Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Study of Gene-gene Interaction within GNB3, ACE, ADRB3, ADRB2 among Korean Female Subject

한국인 비만 여성의 GNB3, ACE, ADRB3, ADRB2 유전자 다형성간의 상호관계에 관한 연구

  • Choi Hyun (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Bae Hyun su (Department of Physiology, College of Oriental Medicine, KyungHee University, Purimed R&D Institute) ;
  • Hong Moo chang (Department of Physiology, College of Oriental Medicine, KyungHee University) ;
  • Shin Hyun Dae (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, KyungHee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, KyungHee University)
  • 최현 (경희대학교 한의과대학 생리학교실) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨리메드㈜ 기업부설연구소) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신현대 (경희대학교 한의과대학 재활의학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실)
  • Published : 2004.10.01
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Abstract

There have been several reports on the relationship between G protein β3 subunit gene (GNB3), angiotensin converting enzyme gene (ACE), β3-adrenergic receptor gene (ADRB3), and β2-adrenergic receptor gene (ADRB2) genotype and obesity or obesity related disease. The objective of this study was to examine the relationship between the combinations of these four genes' polymorphism and probability of obesity related disease in Korean female subjects. The experimental group was consisted of 85 obese Korean female subjects (body mass index, BMI≥27㎏/㎡). To determine the polymorphism, genomic DNA was isolated, and PCR was performed. Serological examinations (fasting plasma glucose, FPG; aspartate aminotranferase, AST; alanine aminotransferase, ALT; total cholesterol, TC; triglyceride, TG; high density lipoprotein-cholesterol, HDL; low density lipoprotein-choles terol, LDL) were carried by an autoanalyzer and serological methods. BMI, waist circumference (WC), hip circumference and waist hip ratio (WHR) were measured. Consequencely in the analysis with grouping of general genotyping and variant allele carrier/non-carrier, the result was not significantly different within all gene combinations and polymorphic pairings except higher waist circumference in Arg16Arg group of ADRB2 codon16 (P=0.024). And there was no significantly contrast result about age, height, weight, AST and ALT that are index feature of liver and gall bladder disease in polymorphic pairings of gene combinations. However, the statistical analysis of waist-hip ratio and waist circumference that could be recognized as the physical type of obesity showed T-Arg16 pairing carrier in GNB3-ADRB2 codon16 combination had increased WHR and WC significantly (P=0.046 and P=0.015 respectively). Futhermore, the levels of total cholesterol (TC) and low density lipoprotein choresteral (LDL) were significantly lower in C-I pairing of GNB3-ACE combination (P=0.032 and P=0.005). These results suggest that the T-Arg16 pairing carrier in GNB3-ADRB2 codon16 gene might have increased waist circumference and C-I pairing carrier in GNB3-ACE combination have lower possibility of contraction of cardiovascular disease related cholesterol and LDL despite of obese state.

