Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study on the Anti-angiogenic Activity of KMKKTE

가미계격탕의 혈관형성 저해작용에 관한 연구

  • Lee Hyo Jeong (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee Eun Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Oh Se Soon (Department of Oriental Pathology, Kyunghee University) ;
  • Ahn Kyoo Seok (Department of Oriental Pathology, Kyunghee University) ;
  • Park Young Doo (Graduate School of Biotechnology, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 이효정 (경희대학교 동서의학대학원 동서종양학과) ;
  • 이은옥 (경희대학교 동서의학대학원 동서종양학과) ;
  • 오세순 (경희대학교 한의과대학 합방병리학) ;
  • 안규석 (경희대학교 한의과대학 합방병리학) ;
  • 박영두 (경희대학교 생명공학원) ;
  • 김성훈 (경희대학교 동서의학대학원 동서종양학과)
  • Published : 2004.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Cancer is an intractable disease for humans to overcome. Recently natural products or Oriental prescriptions have been on the spotlight to develop anticancer agents with little side-effects and good efficacy. KamikeKyuktang has been used for the treatment of cancer in Oriental medicine. However, its anti-cancer mechanism still remains unclear. KMKKTE is an ethanol extract of KamikeKyuktang composed of 12 medicinal herbs. Anti-proliferative effects of KMKKT was investigated on Lewis lung carcinoma cell (LLC) and A549 (human lung cancer cells). Half-maximal inhibition of the LLC and A549 cell proliferation by KMKKTE was found approximately 125㎍/㎖ and 250㎍/㎖, respectively. It also effectively inhibited the proliferation of HUVEC cells treated by bFGF and VEGF up to 30% of control at 125㎍/㎖ and the cell migration to 80% at 25 ㎍/㎖ in concentration dependent manner. Tube formation of HUVEC cells on matrigel also was significantly suppressed from 25㎍/㎖ of KMKKTE. Taken together, these results demonstrate that KMKKTE has antiangiogenic activity and be applied to angiogenesis dependent cancers.

Keywords

References

  1. 병리학 대한병리학회
  2. J Biol Chem v.264 SV40-transformed human lung fibroblasts secrete a 92-kDa type Ⅳ collagenase which is identical to that secreted by normal human macrohpages Wilhelm SM;Collier IE;Marmer BL;Eisen AZ;Grant GA;Goldberg GI.
  3. Cell v.88 no.2 Endostatin: an endogenous inhibitor of angiogenesis and tumor growth O'Reilly MS;Boehm T;Shing Y;Fukai N;Vasios G;Lane WS(et al.)
  4. Cell v.79 no.2 Angiostatin: a novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma O'Reilly MS;Holmgren L;Shing Y;Chen C;Rosenthal RA;Moses M(et al.)
  5. Journal of Korean Oriental Oncology v.5 Anti-metastastic effects of Xuefuzhuyutang Lee JH;Shim BS;Ahn KS;Choi SH.
  6. Korean Journal of Oriental Medical Pathology v.14 Study on the Angiogenic Inhibition Effects of Molyaksan Kang DI;Kim SH;Shim BS;Choi SH;Ahn KS.
  7. Korean Journal of Oriental Medical Pathology v.14 Effects of Huoluoxiaolindan on Anti-metastasis Sohn JG;Kim SH;Seok DS;Shim BS;Choi SH;Ahn KS.
  8. Korean Journal of Oriental Physiology & Pathology v.15 Anti-angiogenic effects of Sipjeondaebo-tang Choi H;Park JH;Yu YB;Shim BS;Choi SH;Ahn KS.
  9. Korean Journal of Oriental Physiology & Pathology v.16 Angiogenic inhibition effects of several herbs supplementing Qi and blood Lee JW;Kim HY;Kang H;Yu YB;Shim BS;Choi SH;Ahn KS.
  10. Oncology Research v.8 Inhibition by galloyglucose(GG6-10) of tumor invasion through extracellular matrix and gelatinase-mediated degradation of type IV collagens by metastatic tumor cells Ata, N;Oku, T;Hattori, M;Fujii, H;Nakajima, M;Saiki, I.
  11. J immonol Method v.65 Rapid colormetric assay for celluar growth and survival : application to proliferation and cytotoxicity assay Mosmann T.
  12. Cancer Res. v.48 Evalution of a soluble tetrazolium/ formazan assay for cell growth and drug sensivity in culture using human and other tumor cell lines Scudiero, D.A.;Shoemaker, RH;Pall, KD
  13. v.79 Angiostatin: a novel angiogenesis inhibitor that mediate the suppression of metastasis by a Lewis lung carcinoma cell O' Reilly, M. S.;Holmgren, L.;Chen C.;Rosental, R. A.;Moses, M.;Lane,W.S; Cao, Y.;Sage, E., H.;Folkman, J.
  14. Nature v.376 Angiogenesis mediated by soluble forms E-selctin and vascular cell adhesion molecule-1 Koch, A. E.;Halloran, M. M.;Haskell, C. J.;Shah, M R.;Polverini, P. J
  15. J. cell physiol. v.13 Interaction of endothelial cells with a laminin A chain peptide (SIKVAV) in vitro and induction of angiogenic behavior in vivo Grant, D.S.;Kinsella, J. L.;Fridman, R.;Auerbach, B.;Piasecki, A.;Yamada, Y.; zain, M.;nad Kleinman, H. K.
  16. Thromb Haemost v.92 no.1 Platelet activation, coagulation and angiogenesis in breast and prostate carcinoma Caine GJ;Lip GY;Stonelake PS;Ryan P;Blann AD.
  17. Br J Cancer. v.90 no.12 Antiangiogenic activity of aplidine, a new agent of marine origin Taraboletti G;Poli M;Dossi R;Manenti L;Borsotti P;Faircloth GT;Broggini M; D'Incalci M;Ribatti D;Giavazzi R.
  18. Int J Cancer. v.110 no.3 CNTO 95, a fully human monoclonal antibody that inhibits alphav integrins, has antitumor and antiangiogenic activity in vivo Trikha M;Zhou Z;Nemeth JA;Chen Q;Sharp C;Emmell E;Giles-Komar J; Nakada MT.
  19. Oncol Res v.14 no.4;5 Inhibition of angiogenesis by salmosin expressed in vitro Kim SI;Kim KS;Kim HS;Choi MM;Kim DS;Chung KH;Park YS.
  20. Planta Med. v.68 no.3 Deoxypodophyllotoxin; the cytotoxic and antiangiogenic component from Pulsatilla koreana Kim Y;Kim SB;You YJ;Ahn BZ.