Potential in the Application for Biological Control of Harmful Algal Bloom Cased by Microcystis aeruginosa

유해성 조류 Microcystis aeruginosa의 생물학적 제어를 위한 미소생물제재의 적용 실험

  • Kim, Baik-Ho (Department of Life Science, Hanyang University) ;
  • Choi, Hee-Jin (Department of Environmental Science, Hanyang University) ;
  • Han, Myung-Soo (Department of Life Science, Hanyang University)
  • 김백호 (한양대학교 자연과학대학 생명과학과) ;
  • 최희진 (한양대학교 자연과학대학 환경과학과) ;
  • 한명수 (한양대학교 자연과학대학 생명과학과)
  • Published : 2004.03.31

Abstract

Growth inhibition of Microcystis aeruginosa was examined with single-or mixed treatment of algicidal bacterium Streptomyces neyagawensis and heterotrich ciliate Stentor roeseli, which isolated from natural freshwater. The harmful Cyanobac-terium, Microcystis aeruginosa density was effectively suppressed by the algicidal bacterium Streptomyces neyagawensis, and the bacterial biomass was few changed. The heterotrich ciliate S, roegeji isolated from the eutrophic Pal'tang riverine, Korea suppressed the algal biomass effectively. But mixed-treatment of both bio-agents was less effective, leading to an increase in algal density.

부영양호수의 남조 Microcystis aeruginosa 제어를 위해 호수 바닥층에서 분리한 살조세균과 섬모충을 현장여과수를 이용하여 조류배양조건과 동일한 조건에서 단일 또는 혼합적용하여 그 결과를 서로 비교하였다. 두 생물제재는 저자들의 선행연구와 같이 단일 적용시 매우효과적인 반면, 혼합적용시 오히려 조류의 성장을 촉진하였다. 결국 남조 Microcystis제어를 위한 섬모충이나 박테리아의 적용은 다른 한 생물군의 낮은 밀도를 요구하였으며, 이처럼 동일 조류에 대한 제어능을 갖는 두 생물제재의 배타적인 관계는 앞으로 부영양호수의 남조대발생 제어에 귀중한 자료로서 제공될 것이다.

