UM, Ishikawa I, Kinoshita A, Weng HT, Oda S: Clinical and histologic observation of replacement of biphasic calcium phosphate by bone tissue in monkeys. Int J Periodontics Restorative Dent 15(2): 205-213, 1995
  16. Stahl SS, Froum SJ: Histologic evaluation of human intraosseus healing response to the placement of tricalcium phosphate ceramic implants. J Periodontol 57: 211-217, 1986
  17. Shahmiri S, Singh IJ, Stahl SS: Clinical response to the use of HTR polymer implant in human infrabony lesions. Int J Periodontics Restorative Dent 12: 294-299, 1992
  18. Trisi P, Rao W, Rebaudi A, Fiore P: Histologic effect of pure-phase beta-tricalcium phosphate on bone regeneration in human artificial jaw-bone defects. Int J Periodontics Restorative Dent 23(1): 69-77, 2003
  19. Yukna RA: Synthetic bone grafts in periodontics. Periodontol 2000 1: 92-99,1993
  20. Garret S: Periodontal regeneration around natural teeth. Ann Periodontol 1: 621-666, 1996
  21. Ratner BK, Hoffman AS, Schoen FJ, Lemons JE, Hench LL: Biomaterial science. Academic press. 1996
  22. Yamamuro T, Hench LL, Wilson J, Davies JF, Brady J: Handbook of Bioactive ceramics 2. CRCc Press, 1990
  23. Andriano KP, Daniels AJ: Biocompatibility and mechanical properties of a totally absorbable composite material for orthopedic fixation devices. J Appl Biomat. 3: 197-206, 1992
  24. Gross JM, Lickfield G, Latour RA: Calcium phosphate reinforced bioabsorbable polymer composites. Prec 23rd meeting of biomat 159,1997
  25. 양승민, 설양조, 계승범, 이인경, 김석영, 이용무, 구영, 한수부, 정종평, 최상묵, 류인철: 다양한 형태의 다공질 calcium phosphate의 생분해성에 관한 장기적인 연구. 33(2): 301-305, 2003
  26. Lee YK, Lin S, Rohanizadeh R, eGeros JP, LeGeros RZ: Preparation and properties of calcium phosphate glasses obtained from the system CaO-$CaF_{2}-P_{2}O_{5}$-MgO-ZnO. J Biomed mater Res, In Press
  27. 이용근, 문현주, 이상배, 김광만, 김경남, 최성호: 치주조직 재생을 위한 칼슘 포스페이트 글라스 분말이 백서 두개골 결손부의 골조직 재생에 미치는 영향. 대한 치과기재학회지 30(2): 153-161, 2003
  28. Lee YK, Kim HS, Cho KS, Kim KN, Choi SH: Investigation of bone formation using non-crystalline calcium phosphate glass in beagle dogs. Key Engineering Materials: 391-394, 2003
  29. Mosmann T: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63, 1983
  30. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47(4): 936-42, 1987
  31. Hugoson A, Laurell L, Lundgren D: Frequency distribution of individuals aged 20-70 years according to severity of periodontal disease experience in 1973 & 1983. J Clin Periodontol. 19(4): 227-232, 1992
  32. Saffar JL, Colombier ML, Detienvile R: Bone formation in tricalcium phosphate filled periodontal intrabony lesions. J Periodontol 61: 209-216, 1990
  33. Nefussi JR: Sequential expression of bone matrix proteins during rat calvaria osteoblast differentiation and bone nodule formation in vitro. J Histochem Cytochem 45: 493-503, 1997
  34. Ramakrishnan PR, Lin WL, Sodek J, Cho MI: Synthesis of noncollagenous extracellular matrix protein during development of mineralized nondules by rat periodontal ligament cells in vitro. Calcif Tissue Int 57: 52-59, 1995
  35. Roth JA, Kim BG, Lin WL, Cho MI: Melatonin promotes osteoblast differentiation and bone formation. J Bio Chem 274: 22041-22047, 1999
  36. Bellows CG, Aubin JE, Heersche JN: Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate. Bone and Mineral 14(1): 27-40, 1991
  37. Woldarski KH, Reddi AH:Alkaline phosphase as a marker of osteoinductive cells. Calcif Tissue Int 39: 382-385, 1986
  38. Arceo N, Sauk JJ, Moehring J, Foster RA, Somerman MJ: Human periodontal cells initiate mineral-like nodules in vitro. J Periodontol 62: 499-503, 1991
  39. Ohgushi H, Dohi Y, Yoshikawa T, Tamai S, Okunaga K, Shibuya T: Osteogenic differentiation of cultured marrow stromal stem cells on the surfaces of bioactive glass ceramics. J Biomed Mater Res 32(3): 341-348, 1996
  40. Viornery C, Guenther H, Aronsson B, Pechy P, Descouts P, Gratzel M: Osteoblast culture on polished titanium disks modified with phosphonic acids. J Biomed Mater Res 62: 149-155, 2002