Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Taekunyukmijiwhang-tang on Blood Pressure and Renal function in NO-dependent Hypertensive Rats

택군육미지황탕이 산화질소의존형 고혈압백서의 혈압과 신장기능에 미치는 영향

  • Sohn Eun Jin (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Kang Dae Gill (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Noh Suk Yeon (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Lee An Sook (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Yin Ming Hao (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Moon Mi Kyung (Department of Herbal Resources, Professional Graduate School of Oriental Medicine) ;
  • Yun Young Gab (College Oriental Medicine University) ;
  • Lee Ho Sub (Department of Herbal Resources, Professional Graduate School of Oriental Medicine)
  • 손은진 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 강대길 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 노숙연 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이안숙 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 윤명호 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문미경 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 윤용갑 (한의과대학 한의학과) ;
  • 이호섭 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2004.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present study examined the effects of Taekunyukmijiwhang-tang (TV) on blood pressure and renal function in nitric oxide (NO)-dependent hypertensive rats. A phamacological inhibition of nitric oxide synthase (NOS) for 4-6 weeks produces renal vasoconstriction, renal dysfunction, and progressive severe hypertension. Treatment of rats with NG-Nitro-L-arginie methylester (L-NAME) (100 mg/L, 6 weeks), which is a nonspecific NOS inhibitor, cause a sustained increase in systolic blood pressure (SBP), along with the decrease in expression of ecNOS in the kidney and thoracic aorta. The expression of Na, K-ATPase α1 subunit in the kidney was also reduced in the L-NAME induced hypertensive rats group. The renal functional parameters including urine osmolality (Uosm), creatinine clearance (Ccr), which is an index of glomerular filtration (GFR) were decreased in rat with L-NAME induced hypertension. while solute-free water reabsoption (TcH₂O) was unchanged in all experimental group. However, the group combined treated with TV and L-NAME did not develop hypertension and expression of ecNOS in the aorta was restored. The expression of Na/sup +/, K/sup +/-ATpase α1 subunit in the kidney was markedly restored in L-NAME-induced hypertension rats by administration of TV along with the restoration of urinary volume (UV) and sodium excretion (UNaV), whlie Na/sup +/, K/sup +/-ATPase /β1 subunit was not altered. These results suggest that TV attenuates an increase in SSP in the L-NAME induced hypertension and restores partially renal function, which seems to be caused by up-regulation of expression of Na/sup +/, K/sup +/-ATPase α1 subunit in the kidney and ecNOS in thoracic aorta.

