Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

The Effects of Healthful Decoction Utilizing Phellinus linteus in Carbon Tetrachloride-injected Rats

상황을 이용한 한의학적 보건기능개선제에 대한 연구

  • Kang Kyung Hwa (Department of Physiology, College of Oriental medicine, Research Institute of Oriental Medicine, Dongeui University) ;
  • Lee Jun Hyuk (Department of Anatomy, College of Oriental medicine, Research Institute of Oriental Medicine, Dongeui University) ;
  • Choi Yung Hyun (Department of Biochemistry, College of Oriental medicine, Research Institute of Oriental Medicine, Dongeui University) ;
  • Choi Byung Tae (Department of Anatomy, College of Oriental medicine, Research Institute of Oriental Medicine, Dongeui University) ;
  • Lee Yang Tae (Department of Physiology, College of Oriental medicine, Research Institute of Oriental Medicine, Dongeui University)
  • 강경화 (동의대학교 한의과대학 생리학교실ㆍ한의학연구소) ;
  • 이준혁 (동의대학교 한의과대학 해부학교실ㆍ한의학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실ㆍ한의학연구소) ;
  • 최병태 (동의대학교 한의과대학 해부학교실ㆍ한의학연구소) ;
  • 이용태 (동의대학교 한의과대학 생리학교실ㆍ한의학연구소)
  • Published : 2004.02.01
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Abstract

We investigated the effects of healthful decoction utilizing Phellinus linteus(HDPL) for suppression in the process of carbon tetrachloride (CCl₄4)-induced inflammation(50% CCl₄ : olive oil=1:1, 1 ㎖/KgㆍB.W.) of rat using biochemical, Western, histological and immunohistochemical analysis. Biochemical analysis of serum showed that the level of aspartate aminotransferase, alanine aminotransferase, lactate Dehydrogenase, alkaline phosphatase and triglyceride were significantly decreased by pretreatment of HDPL, but albumin and nitric oxide were increased. Immunoblot analysis of the liver showed that CCl₄-induced expression of interleukin-1β(IL-1β) and inducible nitric oxide synthase(iNOS) was inhibited by pretreatment of HDPL. More severe histopathological changes of the liver such as Kupffer cell reaction, inflammatory cell infiltration and focal necrosis were demonstrated in the rats challenged with CCl₄ compared with normal. Fewer scores of these changes were observed in the HDPL pretreated rats. Immunohistochemical analysis of the liver showed that while the expression of IL-1β, iNOS, tumor necrosis factor-α, COX(cyclooxygenase)-1 and COX-2 tended to increase, a decline of these immunoreaction of HDPL pre-treated groups were observed in the hepatocytes, especially in the focal necrotic sites. These results suggest that HDPL may act as a therapeutic agent for liver disease through a regulation of inflammation-related proteins.

Keywords

References

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