Keywords

References

  1. 대한가정의학회지 v.17 no.9 비만의 유형과 심혈관계질환 위험인자와의 관련성 홍명호;이영미;최윤선;김순덕
  2. BMJ. v.322 no.7287 Obesity genes. Sorensen TI;Echwald SM.
  3. Am. J. Clin. Nutr. v.72 no.SUP.5 Gene-diet interactions in obesity Perusse L;Bouchard C.
  4. Ann. Intern. Med. v.130 no.11 Genes and obesity: Is there reason to change our behaviors? Weinsier RL.
  5. Behav. Genet. v.27 no.4 Genetic and environmental factors in relative body weight and human adiposity Maes HH;Neale MC;Eaves LJ.
  6. Behav. Genet. v.27 no.4 The genetics of obesity: What have genetic studies told us about the environment Hewitt JK.
  7. Ann.Med. v.28 no.1 Genes and obesity Andersson LB.
  8. Science v.280 no.5368 The search for human obesity genes Comuzzie AG;Allison DB.
  9. N. Engl. J. Med. v.322 The body-mass index of twins who have been reared apart Stunkard AJ;Harris JR;Pedersen NL;McClearn GE.
  10. Stroke v.30 no.9 Evaluation of genetic risk factors for silent brain infarction Notsu Y;Nabika T;Park HY;Masuda J;Kobayashi S.
  11. Arterioscler. Thromb. Vasc. Biol. v.19 no.2 Genetic analysis of the thermolabile variant of 5, 10-methylenetetra- hydrofolate reductase as a risk factor for ischemic stroke Harmon DL;Doyle RM;Meleady R;Doyle M;Shields DC;Barry R;Coakley D;Graham IM;Whitehead AS.
  12. Diabetes Care v.24 no.9 Genotype Arg/Arg, but not Trp/Arg, of the Trp64Arg polymorphism of the beta(3)-adrenergic receptor is associated with type 2 diabetes and obesity in a large Japanese sample Oizumi T;Daimon M;Saitoh T;Kameda W;Yamaguchi H;Ohnuma H;Igarashi M;Eguchi H;Manaka H;Tominaga M;Kato T.
  13. Diabetes Care v.24 no.12 ACE gene polymorphism as a prognostic indicator in patients with type 2 diabetes and established renal disease Fava S;Azzopardi J;Ellard S;Hattersley AT.
  14. J. Neurol. Neurosurg. Psychiatry v.67 no.6 Angiotensin converting enzyme and endothelial nitric oxide synthase DNA polymorphisms and late onset Alzheimer's disease Alvarez R;Alvarez V;Lahoz CH;Martinez C;Pena J;Sanchez JM;Guisasola LM;Salas-Puig J;Moris G;Vidal JA;Ribacoba R;Menes BB;Uria D;Coto E.
  15. Diabetologia v.42 no.1 Association of polymorphisms in the beta2- adrenergic receptor gene with obesity, hypertriglyceridaemia, and diabetes mellitus Ishiyama-Shigemoto S;Yamada K;Yuan X;Ichikawa F;Nonaka K.
  16. J. Clin. Endocrinol. Metab. v.84 no.5 The association between Trp64Arg polymorphism of the beta3-adrenergic receptor and autonomic nervous system activity Shihara N;Yasuda K;Moritani T;Ue H;Adachi T;Tanaka H;Tsuda K;Seino Y.
  17. Metabolism v.48 no.11 Association of beta3-adrenergic receptor gene polymorphism with insulin resistance in Japanese-American men Kawamura T;Egusa G;Okubo M;Imazu M;Yamakido M.
  18. Nephrol. Dial. Transplant. v.15 no.9 G protein beta 3 subunit 825T allele, hypertension, obesity, and diabetic nephropathy Siffert W.
  19. J. Am. Soc. Nephrol. v.10 no.9 Worldwide ethnic distribution of the G protein beta3 subunit 825T allele and its association with obesity in Caucasian, Chinese, and Black African individuals Siffert W;Forster P;Jockel KH;Mvere DA;Brinkmann B;Naber C;Crookes R;Du P Heyns A;Epplen JT;Fridey J;Freedman BI;Muller N;Stolke D;Sharma AM;Al Moutaery K;Grosse-Wilde H;Buerbaum B;Ehrlich T;Ahmad HR;Horsthemke B;Du Toit ED;Tiilikainen A;Ge J;Wang Y;Rosskopf D(et al.)
  20. Int. J. Obes. Relat. Metab. Disord. v.24 no.10 Association of dopamine D2 receptor polymorphisms Ser311Cys and TaqIA with obesity or type 2 diabetes mellitus in Pima Indians Jenkinson CP;Hanson R;Cray K;Wiedrich C;Knowler WC;Bogardus C;Baier L.
  21. Hypertension v.36 no.2 Modulation of blood pressure and obesity with the dopamine D2 receptor gene TaqI polymorphism Thomas GN;Tomlinson B;Critchley JA.
  22. Int. J. Obes. Relat. Metab. Disord. v.23 no.3 Linkages and associations between the leptin receptor (LEPR) gene and human body composition in the Quebec Family Study Chagnon YC;Chung WK;Perusse L;Chagnon M;Leibel RL;Bouchard C.
  23. Diabetes Care v.23 no.6 Polymorphism of the tumor necrosis factor-alpha receptor 2 gene is associated with obesity, leptin levels, and insulin resistance in young subjects and diet-treated type 2 diabetic patients Fernandez-Real JM;Vendrell J;Ricart W;Broch M;Gutierrez C;Casamitjana R;Oriola J;Richart C.
  24. Diabetes v.51 no.1 A Novel homozygous missense mutation of melanocortin-4 receptor (MC4R) in a Japanese woman with severe obesity Kobayashi H;Ogawa Y;Shintani M;Ebihara K;Shimodahira M;Iwakura T;Hino M;Ishihara T;Ikekubo K;Kurahachi H;Nakao K
  25. Hypertension v.36 no.6 Interaction of the ACE D allele and the GNB3 825T allele in myocardial infarction Naber CK;Husing J;Wolfhard U;Erbel R;Siffert W.
