Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Di-2-ethylhexyl Phthalate Induced Haematological Effects in Bagrid Catfish, Pseudobagrus fulvidraco After Short Term Exposure

Di-2-ethylhexyl Phthalate (DEHP)에 노출된 동자개, Pseudobagrus fulvidraco의 혈액적

  • Jee, Jung-Hoon (Institute of Fisheries Sciences, Pukyong National University) ;
  • Keum, Yoo-Hwa (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • 지정훈 (부경대학교 수산과학연구소) ;
  • 금유화 (부경대학교 수산생명학과) ;
  • 강주찬 (부경대학교 수산생명학과)
  • Published : 2004.09.30
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Abstract

Di-2-ethylhexyl phthalate (DEHP) is a widely used plasticizer known to be a suspected xenoestrogen and a causative agent of oxidative damage to the RBC cell membrane ir vitro. We evaluated the toxic effects of a scarcely documented aquatic environmental pollutant, DEHP, on selected haematological endpoints in the bagrid catfish, Pseudobagrus fulvidraco. Bagrid catfish were exposed to DEHP (300, 1,000 mg DEHP kg body weight$^{-1}$) through thrice intraperitoneal injection and effects were assessed in blood of the exposed organisms. Haematological property, serum organic and inorganic chemistry were monitored in blood of Bagrid catfish. DEHP exposed-fish showed erythropenia; low hematocrit (Ht) and hemoglobin (Hb) content and red blood cell count showed a significantly higher than in that of the control group. The treatment group showed a significantly lower concentration of serum total protein, cholesterol and triglyceride compared with those in the control group. The value of calcium and osmolality were significantly decreased in the DEHP treatment group, compared with the control group.

Di-2-ethylhexyl Phthalate (DEHP)는 내분비장애물질로 분류되어 있는 플라스틱 가소제로서 in vitro에서 혈구세포막에 영향을 주는 것으로 알려져 있다. 본 연구는 동자개, Pseudobagrus fulvidraco를 대상으로 DEHP 급성 노출에 따른 혈액학적 영향을 파악하기위하여 어체중 당 300 및 1,000 mg의 DEHP를 복강주사하였다. DEHP (1,000 mg b.w. $^{-1}$에 노출된 동자개는 적혈구수와 혈색소 농도 및 적혈구 용적이 감소하는 경향을 나타내었다. 또한, 혈청 내 유기성분인 총단백질량, 콜레스테를 수치는 300 및 1,000 mg 주사구에서 유의적으로 감소하였으며 지방산의 농도는 1,000 mg 주사구에서 대조구와 비교하여 유의적으로 감소하였다. 혈청 무기성분인 칼슘농도와 혈액 삼투압 농도는 1,000 mg DEHP 노출구에서 유의적인 감소가 관찰되었다.

Keywords

References

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