Keywords

References

  1. 나은경, 신경숙, 장재헌, 강 호. 2003. 전자선조사를 이용한 부영양화 호수의 조류제어에 관한 연구. 대한환경공학회 2003 춘계학술연구발표대회. KAIST 2003.5.1-3. p. 504-509
  2. 문은영. 2002. 팔당호의 섬모충플랑크톤의 분류 및 생태학적 연구. 한양대 석사논문
  3. 박혜경. 2000. 녹조현상. 수질분야-환경관련미생물 21. 환경자료집 vol. 2
  4. 신정이, 박석순. 2001. 하천 수생식물의 영양염류 제거능 산정 에 관한 연구. 한국물환경학회지 17: 201-213
  5. 이정호, 박종근, 김은정. 2002. 국내 주요 호수의 식물플랑크톤종조성 및 영양단계 평가. Algae 17: 275-281
  6. 장은희, 김정동, 한명수. 2003. 남조류 분해세균 HY0210-AK1 의 분리와 특성 및 Arabaena cylindrica 분해 활성. 한국환경생물학회지 21: 194-202
  7. 전만식, 김범철. 1999. 부레옥잠의 수중영양염 제거 잠재력에 관한 고찰. 한국환경물학회지 17: 117-124
  8. Ahn, C.Y., M.H. Park, S.H. Joung, H.S. Kim, K.Y. Jang and H.M. Oh. 2003. Growth inhibition of cyanobacteria by ultrasonic radiation: Laboratory and enclosure studies. Environ. Sci. Technol. 37: 3031-3037
  9. Bettarel, Y., C. Amblard, T. Sime-Ngando, J.F. Carrias, D. Sargos, F. Garabetian and P. Lavandier. 2003. Viral lysis, flagellate grazing potential, and bacterial production in Lake Pavin. Microbial. Ecology. 45: 119-127
  10. Burnham, J.C., S.A. Collart and M.J. Daft. 1984. Myxococcal predation of the cyanobacterium Phormidium luridum in aqueous environments. Arch. Microbiol. 137:220-225
  11. Carmichael, W.W. 1988. Toxins of freshwater algae. In Tu AT (Ed.) Handbook of Natural Toxins, Vol. 3, Marine Toxins, and Venoms. Marcel Dekker, New York, p. 121-47
  12. Cha, G.C. and M.G. Hwang. 2001. Nitrogen removal and behavior of soluble microbial products (SMP) in the MBR process with intermittent aerobic condition. Kor. Membrane Journal 3: 1-8
  13. Choi, H.J., J.D. Kim, B.H. Kim, J.W. Lee and M.S. Han. 2004. Does Isolated Streptomyces neyagawaensis control the bloom of Microcystis aeruginosa? Submitted
  14. Daft, M.J., S.B. McCord and W.D.P. Stewart. 1975. Ecological studies on algal-lysing bacteria in fresh waters. Freshwater Biol. 5: 577-596
  15. Daggett, P.M. and A. Nerad. 1992. Protocols in protozoology. The Society of Protozoologists, A-42
  16. Dawson, R.M. 1998. The toxicology of microcystins. Toxicon. 36: 953-962
  17. Foissner, W. and S. Wolfi. 1994. Revision of the genus Stentor Oken (Protozoa, Ciliophora) and description of S. araucanus nov. spec. from South American lakes. J. Plankton Res. 16: 255-289
  18. Fukami, K., A. Yuzawa, T. Nishijima and Y. Hata. 1992. Isolation and properties of a bacterium inhibition the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi 58: 1073-1077
  19. Fukushima, M., N. Takamura, B.H. Kim, M. Nakagawa, L. Sun and Y. Zheng. 2000. The responses of an aquatic ecosystem to the manipulation of the filter-feeding silver carp (Hypophthalmichthys molitrix) Verh. Internat. Verein. Limnol. 27: 1-7
  20. Gosselain, V., J.-P. Descy, L. Virous, C. Joaquim-Justo, A. Hammer, A. Metens and S. Schweitzer. 1998. Grazing by large river zooplankton: a key to summer potamoplankton decline? The case of the Meuse and Moselle rivers in 1994 and 1995. Hydrobiologia 369/370: 199- 216
  21. Kang, Y.H., J.D. Kim, B.H. Kim, D. Kong and M.-S. Han. 2004. Identification of bacterium HYK0203-SK02 and it’s lysis of Stephanodiscus hatzschii. Submitted
  22. Kasai, F., N. Takamura and S. Hatakeyama. 1993. Effects of simetyrene on growth of various freshwater algal taxa. Environ Pollu. 79: 77-83
  23. Kim, B.H. and M.S. Han. 2003. Usage of bio-agents to control cyanobacterial and diatomal bloom in Pal’tang reservoir, a Korean freshwater. Spring Conference of Korean Society of Limnology, Ecology, and Environ-mental Biology, P 7~8, June 20~21, Taegu University, Taegu, Korea
  24. Kim, B.H., M.K. Choi and N. Takamura. 2003. Phytoplankton preferences of young silver carp, Hypophthalmichthys molitrix, in hypereutrophic mesocosms during a warm season. J. Freshwater Ecol. 18: 69-77
  25. im, C.J., I.K. Lee, B.S. Yun and I.D. Yoo. 1993. Concanamycin B, active substrate against Phytophthora capsici produced by Streptomyces neyagawaensis 38D10 strain. Kor. J. Appl. Microbiol. Bioeng. 21: 322-328
  26. Kim, J.D. and M.-S. Han. 2003. Identification of alga-lytic bacterium AK-07 and its enzyme activities associated with degradability of cyanobacterium Anabaena cylindrica. Kor. J. Limnol. 36: 108-116
  27. Manage, M.P., Z. Kawabata and S. Nakano. 2000. Algicidal effect of the bacterium Alcaligenes denitrificans on Microcystis spp. Aquat, Microb. Ecol. 22: 111-117
  28. Nicolau, A., N. Dias, M. Mota and N. Lima. 2001. Trends in the use of protozoa in the assessment of wastewater treatment. Research in microbiology 152: 621-630
  29. Ohki, K. and Y. Fujita. 1996. Occurrence of a temperate cyanophage lysogenising the marine cyanophyte Phormidium persicum. J. Phycol. 32: 365-370
  30. Park, M.H., C.Y. Ahn, B.D. Yoon and H.M. Oh. 2003. Growth inhibition of Microcystis aeruginosa by rice straw extract. Submitted
  31. Parker, D.L., H.D. Kumar, L.C. Rai and J.B. Singh. 1997. Potassium salts inhibit growth of the Cyanobacteria Microcystis spp. in pond water and defined media:implications for control of Microcystin-producing aquatic blooms. Appl. Environ. Microbiol. 63: 2324-2329
  32. Pendergrass, W.R. 1980. Carolina protozoa and invertebrates manual. Carolina biological supply company, 13pp
  33. Redhead, K. and S.J. Wright. 1978. Isolation and properties of fungi that lyse blue-green algae. Appl. Environ. Microbiol. 35: 962-969
  34. Reynolds, C.D. 1985. The ecology of freshwater phytoplankton. Cambridge Univ Press. 384pp
  35. Shimaz, A., C.J. Kim and I.D. Yoo. 1993. Diversity of Actinomycetes-Species, morphology and life cylcle- Kor. J. Appl. Microbiol. Biotechnol. 21: 88-94
  36. Shinya, K., I. Akiko, M. Atsushi and M. Masahoro. 2002. Isolation and identification of the antialgal compound, harmane (1-methyl-β-carboline), produced by the algicidal bacterium, Pseudomonas sp. K44-1. J. Appl.Phycol. 14: 109-114
  37. Sigee, D.C., R. Glenn, M.J. Andrews, E.G. Bellinger, R.D. Butler, H.A.S. Epton and R.D. Hendry. 1999. Biological control of cyanobacteria: principles and possibilities. Hydrobiologia 395/396: 161-172
  38. Tartar, V. 1961. The biology of Stentor. Pergamon Press, 413pp
  39. Vincent, W.F. 1987. Dominance of blooming forming cyanobacteria (blue-green). New Zea, J. Mar. Freshwater Res. 21: 361-542
  40. Williams, S.T., S. Lanning and E.M.H. Wellington. 1984. Ecology of actinomycetes, p. 481-528. In The biology of the actinomycetes. M. Goodfellow, M. Mordarski, and S.T. Williams (ed), Academic Press, London, U.K
  41. Wright, S.J., K. Redhead and H. Maudsley. 1981. Acanthamoeba castellanii, a predator of cyanobacteria. J. Gen. Microbiol. 125: 293-300
  42. Wu, J.T., L.L. Kuo-Huang and J. Lee. 1998. Algicidal effect of Peridinium bipes on Microcystis aseruginoa. Current Microbiology 37: 257-261
  43. Yamamoto, Y. 1981. Observations on the occurrence of microbial agents which cause lysis of blue-green algae in Lake Kasumigaura. Jpn. J. Limnol. 42: 20-27
  44. Yamamoto, Y., T. Kouchiwa, Y. Hodoki, K. Hotta, H. Uchida and K. Harada. 1998. Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake. J. Appl. Phycol. 10: 391-397