Journal of Korean Ophthalmic Optics Society (한국안광학회지)
- Volume 9 Issue 1
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- Pages.173-180
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- 2004
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- 1226-5012(pISSN)
The comparative study on Cell Cytotoxicity of H2O2 and Grapefruit Seed extract
콘택트렌즈 보존제 H2O2와 자몽씨 추출물의 세포 독성 비교 연구
- Kim, In-Suk (Department of Ophthalmic Optics, Chodang university) ;
- Yoo, Geun-Chang (Department of Ophthalmic Optics, Chodang university)
- Received : 2004.02.28
- Published : 2004.04.24
Abstract
This study aims to investigate the effects of hydrogen peroxide and grapefruit seed extract used as a chemical and natural disinfectants on human conjunctival cells in vitro. The main component of grapefruit seed extract is a narigin. It is one of the flavonoid types in citrus fruits and f1avonoids are widely recognized as naturally occurring(삭제) antioxidants. Cytotoxicity was determined by mitochondrial activity(MTT assay) and DNA damage was analyzed by measuring Comet assay. In LDH assay, 5% of grapefruits seed extract has been observed as a material is giving recovery effect of damaged cultured conjuctival cells by hydrogen peroxide. And also, each of concentrations has been treated simultaneously with same amounts and cytotoxicity of hydrogen peroxide and grapefruit seed extract have been estimated by LDH leakage assay after 24 hours. In conclusion, H2O2-induced cytotoxicity, apoptosis were Significantly prevented by grapefruit seed extract. It is a main component of bioflavonoids that we can simply take it as food. The present results suggest that grapefruit seed extract is a useful disinfectanct having antioxidant and antiapoptopic activity as a natural product.
콘택트렌즈 관리용액으로 사용되는 합성보존제로서 대표적인