  26. Hum. Reprod. v.18 no.9 Gene-gene interaction between fetal MTHFR 677C>Tand transcobalamin776C>Gpolymorphisms in human spontaneous abortion Zetterberg H;Zafiropoulos A;Spandidos DA;Rymo L;Blennow K
  27. Obes. Res. v.9 no.SUP.4 Obesity and disease management: effects of weight loss on comorbid conditions Anderson JW;Konz EC.
  28. Prog. Cardiovasc. Nurs. v.16 no.3 Treating obesity: a new target for prevention of coronary heart disease Aronne LJ.
  29. J. Nutr. v.131 no.3 Calorie restriction in obesity: prevention of kidney disease in rodents Stern JS;Gades MD;Wheeldon CM;Borchers AT.
  30. Metabolism v.51 no.7 Significance of beta2-adrenergic receptor gene polymorphism in obesity and type 2 diabetes mellitus in Korean subjects Kim SH;Kim DJ;Seo IA;Min YK;Lee MS;Kim KW;Lee MK.
  31. 당뇨병 v.22 no.4 한국인 비만증에서 β3 아드레날린 수용체 유전자 다형성의 의의 김병준;김성훈;김동준;함종렬;김진석;안규정;정재훈;민용기;이명식;이문규;김광원
  32. 대한비만학회지 v.10 no.2 한국인에서 렙틴수용체 유전자 다형성과 체지방분포 서영성;김대현;이인규
  33. 대한비만학회지 v.10 no.2 한국인 비만자에서 PPAR${\gamma}$2 유전자 Pro12Ala 다형성과 체지방분포 서영성;김대현;김승기;이인규;김흥식
  34. Nucleic Acids Res. v.19 no.19 A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies Lahiri DK;Nurnberger JI Jr.
  35. Obes. Res. v.10 no.SUP.2 Management of obesity as a chronic disease: nonpharmacologic, pharma cologic, and surgical options Fujioka K.
  36. Arch. Intern. Med. v.160 no.7 Overweight, obesity, and health risk. National Task Force on the Prevention and Treatment of Obesity National Institute of Diabetes and Digestive and Kidney Diseases in U.S.A.
  37. JAMA. v.282 no.16 The disease burden associated with overweight and obesity Must A;Spadano J;Coakley EH;Field AE;Colditz G;Dietz WH.
  38. Int. J. Obes. Relat. Metab. Disord. v.25 no.10 Is obesity a disease? Heshka S;Allison DB.
  39. Int. J. Obes. Relat. Metab. Disord. v.22 no.5 Excess energy intake and low energy expenditure as predictors of obesity Roberts SB;Leibel RL.
  40. Metabolism v.50 no.12 Are there persons who are obese, but metabolically healthy? Sims EA.
  41. JAMA. v.256 no.1 A twin study of human obesity Stunkard AJ;Foch TT;Hrubec Z.
  42. Int. J. Obes. Relat. Metab. Disord. v.22 no.1 Adoption study of environmental modifications of the genetic influences on obesity Sorensen TI;Holst C;Stunkard AJ.
  43. J. Clin. Invest. v.100 no.12 Human beta-2 adrenoceptor gene polymorphisms are highly frequent in obesity and associate with altered adipocyte beta-2 adrenoceptor function Large V;Hellstrom L;Reynisdottir S;Lonnqvist F;Eriksson P;Lannfelt L;Arner P.
  44. Nature v.395 no.6704 Leptin and the regulation of body weight in mammals Friedman JM;Halaas JL.
  45. 東醫壽世保元註解 신홍일(註解)
  46. 알기쉬운 사상의학 송일병
  47. Curr. Hypertens. Rep. v.2 no.1 The role of beta(2)-adrenergic receptor variation in human hypertension Bray MS;Boerwinkle E.
  48. Hypertension v.37 no.5 Beta(2)-adrenergic receptor gene variation and hypertension in subjects with type 2 diabetes Bengtsson K;Orho-Melander M;Melander O;Lindblad U;Ranstam J;Rastam L; Groop L.
  49. Hypertension v.37 no.3 G-protein beta(3) subunit gene (GNB3) 825T allele is associated with enhanced renal perfusion in early hypertension Zeltner R;Delles C;Schneider M;Siffert W;Schmieder RE.
  50. Biochem. Biophys. Res. Commun. v.286 no.4 Analysis of 825C/T polymorphism of G protein beta3 subunit in obese/diabetic Japanese Ohshiro Y;Ueda K;Wakasaki H;Takasu N;Nanjo K.
  51. Nature v.359 no.6396 Deletion polymorphism in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction Cambien F;Poirier O;Lecerf L;Evans A;Cambou JP;Arveiler D;Luc G;Bard JM;Bara L;Ricard S;Tiret L;Amouyel P;Alhenc-Gelas F;Soubrier F.
  52. Circulation v.90 no.2 Plasma level and gene polymorphism of angiotensin- converting enzyme in relation to myocardial infarction Cambien F;Costerousse O;Tiret L;Poirier O;Lecerf L;Gonzales MF;Evans A;Arveiler D;Cambou JP;Luc G.
  53. Eur. J. Endocrinol. v.132 no.4 Beta 3-adrenoceptor: an update Giacobino JP.
  54. Annu. Rev. Med. v.48 Brown adipose tissue, beta 3-adrenergic receptors, and obesity Lowell BB;Flier JS.
  55. 대한비만학회지 v.10 no.3 Role of ${\beta}$3 Adrenoreceptor 최웅환
  56. Am. J. Respir. Crit. Care Med. v.158 no.5 The ${\beta}$ adrenoceptor Johnson M.
  57. Pulm. Pharmacol. v.8 no.1 Implications of genetic variability of human beta 2-adrenergic receptor structure Green SA;Turki J;Hall IP;Liggett SB.
  58. J. Intern. Med. v.245 no.6 Adrenoceptor genes in human obesity Arner P;Hoffstedt J.
  59. J. Intern. Med. v.245 no.3 The different effects of a Gln27Glu beta 2-adrenoceptorgene polymorphism on obesity in males and in females Hellstrom L;Large V;Reynisdottir S;Wahrenberg H;Arner P.