Keywords

References

  1. 醫方集解 汪인庵
  2. 證補 萬病回春 공廷賢
  3. 醫學入門 李천
  4. 石室秘錄 鎭士易
  5. 中藥方濟學 梁頌名
  6. 方劑學 王錦之
  7. 方劑學 張恩勤;鄭貴力
  8. 경희한의대 논문집 v.2 육미지황탕 전탕액이 가토 혈압 및 백서 간의 TBA에 미치는 영향에 대한 연구 김우식;이동희
  9. 경희대 한의대논문집 v.7 육미지황탕이 백서의 혈당량에 미치는 영향 허종회;유가원
  10. 원광대학교 대학원 논문집 v.3 육미지황탕이 항개량형 마빈신염에 미치는 영향 유지윤
  11. 동의보감 허준
  12. Atrip report of the American Herbal phamacology delegation Herbal phamacology in the people's Republic of China
  13. Japan Society for the promotion of Science Onthe chinese medicinal plants Sato J.
  14. 申氏本草學(總論) 申佶求
  15. 本草學 李商仁
  16. 생약학회지 v.12 no.4 택사의 Alisol 성분들이 몇가지 Agonist에 대한 적출장관 수축에 미치는 영향 윤계숙;정성현;김영숙
  17. 동의심계내과학(上) 이경섭 등
  18. 대한한의학회지 v.5 no.11 고혈압에 대한 임상적 고찰 김영석
  19. Harrison's 내과학(II) Harrison
  20. Am J Physiol v.284 no.6 Role of renal NO production in the regulation of medullary blood flow Cowley AW Jr;Mori T;Mattson D;Zou AP https://doi.org/10.1152/ajpcell.00224.2002
  21. Braz J Med Biol Res v.36 no.7 Oral administration of L-arginine decreases blood pressure and increases renal excretion of sodium and water in renovascular hypertensive rats Gouvea SA;Moyses MR;Bissoli NS;Pires JG;Cabral AM;Abreu GR https://doi.org/10.1590/S0100-879X2003000700017
  22. Nephron Physiol v.95 no.2 Renal and vascular nitric oxide system in reduced renal mass saline hypertension Arranz C;Tomat A;Fellet A;Garcia J;Balaszczuk AM;Costa M https://doi.org/10.1159/000073678
  23. In Quantitative Clinical Chemistry Methods v.2 Phillips RA.;Peter JP;Vanslyke DD(Eds.)
  24. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford MM https://doi.org/10.1016/0003-2697(76)90527-3
  25. 최신임상정신의학 이근후
  26. 대한신경과학회지 v.3 no.1 치매의 병리 지제근
  27. 경희의학 v.7 최근 뇌졸중에 대한 역학적 고찰 나영설;윤상협;민병일
  28. Pathol v.99 no.1 Protective role of endothelial nitric oxide synthase Albrecht EW;Stegeman CA;Heeringa P;Henning RH;van Goor HJ
  29. Biochem Biophys Res Commun v.196 no.3 Differential expression of iNOS and cNOS mRNA in human vascular smooth muscle cells and endothelial cells under normal and inflammatory conditions MacNaul KL;Hutchinson NI https://doi.org/10.1006/bbrc.1993.2398
  30. Am J Physiol v.271 no.3 Pt 2 ACh dilates pial arterioles in endothelial and neuronal NOS knockout mice by NO-dependent mechanisms Meng W;Ma J;Ayata C;Hara H;Huang PL;Fishman MC;Moskowitz MA
  31. Sheng Li Xue Bao v.53 no.2 Involvement of pressure-related mechanism in activation of inducible nitric oxide synthase Tan DY;Cheng SB;Chen XL;Zhang SM;Yang HZ;Peng X;Tang CS;Zhang C;Meng XZ
  32. Proc Natl Acad Sci v.74 no.8 Nitric oxide activates guanylate cyclase and increases guanosine 3':5'-cyclic monophosphate levels in various tissue preparations Arnold WP;Mittal CK;Katsuki S;Murad F
  33. Am J Physiol v.261 no.6 Pt 2 Effects of NG-nitro-L-arginine methyl ester on renal function and blood pressure Lahera V;Salom MG;Miranda-Guardiola F;Moncada S;Romero JC https://doi.org/10.1152/ajpcell.1991.261.6.C1033
  34. Circulation v.91 no.4 Direct measurements of endothelium-derived nitric oxide release by stimulation of endothelin receptors in rat kidney and its alteration in salt-induced hypertension Hirata Y;Hayakawa H;Suzuki E;Kimura K;Kikuchi K;Nagano T;Hirobe M;Omata M https://doi.org/10.1161/01.CIR.91.4.1229
  35. Am J Physiol v.274 no.5 Pt 2 Dietary sodium affects systemic and renal hemodynamic response to NO inhibition in healthy humans Bech JN;Nielsen CB;Ivarsen P;Jensen KT;Pedersen EB https://doi.org/10.1152/ajpcell.1998.274.4.C914
  36. 正統 金塊要 中國中醫 硏究員
  37. 小兒藥證直訣 錢乙
  38. 바른 方劑學 康舜洙
  39. 奇效良方(三冊) 方賢
  40. 醫方集解 汪仁庵
  41. Life Sci v.54 no.26 Gou-Teng (From Uncaria rhynchophylla Miquel)-induced endothelium-dependent and independent relaxations in the isolated rat aorta Kuramochi T;Chu J;Suga T https://doi.org/10.1016/0024-3205(94)00715-2
  42. Am J Chin Med v.27 no.3-4 Effect of Uncariae ramulus et Uncus on endothelium in spontaneously hypertensive rats Goto H;Shimada Y;Tanigawa K;Sekiya N;Shintani T;Terasawa K https://doi.org/10.1142/S0192415X99000380
  43. Am J Chin Med v.28 no.2 Vasodilator effect of extract prepared from Uncariae ramulus on isolated rat aorta Goto H;Sakakibara I;Shimada Y;Kasahara Y;Terasawa K https://doi.org/10.1142/S0192415X00000246
  44. Nitric Oxide v.3 no.5 The direct release of nitric oxide by gypenosides derived from the herb Gynostemma pentaphyllum Tanner MA;Bu X;Steimle JA;Myers PR https://doi.org/10.1006/niox.1999.0245
  45. Life Sci v.62 no.6 Galic prevents hypertension induced by chronic inhibition of nitric oxide synthesis Pedraza-Chaverri J;Tapia E;Medina-Campos ON;de los Angeles Granados M;Franco M
  46. 대한흉부외과학회지 v.32 no.2 고혈압에서 혈관 산화질소 합성 동위효소 발현변화 김인광;강대길;이종은;오봉석
  47. Kor J Physiol Pharmacol v.2 Altered renal nitric oxide system in experimental hypertensive rats Yang ES;Lee JU;Kang DG
  48. Biol Chem v.3 no.5 The direct release of nitric oxide by gypenosides derived from the herb Gynostemma pentaphyllum Lahera V;Salom MG;Steimle JA;Myers PR
  49. Hypertens v.25 no.4 pt2 Relation between pressure natriuresis and urinary excretion of nitrate/ nitrite in anesthetized dogs Majid DS;Godfrey M;Grisham MB;Navar LG https://doi.org/10.1161/01.HYP.25.4.860
  50. Hypertens v.23 no.5 Nitric oxide regulates renal hemo- dynamics and urinary sodium excretion in dogs Manning RD Jr;Hu L https://doi.org/10.1161/01.HYP.23.5.619
  51. Kidney Internat v.46 no.1 Endogenous nitric oxide synthesis determines sensitivity to the pressor effect of salt Tolins J.P.;Shultz P.J. https://doi.org/10.1038/ki.1994.264
  52. Am J Physiol v.240 no.6 Mineralocorticoid effects on Na,K-ATPase in individual nephron segments Garg LC;Knepper MA;Burg MB
  53. 생리학 김종규
  54. 인체 생리학 김광진;김창주;김형진;박사훈
  55. 전정판 신장학 서울대학교 의과대학
  56. Pflugers Arch v.329 no.3 The localization of the Na+,K+-ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes Kinne R;Schmitz JE;Kinne-Saffran E https://doi.org/10.1007/BF00586614
  57. Pflugers Arch v.349 no.1 Induction of Na,K-ATPase in the proximal and distal convolution of the rat nephron after uninephrectomy Schmidt U;Dubach UC
  58. J Biol Chem v.269 no.31 Na,K-ATPase Lingrel JB;Kuntzweiler T
  59. J Biol Chem v.263 no.21 Tissue-specific and developmental egulation of rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs Orlowski J, Lingrel JB